New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 64196340 (0.870)15 (0.360)
+AGAA		 4/88 NT 29.6% coding (653/1239 nt) ybdR predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding
?W3110S.gb = 4186745 38 (0.830)coding (255/420 nt) pioO part of gsp divergon involved in type II protein secretion

TCGGTGCGGAACAGA‑TTTTTGTTGTTGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/641936‑641963
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTGTTTTTTGAAG  <  W3110S.gb/4186745‑4186733
                             ||||             
aCGGTGAGAAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGA    <  1:1374633/43‑1
 CGGTGAGAAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTG     <  1:3242642/42‑1
 CGGTGAGAAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTG     <  1:3862677/42‑1
  GGTGAGAAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAA   >  1:108194/1‑43
  GGTGAGAAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAA   >  1:2943343/1‑43
  GGTGAGAAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAA   >  1:298811/1‑43
        aAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAAG  >  1:4429170/2‑38
        aAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAA   >  1:4275952/2‑37
        aAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAA   <  1:553512/36‑1
        aAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTG     <  1:3704405/34‑1
        aAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTG     <  1:4640796/34‑1
        aAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTG     <  1:547936/34‑1
         AACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAAG  >  1:1284419/1‑37
         AACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAA   >  1:368963/1‑36
          ACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAAG  >  1:285023/1‑36
                             ||||             
TCGGTGCGGAACAGA‑TTTTTGTTGTTGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/641936‑641963
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTGTTTTTTGAAG  <  W3110S.gb/4186745‑4186733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.