Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,840,761 A→G E372E (GAA→GAG)  ascB → cryptic 6‑phospho‑beta‑glucosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,840,7610AG100.0% 280.2 / NA 76E372E (GAA→GAG) ascBcryptic 6‑phospho‑beta‑glucosidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (76/0);  total (76/0)

TATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAG  >  W3110S.gb/2840735‑2840803
                          |                                          
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCt                                    >  1:817580/1‑35 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAAttt                 >  1:1139702/1‑54 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:342632/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:473800/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:449877/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:44437/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:436380/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:422951/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:415228/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:401148/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:394835/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:392422/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:495821/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:338498/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:329031/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:328496/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:327928/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:327364/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:318979/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:1008988/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:798925/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:994138/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:972173/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:938481/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:905107/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:869657/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:863963/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:853160/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:801939/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:48672/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:770624/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:714935/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:702326/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:639269/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:519011/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:517132/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:510749/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:26824/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:1224676/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:1400616/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:1348420/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:1345189/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:1306623/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:1300357/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:1289451/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:1276299/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:1250566/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:1234236/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:140296/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:1218506/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:1191044/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:1177910/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:1148489/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:1091508/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:1084221/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:1018774/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:1015827/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:1546471/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:241160/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:223582/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:208862/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:20180/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:193599/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:1584194/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:1573523/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:1568461/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:265398/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:1544895/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:152595/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:1499672/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:1477983/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:147143/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:1466950/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag  >  1:1406680/1‑69 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCga   >  1:37164/1‑68 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACCGCCTGGGCGCAAAAGATGAATTtgct              >  1:43417/1‑57 (MQ=255)
                          |                                          
TATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAG  >  W3110S.gb/2840735‑2840803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑