| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,824,533:1 | +G | 100% | pseudogene (666/687 nt) | rph → | ECK3633:JW3618:b3643; ribonuclease PH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,824,533 | 1 | . | G | 100.0% | 113.5 / NA | 34 | pseudogene (666/687 nt) | rph | ECK3633:JW3618:b3643; ribonuclease PH |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (20/14); total (20/14) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch. | |||||||||||
TGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCATCTTGTTGGCTCTGGCCCGA‑GGGGAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCAAACTGATTTTTA > W3110S.gb/3824466‑3824589 | tGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGa < 1:666498/68‑1 (MQ=255)tGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑ggg > 1:169494/1‑71 (MQ=255) aCGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGgaatcga < 1:609740/71‑1 (MQ=255) ggCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGgaatcgaat > 1:1935389/1‑71 (MQ=255) cAGAAGGCGAGCCGTTCACCAATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGgaatcgaatc > 1:1808500/1‑70 (MQ=255) aaGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGATGGCCCGAGGGGGAATCGAATCCAt > 1:1088362/1‑70 (MQ=255) gAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGc < 1:3731694/71‑1 (MQ=255) gAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGc < 1:235365/71‑1 (MQ=255) cACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTg > 1:1138796/1‑59 (MQ=255) cACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGACGCAg > 1:1660170/1‑70 (MQ=255) aCCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGACGCAGaa < 1:777572/71‑1 (MQ=255) cATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGACGCAGAAggc > 1:2850328/1‑71 (MQ=255) cATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGACGCAGAAggc > 1:2841980/1‑71 (MQ=255) cATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGACGCAGAAggc < 1:2608057/71‑1 (MQ=255) tGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGgaatcgaatc < 1:86225/48‑1 (MQ=255) gAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGACGCAGAAggcg > 1:539004/1‑69 (MQ=255) aaGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGc > 1:3362324/1‑55 (MQ=255) aaGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGACGCAGAAggcggc < 1:1986870/70‑1 (MQ=255) aaGAGCTACTCACCTTGTTGGCGCTGCCCCGAGGGGGAATCGAATCCATTGTAGCGACGCAGAAggcggc > 1:659279/1‑70 (MQ=255) agagCTACTCACCTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGACGCAg < 1:3714994/61‑1 (MQ=255) tgCTACTCACCTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGACGCAg < 1:1028504/58‑1 (MQ=255) agCTACTCACCTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGACGCa > 1:1297176/1‑58 (MQ=255) agCTACTCACCTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGACGCa > 1:1291733/1‑58 (MQ=255) agCTACTAACCTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGACGCAGaa > 1:3995946/1‑61 (MQ=255) cTACTCACCTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGACGCAGa > 1:4638611/1‑58 (MQ=255) accTTGTTGGCTCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCAAACTg < 1:3058868/69‑1 (MQ=255) accTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGa > 1:409568/3‑46 (MQ=255) accTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCAAACt > 1:3672084/3‑70 (MQ=255) accTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCAAACTg < 1:1037568/69‑1 (MQ=255) ccTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTGt < 1:3866153/39‑1 (MQ=37) cTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTGt < 1:611246/39‑1 (MQ=37) tgttgGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCAAACTGAttt > 1:3410898/1‑71 (MQ=255) tgttgGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCAAACTGAtt > 1:1621828/1‑70 (MQ=255) gttgGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGa > 1:4026852/1‑41 (MQ=37) gttgGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCaaa > 1:1304892/1‑63 (MQ=255) ggCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCAAACTGATTTTTa < 1:1136973/70‑1 (MQ=255) | TGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCATCTTGTTGGCTCTGGCCCGA‑GGGGAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCAAACTGATTTTTA > W3110S.gb/3824466‑3824589 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |