Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC W3110S.gb 3,261,134 Δ1,199 bp 100% insH[tdcD] insH, [tdcD]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ W3110S.gb 3261134–3262332 3262332 1–1199 46 [1] [0] 45 insH–[tdcD] insH,[tdcD]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 32611330 (0.000)29 (0.690) 20/88 NT 100% intergenic (‑59/‑69) tdcE/insH pyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase/IS5 element protein
?W3110S.gb 3262333 = 0 (0.000)coding (1183/1296 nt) tdcD propionate kinase/acetate kinase C, anaerobic

AATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/3261060‑3261133
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAAT  >  W3110S.gb/3262333‑3262399
                                                                                                                                             
AATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGT                                                                        >  1:4521370/1‑71
 ATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTT                                                                       <  1:4029461/71‑1
 ATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGT                                                                        >  1:1730663/1‑70
    TCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAAC                                                                    >  1:2450775/1‑71
          TTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAA                                                                     <  1:1945331/64‑1
            CATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCT                                                             >  1:3474482/1‑70
            CATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCT                                                             >  1:270086/1‑70
              TGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAAC                                                                    <  1:963822/61‑1
               GAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTA                                                            <  1:3862434/68‑1
                AAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATG                                                        <  1:4581755/71‑1
                AAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATG                                                        <  1:4426699/71‑1
                  AAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATG                                                        <  1:3424780/69‑1
                     TAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGG                                                   >  1:3092104/1‑71
                       ATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCA                                                 >  1:2499700/1‑71
                       ATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTT                                                                 >  1:1001163/1‑55
                             CTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTT                                                                 <  1:3035944/49‑1
                              TACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCA                                             <  1:3329527/68‑1
                              TACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTT                                                                       <  1:3708712/42‑1
                                CAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGC                                         <  1:389146/70‑1
                                  ATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGC                                                               >  1:2300776/1‑46
                                   TACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACat                                                                  >  1:363686/1‑40
                                    ACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCA                                    >  1:3209886/1‑71
                                    ACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCA                                    >  1:4011228/1‑71
                                     CTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATG                                                        <  1:1045687/50‑1
                                     CTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAAC                                                                    >  1:1376738/1‑38
                                       TCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTT                                 >  1:3272929/1‑71
                                        CAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTT                                 <  1:3305869/70‑1
                                        CAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATAGCATCCAAAGCAATCATTTT                                >  1:3691496/1‑71
                                         AACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTT                                <  1:746113/70‑1
                                         AACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTT                                <  1:563968/70‑1
                                           CTAAATTATGCAAATTCTGCGGGCACGTTAACTTT                                                                 <  1:2533566/35‑1
                                            TAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATG                                                        <  1:4256895/43‑1
                                             AAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTT                           >  1:3786258/1‑71
                                                 TATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATG                                                        <  1:831074/38‑1
                                                   TGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCG                         <  1:971606/67‑1
                                                    GCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCG                         <  1:4170091/66‑1
                                                     CAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAACCAATCATTTTTTCTTCGTTAGTC                   <  1:1019335/71‑1
                                                        ATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGA                <  1:3562326/71‑1
                                                        ATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTA                      <  1:135032/65‑1
                                                          TCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTT                              >  1:1487563/1‑55
                                                           CTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAAT              <  1:1445549/70‑1
                                                                   GCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCAC      >  1:202000/1‑70
                                                                    CGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAA    <  1:138687/71‑1
                                                                     GTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAA   <  1:2916752/71‑1
                                                                      TTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAAT  <  1:1475323/71‑1
                                                                        AACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAA            >  1:4572661/1‑59
                                                                        AACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTA                      >  1:2902949/1‑49
                                                                        AACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTT                                 >  1:2511376/1‑38
                                                                        AACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTT                                 >  1:574724/1‑38
                                                                                                                                             
AATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/3261060‑3261133
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAAT  >  W3110S.gb/3262333‑3262399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.