Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,194,164 T→C T416A (ACG→GCG)  rfaE ← fused heptose 7‑phosphate kinase and heptose 1‑phosphate adenyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,194,1640TC100.0% 575.5 / NA 153T416A (ACG→GCG) rfaEfused heptose 7‑phosphate kinase and heptose 1‑phosphate adenyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (153/0);  total (153/0)

Only 100 of 154 total aligned reads displayed.

CAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGTCG  >  W3110S.gb/3194123‑3194192
                                         |                            
cagcagATCTGGCAAGCTCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGCCACTACCCAGTc   >  1:548427/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCgcgt                            >  1:1364809/1‑44 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCa                    >  1:402008/1‑52 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGacac            >  1:844669/1‑60 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGt    >  1:559493/1‑68 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGt    >  1:721435/1‑68 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:598336/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:654130/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:651957/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:64523/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:640408/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:1000738/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:426241/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:621149/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:614304/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:60096/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:475745/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:577086/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:569169/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:690783/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:440095/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:545520/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:447621/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:509716/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:503300/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:480044/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:799455/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:99012/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:96923/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:967584/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:957911/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:914770/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:908893/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:879965/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:854550/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:845408/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:82941/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:808502/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:67654/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:797834/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:794842/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:767423/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:763795/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:732744/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:729770/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:70816/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:691771/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:407918/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:678508/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:1197364/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:395646/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:1441211/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:1411287/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:1403788/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:1342439/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:1304013/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:1297796/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:129770/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:1288526/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:1252770/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:1232365/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:1450614/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:117761/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:1170292/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:1156697/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:1153419/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:113147/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:112851/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:1121183/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:1111521/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:1065169/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:1061766/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:1047179/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:1553358/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:392603/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:340209/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:31819/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:302887/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:279106/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:256640/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:235857/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:212501/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:194081/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:1577971/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:1565119/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:1458588/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:1552836/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:1534290/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:1533388/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:1487817/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:1487303/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:147525/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:1462404/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:1458781/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACACAGTc   >  1:958273/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACAACCCAGTc   >  1:698125/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCAGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:224994/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGCGCAGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:660333/1‑69 (MQ=255)
cagcagATCTGGCAAGATCCCGGCGATCAAGAGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc   >  1:610737/1‑69 (MQ=255)
 agcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCg  >  1:1242094/1‑69 (MQ=255)
                                         |                            
CAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGTCG  >  W3110S.gb/3194123‑3194192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑