Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,404,063	G→A	A44T (GCC→ACC)	miaA →	delta(2)‑isopentenylpyrophosphate tRNA‑adenosine transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,404,063	0	G	A	100.0%	87.6 / NA	25	A44T (GCC→ACC)	miaA	delta(2)‑isopentenylpyrophosphate tRNA‑adenosine transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/0);  total (25/0)

GAGTTGATAAGCGTTGATTCTGCCCTTATTTACAAAGGGATGGATATCGGGACGGCGAAGCCGAACGC  >  W3110S.gb/4404042‑4404109                      |                                               gagTTGATAAGCGTTGATTCTACCCTTATTTACAAAGGGATGGATATCGGGACGGCGAAGCCGAACGc  >  1:245410/1‑68 (MQ=255) gagTTGATAAGCGTTGATTCTACCCTTATTTACAAAGGGATGGATATCGGGACGGCGAAGCCGAACGc  >  1:784626/1‑68 (MQ=255) gagTTGATAAGCGTTGATTCTACCCTTATTTACAAAGGGATGGATATCGGGACGGCGAAGCCGAACGc  >  1:783762/1‑68 (MQ=255) gagTTGATAAGCGTTGATTCTACCCTTATTTACAAAGGGATGGATATCGGGACGGCGAAGCCGAACGc  >  1:77450/1‑68 (MQ=255) gagTTGATAAGCGTTGATTCTACCCTTATTTACAAAGGGATGGATATCGGGACGGCGAAGCCGAACGc  >  1:722400/1‑68 (MQ=255) gagTTGATAAGCGTTGATTCTACCCTTATTTACAAAGGGATGGATATCGGGACGGCGAAGCCGAACGc  >  1:569488/1‑68 (MQ=255) gagTTGATAAGCGTTGATTCTACCCTTATTTACAAAGGGATGGATATCGGGACGGCGAAGCCGAACGc  >  1:554283/1‑68 (MQ=255) gagTTGATAAGCGTTGATTCTACCCTTATTTACAAAGGGATGGATATCGGGACGGCGAAGCCGAACGc  >  1:550542/1‑68 (MQ=255) gagTTGATAAGCGTTGATTCTACCCTTATTTACAAAGGGATGGATATCGGGACGGCGAAGCCGAACGc  >  1:398903/1‑68 (MQ=255) gagTTGATAAGCGTTGATTCTACCCTTATTTACAAAGGGATGGATATCGGGACGGCGAAGCCGAACGc  >  1:397091/1‑68 (MQ=255) gagTTGATAAGCGTTGATTCTACCCTTATTTACAAAGGGATGGATATCGGGACGGCGAAGCCGAACGc  >  1:376464/1‑68 (MQ=255) gagTTGATAAGCGTTGATTCTACCCTTATTTACAAAGGGATGGATATCGGGACGGCGAAGCCGAACGc  >  1:269348/1‑68 (MQ=255) gagTTGATAAGCGTTGATTCTACCCTTATTTACAAAGGGATGGATATCGGGACGGCGAAGCCGAACGc  >  1:259980/1‑68 (MQ=255) gagTTGATAAGCGTTGATTCTACCCTTATTTACAAAGGGATGGATATCGGGACGGCGAAGCCGAACGc  >  1:1017500/1‑68 (MQ=255) gagTTGATAAGCGTTGATTCTACCCTTATTTACAAAGGGATGGATATCGGGACGGCGAAGCCGAACGc  >  1:232154/1‑68 (MQ=255) gagTTGATAAGCGTTGATTCTACCCTTATTTACAAAGGGATGGATATCGGGACGGCGAAGCCGAACGc  >  1:22894/1‑68 (MQ=255) gagTTGATAAGCGTTGATTCTACCCTTATTTACAAAGGGATGGATATCGGGACGGCGAAGCCGAACGc  >  1:160656/1‑68 (MQ=255) gagTTGATAAGCGTTGATTCTACCCTTATTTACAAAGGGATGGATATCGGGACGGCGAAGCCGAACGc  >  1:1553357/1‑68 (MQ=255) gagTTGATAAGCGTTGATTCTACCCTTATTTACAAAGGGATGGATATCGGGACGGCGAAGCCGAACGc  >  1:1528751/1‑68 (MQ=255) gagTTGATAAGCGTTGATTCTACCCTTATTTACAAAGGGATGGATATCGGGACGGCGAAGCCGAACGc  >  1:1297032/1‑68 (MQ=255) gagTTGATAAGCGTTGATTCTACCCTTATTTACAAAGGGATGGATATCGGGACGGCGAAGCCGAACGc  >  1:1250530/1‑68 (MQ=255) gagTTGATAAGCGTTGATTCTACCCTTATTTACAAAGGGATGGATATCGGGACGGCGAAGCCGAACGc  >  1:1120934/1‑68 (MQ=255) gagTTGATAAGCGTTGATTCTACCCTTATTTACAAAGGGATGGATATCGGGACGGCGAAGCCGAACGc  >  1:1115215/1‑68 (MQ=255) gagTTGATAAGCGTTGATTCTACCCTTATTTACAAAGGGATGGATATCGGGACGGCGAAGCCGAACGc  >  1:1105096/1‑68 (MQ=255) gagTTGATAAGCGTTGATTCTACCCTTATTTACAAAGGGATGGATATCGGGACGGCGAAGCCGAACGc  >  1:1027267/1‑68 (MQ=255)                      |                                               GAGTTGATAAGCGTTGATTCTGCCCTTATTTACAAAGGGATGGATATCGGGACGGCGAAGCCGAACGC  >  W3110S.gb/4404042‑4404109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑