| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,701,562:1 | +C | 14.7% | coding (494/1593 nt) | malX → | fused maltose and glucose‑specific PTS enzyme IIBC components |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,701,562 | 1 | . | C | 14.7% | 83.5 / 20.5 | 34 | coding (494/1593 nt) | malX | fused maltose and glucose‑specific PTS enzyme IIBC components |
| Reads supporting (aligned to +/- strand): ref base . (13/16); new base C (3/2); total (16/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01 | |||||||||||
GTCGATCGATACCGGGATCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTCGGCGGTACGCGCTTCGTACCAATTATCTCCTCG > W3110S.gb/1701494‑1701632 | gTCGATCGATACCGGGATCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑c > 1:887825/1‑70 (MQ=255)gTCGATCGATACCGGGATCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑c > 1:3471972/1‑70 (MQ=255) gatcgatACCGGGATCCTCGGTGCGGTGATCGCCGATATTATCGTCTGGATGCTGCATGAGCGTTT‑CCat < 1:3918500/70‑1 (MQ=255) tcgatACCGGGATCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑CCataa > 1:873615/1‑70 (MQ=255) tcgatACCGGGATCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑CCataa < 1:2041805/70‑1 (MQ=255) cgatACCGGGATCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑CCataata > 1:3699572/1‑71 (MQ=255) atACCGGGATCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑cccat < 1:3840628/66‑4 (MQ=255) atACCGGGATCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATc < 1:2744340/71‑1 (MQ=255) tCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCg < 1:93499/71‑1 (MQ=255) gtgcggtgATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑CCataata < 1:1673920/54‑1 (MQ=255) tgcggtgATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCGGATgcgc < 1:3999145/71‑1 (MQ=255) cggtgATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑cc < 1:3034601/45‑1 (MQ=255) tgATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCa < 1:2051229/71‑1 (MQ=255) gTATTATCGTCTGGATGCTGCATGAGCGTTT‑CCat < 1:3633409/35‑1 (MQ=255) ttatCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCt < 1:1264323/43‑1 (MQ=255) ttatCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCGGAt < 1:4168363/50‑1 (MQ=255) ttatCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTcggcgg < 1:868483/70‑1 (MQ=255) tatCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTCGGCGGTa > 1:3445955/1‑71 (MQ=255) atCGTCTGGATGCTGCATGAGCGTTTCCCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTCGGCGGTa > 1:2513303/1‑71 (MQ=255) atCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGcc > 1:4572064/1‑44 (MQ=255) atCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTCGGCGGTAc > 1:92558/1‑71 (MQ=255) tCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCt > 1:4286490/1‑52 (MQ=255) tCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCAtt > 1:2815459/1‑58 (MQ=255) tCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCAtt > 1:3681376/1‑58 (MQ=255) tCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTcggcgg < 1:3315212/67‑1 (MQ=255) cGTCTGGATGCTGCATGAGCGTTTCCCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTCGGCGGTAcg > 1:4086160/1‑71 (MQ=255) cGTCTGGATGCTGCATGAGCGTTTCCCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTCGGCGGTAc > 1:3380691/1‑70 (MQ=255) cGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGc > 1:3819086/1‑37 (MQ=255) cGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGc > 1:3594162/1‑37 (MQ=255) tCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTCGGCGGTACgcgc < 1:2847681/71‑1 (MQ=255) cTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTCGGCGGTACgcgc < 1:1741423/70‑1 (MQ=255) gATGCTGCATGAGCGTTTCCCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTCGGCGGTACGCGCtt < 1:3960725/70‑1 (MQ=255) ctgcATGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTCGGCGGTACGCGCTTc > 1:2631702/1‑66 (MQ=255) cATGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTCGGCGGTACGCGCTTCGTACCAAt < 1:3683737/71‑1 (MQ=255) cATGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTCGGCGGTACGCGCTTCGTACCAAt > 1:3161650/1‑71 (MQ=255) tGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTCGGCGGTACGCGCTTCGTACCAAtt < 1:2875303/70‑1 (MQ=255) aGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTCGGCGGTa > 1:3236981/1‑51 (MQ=255) gCGTTTCCCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTCGGCTGTACGCGCTTCGTACCAATTAtc < 1:1112401/71‑1 (MQ=255) t‑CCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTCGGCGGTACGCGCTTCGTACCAATTATCTCCTCg > 1:2100430/1‑71 (MQ=255) | GTCGATCGATACCGGGATCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTCGGCGGTACGCGCTTCGTACCAATTATCTCCTCG > W3110S.gb/1701494‑1701632 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |