| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,649,687 | T→C | 16.8% | Y14H (TAT→CAT) | dicA → | predicted regulator for DicB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,649,687 | 0 | T | C | 16.8% | 117.1 / 16.2 | 51 | Y14H (TAT→CAT) | dicA | predicted regulator for DicB |
| Reads supporting (aligned to +/- strand): ref base T (18/24); new base C (4/4); total (22/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
TACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTG > W3110S.gb/1649626‑1649752 | tACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGa < 1:755450/71‑1 (MQ=255)tACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGa < 1:2521253/71‑1 (MQ=255) cGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGaaa < 1:1617909/71‑1 (MQ=255) cGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGaa > 1:3532584/1‑70 (MQ=255) cGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGaa > 1:2925816/1‑70 (MQ=255) cGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGaa > 1:2060152/1‑70 (MQ=255) cGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGaa > 1:1967933/1‑70 (MQ=255) gATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGaaaa < 1:903733/71‑1 (MQ=255) gATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGaaaa < 1:2989158/71‑1 (MQ=255) gATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGaaaa < 1:2828864/71‑1 (MQ=255) gATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGAATCGTCGGaaaa < 1:4578152/71‑1 (MQ=255) ttAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAAc > 1:2114444/1‑70 (MQ=255) ttAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAAc > 1:1370379/1‑70 (MQ=255) gCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCa < 1:4108874/71‑1 (MQ=255) aTTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCaaa < 1:1676512/71‑1 (MQ=255) aTTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCaaa < 1:412318/71‑1 (MQ=255) ttAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGt < 1:1381423/50‑1 (MQ=255) ttAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAac < 1:1830106/71‑1 (MQ=255) aGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAaca > 1:3083074/1‑70 (MQ=255) aGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAaca > 1:3323972/1‑70 (MQ=255) aGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAaca < 1:4117716/70‑1 (MQ=255) aaCTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACAcc > 1:2202627/1‑70 (MQ=255) aTGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTAtc < 1:2215617/43‑1 (MQ=255) aTGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTAtc < 1:3179795/43‑1 (MQ=255) aTGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCaa < 1:3453017/69‑1 (MQ=255) aTGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAg > 1:1482540/1‑71 (MQ=255) gAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGaaaaa > 1:802521/1‑50 (MQ=255) gAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGaaaaa > 1:4022156/1‑50 (MQ=255) gAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGt > 1:3114016/1‑70 (MQ=255) gAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGtc < 1:1256996/71‑1 (MQ=255) aaaCAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGtct > 1:3702460/1‑71 (MQ=255) aaaaaTTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTg < 1:521302/70‑1 (MQ=255) aaaTTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTa < 1:4593559/71‑1 (MQ=255) aTTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAg > 1:1840639/1‑58 (MQ=255) aTTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTaaa < 1:527657/71‑1 (MQ=255) tttAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAg < 1:3335671/71‑1 (MQ=255) ttAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAAcc < 1:2006756/40‑1 (MQ=255) tAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCa < 1:1208541/52‑1 (MQ=255) aCTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGccc < 1:316686/70‑1 (MQ=255) aCTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGcc < 1:280839/69‑1 (MQ=255) cTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAac > 1:4622858/1‑42 (MQ=255) cTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAac > 1:2521936/1‑42 (MQ=255) aTCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCt < 1:2031141/60‑1 (MQ=255) tCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAaca > 1:1524920/1‑40 (MQ=255) tCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGa < 1:989484/69‑1 (MQ=255) gCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAAt < 1:1791905/70‑1 (MQ=255) cGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTaaa > 1:683068/1‑54 (MQ=255) cATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCAtg > 1:3131828/1‑71 (MQ=255) cATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCAtg > 1:1197924/1‑71 (MQ=255) aTCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCAtgt > 1:4223354/1‑71 (MQ=255) tCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCAtgtg < 1:2804431/71‑1 (MQ=255) tCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCAtgtg < 1:1089198/71‑1 (MQ=255) | TACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTG > W3110S.gb/1649626‑1649752 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |