Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC W3110S.gb 4,310,365 Δ1,199 bp 100% insH[alsK] insH, [alsK]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ W3110S.gb 4310365–4311562 4311563 2–1199 56 [0] [0] 55 insH–[alsK] insH,[alsK]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 43103640 (0.000)39 (0.930) 30/88 NT 100% intergenic (‑286/‑69) yjcS/insH predicted alkyl sulfatase/IS5 element protein
?W3110S.gb 4311564 = 0 (0.000)coding (916/1029 nt) alsK D‑allose kinase

TATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/4310292‑4310364
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttagCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAG  >  W3110S.gb/4311564‑4311632
                                                                                                                                              
TATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTT                                                                          <  1:657276/70‑1
      TTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCA                                                                     >  1:3011744/1‑69
        GTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAG                                                                  <  1:4115000/70‑1
            CTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTG                                                             <  1:751695/71‑1
            CTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTG                                                             >  1:3547962/1‑71
            CTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTG                                                             <  1:2277399/71‑1
              GTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCAC                                                                    >  1:194545/1‑62
                 GTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGC                                                         >  1:517750/1‑70
                   TGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAG                                                                       >  1:3781987/1‑54
                    GGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTG                                                           >  1:2012148/1‑65
                     GCACAGCGGGCCATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAA                                                    <  1:4588317/71‑1
                     GCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAA                                                    <  1:2788666/71‑1
                     GCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAA                                                     <  1:2809480/70‑1
                     GCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAA                                                     <  1:997995/70‑1
                      CACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGG                                                          >  1:2089783/1‑64
                        CAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACG                                                 >  1:2761277/1‑71
                          GCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTT                                               >  1:3117947/1‑71
                          GCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTT                                               <  1:4410016/71‑1
                          GCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAA                                                                >  1:833931/1‑54
                           CGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTT                                               >  1:3456765/1‑70
                            GGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGA                                             <  1:836999/71‑1
                            GGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAG                                                                       >  1:1402738/1‑45
                                  TTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTTGCAAAAAACGTTGATGCGC                                        >  1:222128/1‑70
                                  TTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGT                                                            <  1:1710832/50‑1
                                   TGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCC                                       >  1:1756188/1‑70
                                    GCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAA                                                                >  1:3823887/1‑44
                                    GCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAG                                                                  >  1:97835/1‑42
                                       CTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAA                                                      >  1:2543635/1‑51
                                         ACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACT                                                              <  1:2841217/41‑1
                                          CTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGC                               >  1:2924910/1‑71
                                           TGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGCACTGTGGCAAAAAACGTTGATGCGCCAATATTGCT                              >  1:3283048/1‑71
                                           TGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAA                                                     <  1:4547771/48‑1
                                             TGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGC                            <  1:4602796/71‑1
                                               CCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTG                             >  1:3050273/1‑68
                                               CCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTG                             >  1:2176984/1‑68
                                                   CGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTG                      <  1:3701121/71‑1
                                                    GTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATG                                           >  1:3836406/1‑49
                                                    GTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATG                                           >  1:1127167/1‑49
                                                       TTACTCATGTGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTG                      <  1:3356500/67‑1
                                                       TTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCG                  >  1:4301898/1‑71
                                                        TACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCG                  <  1:2130641/70‑1
                                                        TACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCG                  <  1:1373766/70‑1
                                                         ACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGC                            >  1:270767/1‑59
                                                             ATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGA                                             >  1:1359726/1‑38
                                                              TGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGAAGCGCCAATATTGCTGCACCCTGAGCGCCATTAA           <  1:3221819/71‑1
                                                              TGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTG                                              <  1:4350395/36‑1
                                                                GGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAA                                     <  1:4576798/43‑1
                                                                     TTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCA                                      <  1:601693/37‑1
                                                                      TAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAG     >  1:2672247/1‑69
                                                                      TAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAG     <  1:2144905/69‑1
                                                                      TAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGC                            >  1:376727/1‑46
                                                                      TAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGC                            >  1:921450/1‑46
                                                                       AGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAA   <  1:2120600/70‑1
                                                                       AGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACC                         >  1:1577195/1‑48
                                                                        GCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAG  <  1:4462490/70‑1
                                                                        GCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAG  <  1:768642/70‑1
                                                                        GCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCC                        >  1:2361078/1‑48
                                                                                                                                              
TATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/4310292‑4310364
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttagCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAG  >  W3110S.gb/4311564‑4311632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.