| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,663,570 | Δ1 bp | 22.9% | coding (552/882 nt) | yfhR → | predicted peptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,663,570 | 0 | T | . | 22.9% | 99.2 / 36.9 | 48 | coding (552/882 nt) | yfhR | predicted peptidase |
| Reads supporting (aligned to +/- strand): ref base T (20/17); new base . (7/4); total (27/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.45e-01 | |||||||||||
CGGGCAGAGTATTGGCGGGGCGAATATTCTGGATGTTATTGGTCGGGGTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGC > W3110S.gb/2663504‑2663633 | cGGGCAGAGTATTGGCGGGGCGAATATTCTGGATGTTATTGGTCGGGGTGATCGTGAAGGCATACGTGCgg > 1:3362206/1‑71 (MQ=255) ggCAGAGTATTGGCGGGGCGAATATTCTGGATGTTATTGGTCGGGGTGATCGTGAAGGCATACGTGCGGTg < 1:1720418/71‑1 (MQ=255) cAGAGTATTGGCGGGGCGAATATTCTGGATGTTATTGGTCGGGGTGATCGTGAAGGCATACG‑GCGGTGATc < 1:3431856/71‑1 (MQ=255) gagTATTGGCGGGGCGAATATTCTGGATGTTATTGGTCGGGGTGATCGTGAAGGCATACGTGCGGTGATcc > 1:658972/1‑71 (MQ=255) agTATTGGCGGGGCGAATATTCTGGATGTTATTGGTCGGGGTGATCGTGAAGGCATACG‑GCGGTGATCCTc < 1:2471410/71‑1 (MQ=255) gTATTGGCGGGGCGAATATTCTGGATGTTATTGGTCGGGGTGAACGTGAAGGCATACGTGCGGTGATCCTc < 1:1481158/71‑1 (MQ=255) tATTGGCGGGGCGAATATTCTGGATGTTATTGGTCGGGGTGATCGTGAAGGCATACGTGCGGTGATCCTc < 1:334406/70‑1 (MQ=255) ttGGCGGGGCGAATATTCTGGATGTTATTGGTCGGGGTGATCGTGAAGGCATACGTGCgg > 1:1210449/1‑60 (MQ=255) gCGAATATTCTGGATGTTATTGGTCGGGGTGATCGTGAAGGCATACGTGCGGTGATc > 1:1595359/1‑57 (MQ=255) gCGAATATTCTGGATGTTATTGGTCGGGGTGATCGTGAAGGCATACG‑GCGGTGATCCTCGa > 1:132096/1‑61 (MQ=255) gCGAATATTCTGGATGTTATTGGTCGGGGTGATCGTGAAGGCATACG‑GCGGTGATCCTCGACTCCACAttt < 1:2104256/71‑1 (MQ=255) cGAATATTCTGGATGTTATTGGTCGGGGTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACAttt < 1:3018642/71‑1 (MQ=255) cGAATATTCTGGATGTTATTGGTCGGGGTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACAttt < 1:2315452/71‑1 (MQ=255) gAATATTCTGGATGTTATTGGTCGGGGTGATCGTGAAGGCATACG‑GCGGTGAt > 1:1017778/1‑53 (MQ=255) gAATATTCTGGATGTTATTGGTCGGGGTGATCGTGAAGGCATACG‑GCGGTGAt > 1:1011597/1‑53 (MQ=255) aaTATTCTGGATGTTATTGGTCGGGGTGATCGTGAAGGCATACGTGc < 1:2176987/47‑1 (MQ=255) tatTCTGGATGTTATTGGTCGGGGTGATCGTGAAGGCATACGTGCGGTGa < 1:4049340/50‑1 (MQ=255) tatTCTGGATGTTATTGGTCGGGGTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCct > 1:3442082/1‑71 (MQ=255) ttCTGGATGTTATTGGTCGGGGTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCctct < 1:1843502/71‑1 (MQ=255) ttCTGGATGTTATTGGTCGGGGTGATCGTGAAGGCATACG‑GCGGTGATCCTCGACTCCACATTTGCctct > 1:1715283/1‑70 (MQ=255) tCTGGATGTTATTGGTCGGGGTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCcaca > 1:2532102/1‑61 (MQ=255) cTGGATGTTATTGGTCGGGGTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTa > 1:717059/1‑71 (MQ=255) cTGGATGTTATTGGTCGGGGTGATAGTGAAGGCATACG‑GCGGTGATCCTCGACTCCACATTTGCCTCtt > 1:767536/1‑69 (MQ=255) gATGGTATTGGTCGGGGTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTAt < 1:926100/69‑1 (MQ=255) ttATTGGTCGGGGTGATCGTGAAGGCATACGTGCGGt < 1:2348160/37‑1 (MQ=255) ttATTGGTCGGGGTGATCGTGAAGGCATACGTGCGGt < 1:1262465/37‑1 (MQ=255) ttATTGGTCGGGGTGATCGTGAAGGCATACGTGCGGTg < 1:3496193/38‑1 (MQ=255) tATTGGTCGGGGTGATCGTGAAGGCATACGTGCGGTGATCCTCGAc > 1:3912301/1‑46 (MQ=255) aTTGGTCGGGGTGATCGTGAAGGCATACGTGCGGTGAt > 1:4188426/1‑38 (MQ=255) aTTGGTCGGGGTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTa > 1:1249214/1‑62 (MQ=255) ggTCGGGGTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAAc < 1:1870028/65‑1 (MQ=255) ggTCGGGGTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGc > 1:1410193/1‑71 (MQ=255) gTCGGGGTGATCGTGAAGGCATACG‑GCGGTGATCCTCGa > 1:2332967/1‑39 (MQ=38) aTCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCtt > 1:986041/1‑48 (MQ=255) aTCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCtt > 1:3909642/1‑48 (MQ=255) aTCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAAt > 1:3389120/1‑70 (MQ=255) tCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTa < 1:1240864/48‑1 (MQ=255) cGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCaa > 1:4172154/1‑52 (MQ=255) cGTGAAGGCATACG‑GCGGTGATACTCGACTCCACATTTGCCTCTTa > 1:1616795/1‑46 (MQ=38) tGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAAcc < 1:2734360/52‑1 (MQ=255) gAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATg > 1:753870/1‑46 (MQ=255) gCATACG‑GCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGc < 1:4086088/70‑1 (MQ=255) cATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCg > 1:4015709/1‑68 (MQ=255) tACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCa < 1:1071288/41‑1 (MQ=255) tACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGAt < 1:4106874/61‑1 (MQ=255) aCGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGAt > 1:3918189/1‑60 (MQ=255) aCGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGAt > 1:2581284/1‑60 (MQ=255) gTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGccaa > 1:4361896/1‑49 (MQ=255) | CGGGCAGAGTATTGGCGGGGCGAATATTCTGGATGTTATTGGTCGGGGTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGC > W3110S.gb/2663504‑2663633 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |