New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 122552919 (0.410)13 (0.370)
+GCAAAAGATTA		 3/74 NT 36.0% intergenic (+45/+327) ycgI/minE hypothetical protein/cell division topological specificity factor
?W3110S.gb 3671920 = 41 (0.890)coding (870/1266 nt) rhlB ATP‑dependent RNA helicase
Rejected: Coverage evenness skew score above cutoff.

AGTTTTCGTTTACAGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/1225514‑1225529
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CGCGCAGAAAAAAC  >  W3110S.gb/3671920‑3671933
                |||||||||||              
ttTTTTCGTTTACAGGGCAAAAGATTACGCGCAGAAAAAA   <  1:2691055/38‑1
ttTTTTCGTTTACAGGGCAAAAGATTACGCGCAGAAAAAA   <  1:673524/38‑1
ttTTTTCGTTTACACGGCAAAAGATTACGCGCAGAAAAAA   <  1:2116567/38‑1
  TTTTCGTTTACAGGGCAAAAGATTACGCGCAGAAAAAAa  >  1:265832/1‑38
  TTTTCGTTTACAGGGCAAAAGATTACGCGCAGAAAAAAa  >  1:581979/1‑38
  TTTTCGTTTACAGGGCAAAAGATTACGCGCAGAAAAAA   >  1:1371807/1‑38
  TTTTCGTTTACAGGGCAAAAGATTACGCGCAGAAAAAA   >  1:1885612/1‑38
  TTTTCGTTTACAGGGCAAAAGATTACGCGCAGAAAAAA   <  1:2912322/38‑1
  TTTTCGTTTACAGGGCAAAAGATTACGCGCAGAAAAAA   <  1:3017575/38‑1
  TTTTCGTTTACAGGGCAAAAGATTACGCGCAGAAAAA    >  1:3110344/1‑37
  TTTTCGTTTACAGGGCAAAAGATTACGCGCAGAAAAA    >  1:3334002/1‑37
  TTTTCGTTTACAGGGCAAAAGATTACGCGCAGAAAAA    <  1:3522631/37‑1
     TCGTTTACAGGGCAAAAGATTACGCGCAGAAAAAAa  <  1:960128/36‑2
                |||||||||||              
AGTTTTCGTTTACAGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/1225514‑1225529
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CGCGCAGAAAAAAC  >  W3110S.gb/3671920‑3671933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.