| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,850 | T→G | 25.0% | F373V (TTC→GTC) | thrC → | threonine synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,850 | 0 | T | G | 25.0% | 41.8 / 27.6 | 40 | F373V (TTC→GTC) | thrC | threonine synthase |
| Reads supporting (aligned to +/- strand): ref base T (15/15); new base G (6/4); total (21/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
CGGAGCCGCACGCTGCCGTAGCTTATCGTGCGCTGCGTGATCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCT > W3110S.gb/4782‑4920 | ctgAGCCGCACGCTGCCGTAGCTTATCGTGCTCTGCGTGATCAGTTGAATCCAGGCGAATATGGCTTGt < 1:2363989/67‑1 (MQ=255) ggAGCCGCACGCTGCCGTAGCTTATCGTGCGCTGCGTGATCAGTTGAATCCAGGCGAATATGGCTTGTTc < 1:1543263/70‑1 (MQ=255) ccGTAGCTTATCGTGCGCTGCGTGATCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCAt < 1:1102245/71‑1 (MQ=255) gCTTATCGTGCGCTTCGTGATCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGc > 1:2389867/1‑71 (MQ=255) gCTTATCGTGCGCTGCGTGATCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGc > 1:3489525/1‑71 (MQ=255) gCTTATCGTGCGCTGCGTGATCAGTTGAATCCAGGCGAATATGGCTTGGTCCTCgg > 1:3404354/1‑56 (MQ=255) gCTTATCGTGCGCTGCGTGATCAGTTGAATCCAGGCGAATATGGCTTGGTCCTCgg > 1:4572402/1‑56 (MQ=255) tATCGTGCGCTGCGTGATCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATc > 1:4086254/1‑64 (MQ=255) aTCGTGCGCTGCGTGATCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGaa < 1:2154964/70‑1 (MQ=255) tCGTGCGCTGCGTGATCAGTTGAATCCAGGCGAATATGGCTTGGTCCTCGGCACCGCGCATCCgg > 1:2256919/1‑65 (MQ=255) tCGTGCGCTGCGTGATCAGTTGAATCCAGGCGAATATGGCTTGGTCCTCGGCACCGCGCATCCgg > 1:3034520/1‑65 (MQ=255) gTGCGCTGCGTGATCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGc > 1:2701437/1‑64 (MQ=255) cTGCGTGATCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTa < 1:3246719/67‑1 (MQ=255) cGTGATCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATc < 1:1327608/52‑1 (MQ=255) cGTGATCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAagagag < 1:742495/71‑1 (MQ=255) aTCAGTTGAATCCAGGCGAATATGGCTTGGTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTg < 1:3826775/71‑1 (MQ=255) tCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAAtt > 1:4135130/1‑57 (MQ=255) ttGAATCCAGGCGAATATGGCTTGGTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGc < 1:2513836/71‑1 (MQ=255) gAATCCAGGCGAATATGGCTTGGTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAg < 1:1116254/68‑1 (MQ=255) aTCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGAt > 1:2251480/1‑70 (MQ=255) cAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATtct < 1:3322467/70‑1 (MQ=255) cAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATtct < 1:2073582/70‑1 (MQ=255) aGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCg < 1:2952984/71‑1 (MQ=255) gCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTa < 1:2814279/45‑1 (MQ=255) cGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGa > 1:3187360/1‑62 (MQ=255) gAATATGGCTTGGTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTg > 1:902775/1‑70 (MQ=255) atatGGCTTGGTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGaaa < 1:75809/71‑1 (MQ=255) tatGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTaa < 1:3906844/41‑1 (MQ=255) tatGGCTTGGTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAAc > 1:2750164/1‑71 (MQ=255) tatGGCTTATTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAAc > 1:187923/1‑71 (MQ=255) tGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTa > 1:2255422/1‑38 (MQ=255) tGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGAtt > 1:1303070/1‑58 (MQ=255) tGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCgg > 1:285401/1‑63 (MQ=255) tGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGt > 1:728769/1‑71 (MQ=255) ggCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGtt > 1:180637/1‑71 (MQ=255) ggCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGtt > 1:4318316/1‑71 (MQ=255) cTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTg < 1:1454482/46‑1 (MQ=255) cTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTgg < 1:3260754/71‑1 (MQ=255) cTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGAGAAACGTTgg < 1:932127/71‑1 (MQ=255) ttGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTg < 1:1608035/45‑1 (MQ=255) tGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGa > 1:3817065/1‑70 (MQ=255) ttCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCt < 1:3819219/71‑1 (MQ=255) | CGGAGCCGCACGCTGCCGTAGCTTATCGTGCGCTGCGTGATCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCT > W3110S.gb/4782‑4920 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |