Predicted mutation | ||||||
---|---|---|---|---|---|---|
evidence | seq id | position | mutation | annotation | gene | description |
RA | W3110S.gb | 3,807,619 | G→A | A580A (GCG→GCA) | yicI → | predicted alpha‑glucosidase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 3,807,619 | 0 | G | A | 99.4% | 586.1 / ‑4.7 | 158 | A580A (GCG→GCA) | yicI | predicted alpha‑glucosidase |
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (0/157); total (0/158) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 |
Only 100 of 158 total aligned reads displayed.
TCTGTATCGTGAAGCTGCGCGTGCGAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGTT > W3110S.gb/3807595‑3807663 | tCTGTATCGTGAAGCTGTACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:1058265/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCGAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:1344835/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGTTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:1571660/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:675548/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:705441/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:647648/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:620050/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:595644/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:589162/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:5661/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:52985/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:511243/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:48948/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:480497/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:471901/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:417944/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:416873/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:392425/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:384543/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:370623/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:353520/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:351731/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:340454/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:327422/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:322223/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:319399/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:84876/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:992022/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:984278/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:983300/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:959915/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:957488/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:941186/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:932203/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:931973/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:90581/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:884510/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:869059/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:696885/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:846131/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:842297/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:830921/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:810495/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:7887/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:77464/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:76843/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:755045/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:734352/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:731590/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:29565/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:1228096/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:1383829/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:1001157/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:1343377/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:1328180/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:1312731/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:1307317/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:1303893/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:1299171/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:1291380/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:1285746/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:1253328/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:136325/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:1218447/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:1217144/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:1216934/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:1178518/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:1093524/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:1080260/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:1062763/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:1039249/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:1037782/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:1001809/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:1397689/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:288701/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:255875/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:241725/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:221949/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:210235/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:188124/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:17418/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:1601963/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:1583034/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:1559256/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:1504000/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:149908/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:1448519/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:1438752/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:1428071/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:1424774/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:1421971/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:1414798/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:1385214/69‑1 (MQ=255) tCTGTATCGTGAAGCTGCACGCGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:745886/69‑1 (MQ=255) tCTGTATCGTGAAGCTGAACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:160854/69‑1 (MQ=255) cTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:1117580/68‑1 (MQ=255) cTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:257233/68‑1 (MQ=255) cTGTATCGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:303312/68‑1 (MQ=255) cGTGAAGCTGCACGTGCAAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGtt < 1:1146488/62‑1 (MQ=255) gCTGCACGTGCAAACGCGCGGGGTACGCCTATGATGCGGGCCATGATGATGGAGtt < 1:874309/56‑1 (MQ=255) | TCTGTATCGTGAAGCTGCGCGTGCGAACGCGCGGGGTACGCCGATGATGCGGGCCATGATGATGGAGTT > W3110S.gb/3807595‑3807663 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |