Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,813,955	T→G	I381L (ATC→CTC)	gshA ←	gamma‑glutamate‑cysteine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,813,955	0	T	G	100.0%	129.3 / NA	36	I381L (ATC→CTC)	gshA	gamma‑glutamate‑cysteine ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (36/0);  total (36/0)

GTAACGGGAACTGTGCGGTTTCGCAGCCGATACCCAGCGTCAGACCCGGTTTGCGACCTTCGAGGATCA  >  W3110S.gb/2813889‑2813957                                                                   |   gTAACGGGAACTGTGCGGTTTCGCAGCCGATCCCCAGCGTCAGACCCGGTTTGCGACCTTCGAAGAGCa  >  1:635795/1‑69 (MQ=255) gTAACGGGAACTGTGCGGTTTCGCAGCCGATACCCAGCGTCAGACCCGGTTTGCGACCTTCGAAGAGCa  >  1:58989/1‑69 (MQ=255) gTAACGGGAACTGTGCGGTTTCGCAGCCGATACCCAGCGTCAGACCCGGTTTGCGACCTTCGAAGAGCa  >  1:346159/1‑69 (MQ=255) gTAACGGGAACTGTGCGGTTTCGCAGCCGATACCCAGCGTCAGACCCGGTTTGCGACCTTCGAAGAGCa  >  1:364108/1‑69 (MQ=255) gTAACGGGAACTGTGCGGTTTCGCAGCCGATACCCAGCGTCAGACCCGGTTTGCGACCTTCGAAGAGCa  >  1:427585/1‑69 (MQ=255) gTAACGGGAACTGTGCGGTTTCGCAGCCGATACCCAGCGTCAGACCCGGTTTGCGACCTTCGAAGAGCa  >  1:470462/1‑69 (MQ=255) gTAACGGGAACTGTGCGGTTTCGCAGCCGATACCCAGCGTCAGACCCGGTTTGCGACCTTCGAAGAGCa  >  1:508231/1‑69 (MQ=255) gTAACGGGAACTGTGCGGTTTCGCAGCCGATACCCAGCGTCAGACCCGGTTTGCGACCTTCGAAGAGCa  >  1:512591/1‑69 (MQ=255) gTAACGGGAACTGTGCGGTTTCGCAGCCGATACCCAGCGTCAGACCCGGTTTGCGACCTTCGAAGAGCa  >  1:563042/1‑69 (MQ=255) gTAACGGGAACTGTGCGGTTTCGCAGCCGATACCCAGCGTCAGACCCGGTTTGCGACCTTCGAAGAGCa  >  1:577569/1‑69 (MQ=255) gTAACGGGAACTGTGCGGTTTCGCAGCCGATACCCAGCGTCAGACCCGGTTTGCGACCTTCGAAGAGCa  >  1:258806/1‑69 (MQ=255) gTAACGGGAACTGTGCGGTTTCGCAGCCGATACCCAGCGTCAGACCCGGTTTGCGACCTTCGAAGAGCa  >  1:744038/1‑69 (MQ=255) gTAACGGGAACTGTGCGGTTTCGCAGCCGATACCCAGCGTCAGACCCGGTTTGCGACCTTCGAAGAGCa  >  1:755047/1‑69 (MQ=255) gTAACGGGAACTGTGCGGTTTCGCAGCCGATACCCAGCGTCAGACCCGGTTTGCGACCTTCGAAGAGCa  >  1:852622/1‑69 (MQ=255) gTAACGGGAACTGTGCGGTTTCGCAGCCGATACCCAGCGTCAGACCCGGTTTGCGACCTTCGAAGAGCa  >  1:887903/1‑69 (MQ=255) gTAACGGGAACTGTGCGGTTTCGCAGCCGATACCCAGCGTCAGACCCGGTTTGCGACCTTCGAAGAGCa  >  1:935692/1‑69 (MQ=255) gTAACGGGAACTGTGCGGTTTCGCAGCCGATACCCAGCGTCAGACCCGGTTTGCGACCTTCGAAGAGCa  >  1:965603/1‑69 (MQ=255) gTAACGGGAACTGTGCGGTTTCGCAGCCGATACCCAGCGTCAGACCCGGTTTGCGACCTTCGAAGAGCa  >  1:990613/1‑69 (MQ=255) gTAACGGGAACTGTGCGGTTTCGCAGCCGATACCCAGCGTCAGACCCGGTTTGCGACCTTCGAAGAGCa  >  1:1280745/1‑69 (MQ=255) gTAACGGGAACTGTGCGGTTTCGCAGCCGATACCCAGCGTCAGACCCGGTTTGCGACCTTCGAAGAGCa  >  1:1026503/1‑69 (MQ=255) gTAACGGGAACTGTGCGGTTTCGCAGCCGATACCCAGCGTCAGACCCGGTTTGCGACCTTCGAAGAGCa  >  1:1049040/1‑69 (MQ=255) gTAACGGGAACTGTGCGGTTTCGCAGCCGATACCCAGCGTCAGACCCGGTTTGCGACCTTCGAAGAGCa  >  1:1081660/1‑69 (MQ=255) gTAACGGGAACTGTGCGGTTTCGCAGCCGATACCCAGCGTCAGACCCGGTTTGCGACCTTCGAAGAGCa  >  1:1087125/1‑69 (MQ=255) gTAACGGGAACTGTGCGGTTTCGCAGCCGATACCCAGCGTCAGACCCGGTTTGCGACCTTCGAAGAGCa  >  1:1104544/1‑69 (MQ=255) gTAACGGGAACTGTGCGGTTTCGCAGCCGATACCCAGCGTCAGACCCGGTTTGCGACCTTCGAAGAGCa  >  1:1139448/1‑69 (MQ=255) gTAACGGGAACTGTGCGGTTTCGCAGCCGATACCCAGCGTCAGACCCGGTTTGCGACCTTCGAAGAGCa  >  1:1186320/1‑69 (MQ=255) gTAACGGGAACTGTGCGGTTTCGCAGCCGATACCCAGCGTCAGACCCGGTTTGCGACCTTCGAAGAGCa  >  1:1260601/1‑69 (MQ=255) gTAACGGGAACTGTGCGGTTTCGCAGCCGATACCCAGCGTCAGACCCGGTTTGCGACCTTCGAAGAGCa  >  1:100987/1‑69 (MQ=255) gTAACGGGAACTGTGCGGTTTCGCAGCCGATACCCAGCGTCAGACCCGGTTTGCGACCTTCGAAGAGCa  >  1:1342548/1‑69 (MQ=255) gTAACGGGAACTGTGCGGTTTCGCAGCCGATACCCAGCGTCAGACCCGGTTTGCGACCTTCGAAGAGCa  >  1:1374750/1‑69 (MQ=255) gTAACGGGAACTGTGCGGTTTCGCAGCCGATACCCAGCGTCAGACCCGGTTTGCGACCTTCGAAGAGCa  >  1:1387441/1‑69 (MQ=255) gTAACGGGAACTGTGCGGTTTCGCAGCCGATACCCAGCGTCAGACCCGGTTTGCGACCTTCGAAGAGCa  >  1:1433521/1‑69 (MQ=255) gTAACGGGAACTGTGCGGTTTCGCAGCCGATACCCAGCGTCAGACCCGGTTTGCGACCTTCGAAGAGCa  >  1:1446352/1‑69 (MQ=255) gTAACGGGAACTGTGCGGTTTCGCAGCCGATACCCAGCGTCAGACCCGGTTTGCGACCTTCGAAGAGCa  >  1:1459368/1‑69 (MQ=255) gTAACGGGAACTGTGCGGTTTCGCAGCCGATACCCAGCGTCAGACCCGGTTTGCGACCTTCGAAGAGCa  >  1:1603731/1‑69 (MQ=255) gTAACGGGAACTGTGCGGTTTCGCAGCCGATACCCAGCGTCAGACCCGGTTTGCGACCTTCGAAGAGCa  >  1:234231/1‑69 (MQ=255)                                                                   |   GTAACGGGAACTGTGCGGTTTCGCAGCCGATACCCAGCGTCAGACCCGGTTTGCGACCTTCGAGGATCA  >  W3110S.gb/2813889‑2813957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑