Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,066,786	C→T	intergenic (+144/‑129)	asd → / → glgB	aspartate‑semialdehyde dehydrogenase, NAD(P)‑binding/1,4‑alpha‑glucan branching enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,066,786	0	C	T	100.0%	339.8 / NA	93	intergenic (+144/‑129)	asd/glgB	aspartate‑semialdehyde dehydrogenase, NAD(P)‑binding/1,4‑alpha‑glucan branching enzyme
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/61);  total (32/61)

CTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCG  >  W3110S.gb/4066752‑4066833                                   |                                                cTTTAATGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATg                >  1:656977/1‑68 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATg                >  1:499340/1‑68 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATg                >  1:1331421/1‑68 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATg                >  1:1393243/1‑68 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATg                >  1:1471711/1‑68 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATg                >  1:1556296/1‑68 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATg                >  1:1590761/1‑68 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATg                >  1:178158/1‑68 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATg                >  1:192292/1‑68 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATg                >  1:193822/1‑68 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATg                >  1:353691/1‑68 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATg                >  1:39841/1‑68 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATg                >  1:1025060/1‑68 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATg                >  1:580509/1‑68 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATg                >  1:635783/1‑68 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATg                >  1:752210/1‑68 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATg                >  1:806985/1‑68 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATg                >  1:832541/1‑68 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATg                >  1:832946/1‑68 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATg                >  1:870633/1‑68 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATg                >  1:94522/1‑68 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATg                >  1:972963/1‑68 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATg                >  1:1015547/1‑68 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATg                >  1:1151842/1‑68 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATg                >  1:1190836/1‑68 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATg                >  1:1134986/1‑68 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATg                >  1:1035745/1‑68 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATg                >  1:1034465/1‑68 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATg                >  1:1222501/1‑68 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATg                >  1:1111546/1‑68 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATg                >  1:1270469/1‑68 (MQ=255) cTTTAAGGGTGGCTGAATACACGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATg                >  1:1117276/1‑68 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:547286/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:538807/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:61484/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:620345/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:629066/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:43516/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:634780/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:490730/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:1102215/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:65187/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:1064682/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:657701/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:684549/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:686307/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:711455/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:1055462/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:760608/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:782042/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:104919/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:829914/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:1044412/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:861532/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:873388/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:91833/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:941716/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:942266/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:1027375/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:966346/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:1561787/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:194856/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:1140539/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:184360/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:177835/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:166588/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:161924/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:1210392/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:1565490/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:212387/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:1536966/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:1507615/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:1232403/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:1265947/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:1378576/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:1268636/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:1324380/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:4869/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:22466/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:259731/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:262244/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:292182/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:302499/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:336791/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:34589/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:360379/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:374279/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:389588/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:1130013/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:412877/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:420250/69‑1 (MQ=255)              tGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:474855/69‑1 (MQ=255)               gAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTgcg  <  1:523433/68‑1 (MQ=255)                                   |                                                CTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCG  >  W3110S.gb/4066752‑4066833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑