| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | W3110S.gb | 1800961 = | 30 (0.650) | 5 (0.140) +CGTATTTTCCG |
3/74 | NT | 14.7% | coding (24/45 nt) | pheM | phenylalanyl‑tRNA synthetase operon leader peptide |
| ? | W3110S.gb | = 4445415 | 45 (0.980) | coding (483/1344 nt) | ytfL | predicted inner membrane protein | |||||
| Rejected: Coverage evenness skew score above cutoff. | |||||||||||
TATTTTCCGCTTC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ < W3110S.gb/1800973‑1800961‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTTCCGTATTTTCA < W3110S.gb/4445415‑4445402 ||||||||||| TATTTTCCGCTTCCGTATTTTCCGCTTCCGTATTTTC > 1:397445/1‑37TATTTTCCGCTTCCGTATTTTCCGCTTCCGTATTTTC < 1:505019/37‑1 ATTTTCCGCTTCCGTATTTTCCGCTTCCGTATTTTC > 1:2797557/1‑36 TTTTCCGCTTCCGTATTTTCCGCTTCCGTATTTTCc > 1:4023843/1‑35 TTTTCCGCTTCCGTATTTTCCGCTTCCGTATTTTCc < 1:850923/36‑2 ||||||||||| TATTTTCCGCTTC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ < W3110S.gb/1800973‑1800961‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTTCCGTATTTTCA < W3110S.gb/4445415‑4445402 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |