| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,693,744 | T→C | 8.9% | E274E (GAA→GAG) | uidC ← | predicted outer membrane porin protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,693,744 | 0 | T | C | 8.9% | 114.4 / 3.3 | 45 | E274E (GAA→GAG) | uidC | predicted outer membrane porin protein |
| Reads supporting (aligned to +/- strand): ref base T (18/23); new base C (2/2); total (20/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGTTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTC > W3110S.gb/1693675‑1693813 | aTAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGttt > 1:3155430/1‑71 (MQ=255) tAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGttt > 1:308379/1‑70 (MQ=255) tAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGttt > 1:995153/1‑70 (MQ=255) tAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGttt > 1:4112004/1‑70 (MQ=255) tAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGTTTc < 1:4181300/71‑1 (MQ=255) accaccACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGTTTCAt < 1:3344954/71‑1 (MQ=255) gCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGTTTCATTGggctggc > 1:1044932/1‑71 (MQ=255) aaaTACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGTTTCAt > 1:2777570/1‑59 (MQ=255) aaaTACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGTTTCATTGGGctggctgg < 1:1679062/71‑1 (MQ=255) aTACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGTCTCATTGGGCTGGCTGGTa > 1:2318218/1‑71 (MQ=255) tACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGTTTc < 1:2969860/54‑1 (MQ=255) tACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGTCTCATTGGGCTGGCTGGTAt < 1:700484/71‑1 (MQ=255) acacTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGTTTCATTg > 1:1147287/1‑57 (MQ=255) cacTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGTTTCAt > 1:4188249/1‑54 (MQ=255) acTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGTTTCATTGGGCTGGCTGGTATTAc > 1:434109/1‑71 (MQ=255) tCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGTTTCATTGGGCTGGCTGGTATTACGAc > 1:409361/1‑71 (MQ=255) ccccACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGTTTCATTGGGCTGGCTGGTATTACGACt > 1:2726008/1‑71 (MQ=255) ccccACGGCGCATTCCAGGT‑AATTGTCCACTAACCAACGCCGTTTCATTGGGCTGGCTGGTATTACGACt < 1:2027555/70‑1 (MQ=255) ccACGGCGCATTCCAGGTCTATTGTCCACTAACCAACGCCGTTTCATTGGGCTGGCTGGTATTACGACTTa < 1:2634445/71‑1 (MQ=255) aCGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGTTTCATTGGGCTGGCTGGTAt < 1:120863/60‑1 (MQ=255) cgcATTCCAGGTCAATTGTCCACTAACCAACGCCGTTTCATTGGGCTGGCTGGTATTACGACTTAAACCt < 1:4032653/70‑1 (MQ=255) ttCCAGGTCAATTGTCCACTAACCAACGCCGTTTCATTGGGCTGGCTGGt < 1:1912574/50‑1 (MQ=255) tCCAGGTCAATTGTCCACTAACCAACGCCGTTTCATTGGGCTGGCTGGTa > 1:1552322/1‑50 (MQ=255) ccAGGTCAATTGTCCACTAACCAACGCCGTTTCAt > 1:2668436/1‑35 (MQ=255) ggTCAATTGTCCACTAACCAACGCCGTTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTgcg > 1:4010282/1‑71 (MQ=255) cAATTGTCCACTAACCAACGCCGTTTCATTGGGCTGGCTGGTa > 1:2008267/1‑43 (MQ=255) cAATTGTCCACTAACCAACGCCGTTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTc > 1:3611514/1‑60 (MQ=255) cAATTGTCCACTAACCAACGCCGTTTAATTGGGctggctgg > 1:3060766/1‑41 (MQ=255) cAATTGTCCACTAACCAACGCCGTCTCATTGGGCTGGCTGGTATTACGACTTAAACCtt > 1:3242969/1‑59 (MQ=255) ttGTCCACTAACCAACGCCGTTTCATTGGGCTGGCTGGt < 1:45168/39‑1 (MQ=255) ttGTCCACTAACCAACGCCGTTTCATTGGGCTGGCTGGt < 1:947561/39‑1 (MQ=255) ttGTCCACTAACCAACGCCGTTTCATTGGGCTGGCTGGTAtt < 1:510280/42‑1 (MQ=255) ttGTCCACTAACCAACGCCGTTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTaaa < 1:4625850/71‑1 (MQ=255) tGTCCACTAACCAACGCCGTTTCATTGGGctggctgg < 1:88591/37‑1 (MQ=255) gTCCACTAACCAACGCCGTTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTaaaa < 1:921913/70‑1 (MQ=255) gTCCACTAACCAACGCCGTTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAg > 1:2052437/1‑71 (MQ=255) cACTAACCAACGCCGTTTCATTGGGCTGGCT‑GTATTACGACTTaa < 1:2174991/45‑1 (MQ=38) cTAACCAACGCCGTTTCATTGGGCTGGCTGGTATTACGACTTaa < 1:1179134/44‑1 (MQ=255) cTAACCAACGCCGTTTCATTGGGCTGGCTGGTATTACGACTTaa < 1:4396884/44‑1 (MQ=255) tAACCAACGCCGTTTCATTGGGCTGGCTGGTATTACGACTTaa < 1:2609429/43‑1 (MQ=255) tAACCAACGCCGTTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGc < 1:3796619/53‑1 (MQ=255) aCCAACGCCGTTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTaa > 1:2707609/1‑60 (MQ=255) aCCAACGCCGTTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGc < 1:3175342/69‑1 (MQ=255) aCCAACGCCGTTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGc < 1:118215/69‑1 (MQ=255) aCCAACGCCGTTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGc < 1:1434296/69‑1 (MQ=255) aCCAACGCCGTTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCaa > 1:1349211/1‑71 (MQ=255) aaCGCCGTCTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTc < 1:3043167/71‑1 (MQ=255) aaCGCCCTTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCg > 1:593640/1‑63 (MQ=255) aCGCCGTTTCATTGGGCTGGCTGGTATTACGACTTaa > 1:3570223/1‑37 (MQ=255) ttCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTc > 1:1487997/1‑70 (MQ=255) | ATAACCACCACTGCCAAATACACTTCCCCACGGCGCATTCCAGGTCAATTGTCCACTAACCAACGCCGTTTCATTGGGCTGGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTC > W3110S.gb/1693675‑1693813 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |