Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 606,831 T→A intergenic (‑225/‑228) ybdK ← / → hokE gamma‑glutamyl:cysteine ligase/toxic polypeptide, small

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb606,8310TA100.0% 487.8 / NA 135intergenic (‑225/‑228)ybdK/hokEgamma‑glutamyl:cysteine ligase/toxic polypeptide, small
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (135/0);  total (135/0)

Only 100 of 135 total aligned reads displayed.

GTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATTAAATGAGGAGTGAGAAGTCCGAAACAGGACTC  >  W3110S.gb/606795‑606863
                                    |                                
gtaaTGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:138200/4‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGTGTGAGAAGTCCGAAACAGGACTc  >  1:559262/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGaa                  >  1:1104756/1‑53 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGa     >  1:1558115/1‑66 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACt   >  1:1010636/1‑68 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACt   >  1:326905/1‑68 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACt   >  1:851877/1‑68 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACt   >  1:1533598/1‑68 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:329693/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1603681/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:53629/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:521041/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:488209/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:487587/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:455090/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:426262/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:420289/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:404971/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:349880/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:183399/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:979084/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:169307/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:31555/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:288958/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:252204/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:24567/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:241001/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:179612/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:183413/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:754760/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1004712/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:939670/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:921504/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:91821/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:899007/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:888051/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:866928/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:866211/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:856921/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:765049/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:758511/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:569859/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:740352/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:708563/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:699750/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:687417/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:669010/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:667551/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:660404/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:645355/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:637554/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:590434/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:577248/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1154887/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1236072/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1229837/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1226428/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1222732/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1221008/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1212267/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1208513/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1204439/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1180150/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1179502/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1168713/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1245382/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1137238/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1123511/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1112619/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1110027/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1086070/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1075443/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1069334/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1049758/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1033115/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1032561/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1017927/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1352697/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1552966/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1480129/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1477989/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1470169/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1444367/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1436122/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1432565/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1403722/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1384640/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1367075/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1361377/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1591749/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1336892/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1314941/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1290666/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1284613/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1270858/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1265509/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1253686/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1249445/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGTGAGAAGTCCGAAACAGGACTc  >  1:1246796/1‑69 (MQ=255)
gTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATAAAATGAGGAGAGAGAAGTCCGAAACAGGACt   >  1:1289480/1‑68 (MQ=255)
                                    |                                
GTCATGCAGAAAAAAACGGGTTCAGCTTCAGTTGATTAAATGAGGAGTGAGAAGTCCGAAACAGGACTC  >  W3110S.gb/606795‑606863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑