Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC W3110S.gb 3,746,913 Δ1,199 bp 100% [tnaB][tnaB] [tnaB], insH, [tnaB]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ W3110S.gb 3746913–3748110 3748111 2–1199 78 [0] [0] 78 [tnaB]–[tnaB] [tnaB],insH,[tnaB]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 37469120 (0.000)50 (1.190) 35/88 NT 100% coding (71/447 nt) tnaB tryptophan transporter of low affinity
?W3110S.gb 3748112 = 0 (0.000)coding (871/984 nt) tnaB tryptophan transporter of low affinity

TAAACAGGTCCGCCAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/3746841‑3746912
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttagAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAACGATTTCACCAGCGAATC  >  W3110S.gb/3748112‑3748181
                                                                                                                                              
TAAACAGGTCCGCCAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGT                                                                           >  1:2453570/1‑69
 AAACAGGTCCGCCAGATAATCGAACAACCCCAGCTTGACACCAAAGAACGAACTGGCAACAGCTAAGTTA                                                                         <  1:1565688/70‑1
 AAACAGGTCCGCCAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTA                                                                         <  1:217163/70‑1
 AAACAGGTCCGCCAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGT                                                                           >  1:4412699/1‑68
 AAACAGGTCCGCCAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGT                                                                           >  1:2122224/1‑68
 AAACAGGTCCGCCAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGT                                                                           >  1:4511316/1‑68
    CAGGTCCGCCAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAG                                                                      >  1:851909/1‑70
      GGTCCGCCAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAAC                                                                   <  1:2619287/71‑1
        TCCGCCAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACAC                                                                 <  1:553059/71‑1
          CGCCAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACC                                                                <  1:1600828/70‑1
            CCAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAG                                                              >  1:3612909/1‑70
                  AATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAG                                                           <  1:2283681/67‑1
                   ATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGC                                                             <  1:3971908/64‑1
                    TCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAA                                                       <  1:4382756/69‑1
                    TCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAG                                                              <  1:2526620/62‑1
                     CGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAAC                                                    >  1:2653352/1‑71
                         CAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGA                                                >  1:3986667/1‑71
                         CAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGA                                                >  1:3733973/1‑71
                         CAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGA                                                <  1:3659248/71‑1
                          AACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGG                                                          <  1:2668932/60‑1
                          AACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAG                                                                      <  1:569412/48‑1
                            CCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAA                                             >  1:2073179/1‑71
                            CCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGAT                                               <  1:1643446/69‑1
                            CCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAA                                                                    >  1:1030901/1‑48
                             CCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAG                                                                      <  1:3637313/45‑1
                             CCCAGCGTAACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAG                                                                      <  1:2448123/45‑1
                               CAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGC                                          >  1:2716145/1‑71
                               CAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATA                                              >  1:449426/1‑67
                               CAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAA                                                                    >  1:4489598/1‑45
                               CAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAA                                                                    >  1:4006904/1‑45
                               CAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAG                                                                      <  1:3889882/43‑1
                                AGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCC                                         >  1:2495058/1‑71
                                 GCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCA                                                            <  1:3598652/51‑1
                                 GCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAG                                                                      <  1:1885505/41‑1
                                   GTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACAGCAGCAGGCAAAACTCGAT                                               <  1:3723651/62‑1
                                   GTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTG                                      >  1:1184192/1‑71
                                   GTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAA                                                       <  1:497461/54‑1
                                   GTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAA                                                                    >  1:4384783/1‑41
                                   GTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAA                                                                    >  1:3393791/1‑41
                                    TGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGC                                     >  1:4230158/1‑71
                                     GACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAA                                                     >  1:1142389/1‑54
                                     GACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAG                                                                      <  1:1766717/37‑1
                                     GACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAG                                                                      <  1:1938318/37‑1
                                     GACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAG                                                                      <  1:4110477/37‑1
                                      ACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAA                                             >  1:1677015/1‑61
                                      ACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCA                                                            >  1:4330684/1‑46
                                      ACACCAAAGAACGAACTGGAAACAGCTAAGTTAGAG                                                                      <  1:108650/36‑1
                                       CACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCA                                                               <  1:1374296/42‑1
                                       CACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAA                                                                    >  1:3387360/1‑37
                                        ACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTC                                                  <  1:2876403/54‑1
                                        ACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTC                                                  <  1:677836/54‑1
                                        ACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTC                                                  <  1:4521199/54‑1
                                         CCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGAT                                               <  1:4461154/56‑1
                                         CCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGC                                                             >  1:2334294/1‑42
                                          CAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTG                               >  1:3255928/1‑71
                                           AAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGG                              >  1:3414510/1‑71
                                           AAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGG                              >  1:3869495/1‑71
                                           AAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAG                                                           >  1:549536/1‑42
                                           AAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGT                             <  1:1054897/71‑1
                                            AAGAACGTACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGT                             <  1:1861022/71‑1
                                             AGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAAT                                            <  1:1767908/55‑1
                                             AGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAAT                                            <  1:1783972/55‑1
                                              GAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGC                           >  1:3595134/1‑71
                                              GAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGC                           >  1:3319613/1‑71
                                              GAACGAACTGGCAACAGCTAAGTTACAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGT                                  >  1:652300/1‑64
                                               AACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCC                          <  1:3971404/71‑1
                                               AACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCA                                                        <  1:4588743/41‑1
                                                ACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAAC                                                    >  1:2103290/1‑44
                                                     CTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTC                                                  <  1:802578/41‑1
                                                        GCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGC                                          >  1:3568116/1‑46
                                                            CAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCCTGCTGTTTGGTGCCGAGGAACGATTTC             <  1:16634/71‑1
                                                             AGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAACGATTTCA            >  1:4070454/1‑71
                                                             AGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGC                                          <  1:2826681/41‑1
                                                                 AAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAACGATTTCACCA         >  1:2184767/1‑70
                                                                  AGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAACGATTTCACCAGC       >  1:3147331/1‑71
                                                                  AGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGT                             <  1:1655053/49‑1
                                                                   GTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTT                                <  1:3235621/45‑1
                                                                     TAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAACGA                 >  1:2609659/1‑58
                                                                     TAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAACGA                 >  1:1646418/1‑58
                                                                     TAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGT                                  <  1:3306950/41‑1
                                                                       GAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGATGAACGATTTCACCAGCGAATC  <  1:3972233/71‑1
                                                                       GAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAACGATTTCACCAGCGAATC  <  1:1747588/71‑1
                                                                       GAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAACGATTTCACCAGCGAAT   <  1:4121711/70‑1
                                                                       GAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTT                                <  1:1298301/41‑1
                                                                                                                                              
TAAACAGGTCCGCCAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/3746841‑3746912
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttagAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAACGATTTCACCAGCGAATC  >  W3110S.gb/3748112‑3748181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.