| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,338,560 | Δ1 bp | 17.9% | coding (71/669 nt) | basR ← | DNA‑binding response regulator in two‑component regulatory system with BasS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,338,560 | 0 | C | . | 17.9% | 159.8 / 24.9 | 56 | coding (71/669 nt) | basR | DNA‑binding response regulator in two‑component regulatory system with BasS |
| Reads supporting (aligned to +/- strand): ref base C (30/16); new base . (6/4); total (36/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01 | |||||||||||
AGGCTGTAATGACCTGCCTCAAGGCTTTGTTCCGCCATCCGCGCGGTTGTCACGCTATCGCACGCGTAGCCTTCGGTTTGCGCCGCCAGAATCAGTCCCTGCAATAACAGCGTATCGTCTTCAACAATCAGAATTTTC > W3110S.gb/4338491‑4338628 | aGGCTGTAATGACCTGCCTCAAGGCTTTGTTCCGCCATCCGCGCGGTTGTCACGCTATCGCACGCGTAGcc < 1:967092/71‑1 (MQ=255) ggCTGTAATGACCTGCCTCAAGGCTTTGTTCCGCCATCCGCGCGGTTGTCACGCTATCGCACGCGTAGc > 1:3027106/1‑69 (MQ=255) ggCTGTAATGACCTGCCTCAAGGCTTTGTTCCGCCATCCGCGCGGTTGTCACGCTATCGCACGCGTAGCCt > 1:1963610/1‑71 (MQ=255) ggCTGTAATGACCTGCCTCAAGGCTTTGTTCCGCCATCCGCGCGGTTGTCACGATATCGCACGCGTAGctt < 1:1073970/71‑3 (MQ=255) tGTAATGACCTGCCTCAAGGCTTTGTTCCGCCATCCGCGCGGTTGTCACGCTATCGCACGCGTAGCCTTCg > 1:4519052/1‑71 (MQ=255) aaTGACCTGCCTCAAGGCTTTGTTCCGCCATCCGCGCGGTTGTCACGCTATCGCACGCGTAGCCTTCGGtt > 1:4123867/1‑71 (MQ=255) aaTGACCTGCCTCAAGGCTTTGTTCCGCCATCCGCGCGGTTGTCACGCTATCGCACGCGTAGCCTTCGGtt > 1:2927161/1‑71 (MQ=255) aaTGACCTGCCTCAAGGCTTTGTTCCGCCATCCGCGCGGTTGTCACGCTATCGCACGCGTAGCCTTCGGtt > 1:4129020/1‑71 (MQ=255) aTGACCTGCCTCAAGGCTTTGTTCCGCCATCCGCGCGGTTGTCACGCTATCGCACGCGTAGCCTTCGGttt > 1:3629724/1‑71 (MQ=255) aTGACCTGCCTCAAGGCTTTGTTCCGCCATCCGCGCGGTTGTCACGCTATCGCACGCGTAGCCTTCGGttt > 1:1968466/1‑71 (MQ=255) aTGACCTGCCTCAAGGCTTTGTTCCGCCATCCGCGCGGTTGTCACGCTATCGCACGCGTAGCCTTCGGttt > 1:3677164/1‑71 (MQ=255) aTGACCTGCCTCAAGGCTTTGTTCCGCCATCCGCGCGGTTGTCACGCTATCGCACGCGTAG‑CTTCgg > 1:2868124/1‑67 (MQ=255) aCCTGCCTCAAGGCTTTGTTCCGCCATCCGCGCGGTTGTCACGCTATCGCACGCGTAGCCTTCg > 1:1321641/1‑64 (MQ=255) aCCTGCCTCAAGGCTTTGTTCCGCCATCCGCGCGGTTGTCACGCTATCGCACGCGTAGCCTTCGGTTTgcg > 1:2015008/1‑71 (MQ=255) cTGCCTCAAGGCTTTGTTCCGCCATCCGCGCGGTTGTCACGCTATCGCACGCGTAG‑CTTCGGTTTGcgccg > 1:2182283/1‑71 (MQ=255) gCCTCAAGGCTTTGTTCCGCCATCCGCGCGGTTGTCACGCTATCGCACGCGTAG‑CTTCGGTTTGcgccgc > 1:2152348/1‑70 (MQ=255) ccTCAAGGCTTTGTTCCGCCATCCGCGCGGTTGTCACGCTATCGCACGCGTAGCCTTCGGTTTGcgccgc > 1:3835096/1‑70 (MQ=255) ccTCAAGGCTTTGTTCCGCCATCCGCGCGGTTGTCACGCTATCGCACGCGTAGCCTTCGGTTTGcgccgc > 1:1965843/1‑70 (MQ=255) aaGGCTTTGTTCCGCCATCCGCGCGGTTGTCACGCTATCGCAGGCGTAGCCTTCGGTTTGCGCCGCCAg < 1:514961/69‑1 (MQ=255) aaGGCTTTGTTCCGCCATCCGCGCGGTTGTCACGCTATCGCACGCGTAG‑CTTCGGTTTGCGCCGCCAGAAt < 1:529430/71‑1 (MQ=255) aGGCTTTGTTCCGCCATCCGCGCGGTTGTCACGCTATCGCACGCGTAGCCTTCGGTTTGCGCCGCCAGAAt < 1:949737/71‑1 (MQ=255) cTTTGTTCCGCCATCCGCGCGGTTGTCACGCTATCGCACGCGTAGCCTTCGGTTTGCGCCGCCAGAATCAg > 1:1093584/1‑71 (MQ=255) tttGTTCCGCCATCCGCGCGGTTGTCACGCTATCGCACGCGTAG‑CTTCGGTTTGCGCCGCCAGAATCAGTc > 1:1436466/1‑71 (MQ=255) tGTTCCGCCATCCGCGCGGTTGTCACGCTATCGCACGCGTAGCCTTCGGTTTGCGCCGCCAGAATCAGTc < 1:388390/70‑1 (MQ=255) tGTTCCGCCATCCGCGCGGTTGTCACGCTATCGCACGCGTAG‑CTTCGGTTTGCGCCGCCAGAATCAGTc < 1:120024/69‑1 (MQ=255) gTTCCGCCATCCGCGCGGTTGTCACGCTATCGCACGCGTAGCCTTCGGTTTGCGCCGCCAGAATCAGt < 1:4064072/68‑1 (MQ=255) gTTCCGCCATCCGCGCGGTTGTCACGCTATCGCACGCGTAGCCTTCGGTTTGCGCCGCCAGAATCAGTccc > 1:1641143/1‑71 (MQ=255) ccATCCGCGCGGTTGTCACGCTATCGCACGCGTAGCCTTCGGTTTGCGCCGCCAGAATCAGTCCCTGCAAt < 1:210547/71‑1 (MQ=255) ccATCCGCGCGGTTGTCACGCTATCGCACGCGTAGCCTTCGGTTTGCGCCGCCAGAATCAGTCCCTGCAAt < 1:3047134/71‑1 (MQ=255) ccATCCGCGCGGTTGTCACGCTATCGCAAGCGTAGCCTTCGGTTTGCGCCGCCAGAATCAGTCCCTGCAAt < 1:3700506/71‑1 (MQ=255) cATCCGCGCGGTTGTCACGCTATCGCACGCGTAGCCTTCGGTTTGCGCCGCCAGAATCAGTCCCTGCAAt < 1:1907496/70‑1 (MQ=255) ccGCGCGGTTGTCACGCTATCGCACGCGTAGCCTTCGGttt < 1:3749509/41‑1 (MQ=255) ccGCGCGGTTGTCACGCTATCGCACGCGTAGCCTTCGGTTTGCGCCGCCAGAATCAGTCCCTGCAAt > 1:4031930/1‑67 (MQ=255) ccGCGCGGTTGTCACGCTATCGCACGCGTAGCCTTCGGTTTGCGCCGCCAGAATCAGTCCCTGCAAt > 1:3300295/1‑67 (MQ=255) cgcgcgGTTGTCACGCTATCGCACGCGTAGCCTTCGGTTTGCGCCGCCAGAATCAGTCCCTGCAATaa > 1:3554428/1‑68 (MQ=255) cgcgcgGTTGTCACGCTATCGCACGCGTAGCCTTCGGTTTGCGCCGCCAGAATCAGTCCCTGCAATAACAg > 1:3263558/1‑71 (MQ=255) cgcgcgGTTGTCACGCTATCGCACGCGTAGCCTTCGGTTTGCGCCGCCAGAATCAGTCCCTGCAATAACAg > 1:766645/1‑71 (MQ=255) cgcgGTTGTCACGCTATCGCACGCGTAGCCTTCGGTTTGCGCCGCCAGAATCa > 1:4141228/1‑53 (MQ=255) cgGTTGTCACGCTATCGCACGCGTAGCCTTCGGTTTGCGCCGCCAGAATCAGTCCCTGCAATAACAGCGTa < 1:3438868/71‑1 (MQ=255) gTTGTCACGCTATCGCACGCGTAG‑CTTCGGTTTGCGCCGCCAGAATCAGTCCCTGCAATAACAGCGTATCg < 1:3145993/71‑1 (MQ=255) gTCACGCTATCGCACGCGTAGCCTTCGGTTTGCGCCGCCAGAAt < 1:3318857/44‑1 (MQ=255) gTCACGCTATCGCACGCGTAGCCTTCGGTTTGCGCCGCCAGAATCAGTccc > 1:4519136/1‑51 (MQ=255) aCGCTATCGCACGCGTAGCCTTCGGTTTGCGCCGCCAGAATCAGTCCCTGCAATAAc > 1:2939515/1‑57 (MQ=255) aCGCTATCGCACGCGTAGCCTTCGGTTTGCGCCGCCAGAATCAGTCCCTGCAATAACAGCGTATCGTCtt > 1:2137421/1‑70 (MQ=255) tCGCACGCGTAGCCTTCGGTTTGCGCCGCCAGAATCAGTCCCTGCAATAACAGCGTATCGTCTTCAACAAt > 1:3898477/1‑71 (MQ=255) tCGCACGCGTAGCCTTCGGTTTGCGCCGCCAGAATCAGTCCCTGCAATAACAGCGTATCGTCTTCAACAAt < 1:162114/71‑1 (MQ=255) tCGCACGCGTAGCCTTCGGTTTGCGCCGCCAGAATCAGTCCCTGCAATAACAGCGTATCGTCTTCAACAAt > 1:2238243/1‑71 (MQ=255) gCACGCGTAGCCTTCGGTTTGCGCCGCCAGAATCa > 1:4121424/1‑35 (MQ=255) cACGCGTAGCCTTCGGTTTGCGCCGCCAGAATCAGTCCCTGCAATaa > 1:377286/1‑47 (MQ=255) cACGCGTAG‑CTTCGGTTTGCGCCGCCAGAATCAGTCCCTGCAATAACAGCGTATCGTCTTCAACAATCAg > 1:4151931/1‑70 (MQ=255) cgcgTAGCCTTCGGTTTGCGCCGCCAGAATCAGTCCCTGCAATa > 1:1202500/1‑44 (MQ=255) gcgTAG‑CTTCGGTTTGCGCCGCCAGAATCAGTCCCTGCAATAACAGCGTATCGTCTTCAACAATCAGAAtt < 1:4254281/71‑1 (MQ=255) cgTAGCCTTCGGTTTGCGCCGCCAGAATCAGTCCCTGCAATAACAGCGTATCGTCTTCAACAATc > 1:2393239/1‑65 (MQ=255) cgTAG‑CTTCGGTTTGCGCCGCCAGAATCAGTCCCTGCAATAACAGCGTATc > 1:3717450/1‑51 (MQ=255) tAGCCTTCGGTTTGCGCCGCCAGAATCAGTCCCTGCAATAACAGCGTATc < 1:227790/50‑1 (MQ=255) tAGCCTTCGGTTTGCGCCGCCAGAATCAGTCCCTGCAATAACAGCGTATCGTCTTCAACAATCAGAAtttt < 1:3516549/71‑1 (MQ=255) atcccTTCGGTTTGCGCCGCCAGAATCAGTCCCTGCAATAACAGCGTATCGTCTTCAACAATCAGAAtttt < 1:2211240/68‑1 (MQ=255) aGCCTTCGGTTTGCGCCGCCAGAATCAGTCCCTGCaa > 1:2791581/1‑37 (MQ=255) gCCTTCGGTTTGCGCCGCCAGAATCAGTCCCTGCAATAACAGCGTATCGTCTTCAACAATCAGAATTttc < 1:3866140/70‑1 (MQ=255) gCCTTCGGTTTGCGCCGCCAGAATCAGTCCCTGCAATAACAGCGTATCGTCTTCAACAATCAGAATTttc < 1:1047992/70‑1 (MQ=255) | AGGCTGTAATGACCTGCCTCAAGGCTTTGTTCCGCCATCCGCGCGGTTGTCACGCTATCGCACGCGTAGCCTTCGGTTTGCGCCGCCAGAATCAGTCCCTGCAATAACAGCGTATCGTCTTCAACAATCAGAATTTTC > W3110S.gb/4338491‑4338628 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |