| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,696,825 | T→C | 10.1% | I321V (ATT→GTT) | uidA ← | beta‑D‑glucuronidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,696,825 | 0 | T | C | 10.1% | 186.0 / 11.7 | 69 | I321V (ATT→GTT) | uidA | beta‑D‑glucuronidase |
| Reads supporting (aligned to +/- strand): ref base T (39/23); new base C (2/5); total (41/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01 | |||||||||||
GTTCATCTGCCCAGTCGAGCATCTCTTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTATCGAATCCTTTGCCACGCAAGTCCGCATCT > W3110S.gb/1696756‑1696895 | gTTCATCTGCCCAGTCGAGCATCTCTTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATc > 1:1239529/1‑71 (MQ=255) ttCATCTGCCCAGTCGAGCATCTCTTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATcc < 1:3912498/71‑1 (MQ=255) ttCATCTGCCCAGTCGAGCATCTCTTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATcc < 1:2788034/71‑1 (MQ=255) ttCATCTGCCCAGTCGAGCATCTCTTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATc > 1:45707/1‑70 (MQ=255) ttCATCTGCCCAGTCGAGCATCTCTTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATc < 1:825288/70‑1 (MQ=255) ttCATCTGCCCAGTCGAGCATCTCTTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATc < 1:275421/70‑1 (MQ=255) ttCATCTGCCCAGTCGAGCATCTCTTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATc > 1:311986/1‑70 (MQ=255) ttCATCTGCCCAGTCGAGCATCTCTTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATc > 1:3932937/1‑70 (MQ=255) tCATCTGCCCAGTCGAGCATCTCTTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATc > 1:2203389/1‑69 (MQ=255) cTGCCCAGTCGAGCATCTCTTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTc > 1:826147/1‑70 (MQ=255) cccAGTCGAGCATCTCTTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAACCCAGTc < 1:1234424/67‑1 (MQ=255) cccAGTCGAGCATCTCTTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAACCCAGTc < 1:2521005/67‑1 (MQ=255) cccAGTCGAGCAGCTCTTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAACCCAGTCCAtt < 1:335289/71‑1 (MQ=255) gAGCATCTCTTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCATTaa > 1:4530710/1‑66 (MQ=255) cATCTCTTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTGGt > 1:354998/1‑71 (MQ=255) aTCTCTTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATcc > 1:4612348/1‑52 (MQ=255) tctcTTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCATTa < 1:897849/60‑1 (MQ=255) tctTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCAt > 1:2383480/1‑56 (MQ=255) tctTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCg > 1:1632206/1‑63 (MQ=255) tctTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCACTCCa > 1:369542/1‑55 (MQ=255) ttCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTGGTCGt < 1:234917/68‑1 (MQ=255) ttCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTGGTCGTg < 1:799090/69‑1 (MQ=255) ttCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTGGTCGTg < 1:2698285/69‑1 (MQ=255) ttCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCACAATCCAGTCCATTAATGCGTGGTCGTg < 1:2377080/69‑1 (MQ=255) tCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCg < 1:453290/60‑1 (MQ=255) tCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAACCCAGTCCATTAATg > 1:883988/1‑58 (MQ=255) cAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCa > 1:460028/1‑46 (MQ=255) cAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTGGTCGTGc < 1:1658792/68‑1 (MQ=255) tAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCAtt < 1:1449583/48‑1 (MQ=255) aaGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTGGTCGTGCAc > 1:3324583/1‑64 (MQ=255) aGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCa > 1:3857243/1‑44 (MQ=255) aGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTGGTCGTGCACCATCAGc < 1:1469124/70‑1 (MQ=255) aGGGTAATGCGAGGTACGGTAGGAGTTGG‑CCCAATCCAGTCCATTaa > 1:3864166/1‑47 (MQ=255) ggTAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCAtt < 1:3398088/44‑1 (MQ=255) tAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGtt < 1:3672408/71‑1 (MQ=255) aTGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTa < 1:4293749/70‑1 (MQ=255) aTGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTAt > 1:1023355/1‑71 (MQ=255) aTGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTAt > 1:1547091/1‑71 (MQ=255) tGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTATc > 1:1580553/1‑71 (MQ=255) tGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTATc > 1:2501916/1‑71 (MQ=255) gCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGc > 1:1226418/1‑43 (MQ=255) gCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTAt > 1:1168549/1‑69 (MQ=255) gCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTATc > 1:4279550/1‑70 (MQ=255) cGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTGGTCGTGCACCAt > 1:3588067/1‑57 (MQ=255) cGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTATCg > 1:3605500/1‑70 (MQ=255) gTACGGTAGGAGTTGGCCCCAATCCAGTCCATTaa > 1:4471149/1‑35 (MQ=255) tACGGTAGGAGTTGGCCCCAACCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGt < 1:2033325/60‑1 (MQ=255) aCGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTGGTCGTGCACCATCa > 1:1846238/1‑53 (MQ=255) aCGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCAc > 1:252742/1‑57 (MQ=255) aCGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTATCGAATCCtt > 1:2229687/1‑71 (MQ=255) cGGTAGGAGTTGGCCCCAACCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTATCGAATCCttt < 1:2928594/71‑1 (MQ=255) gTAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTGGTCGTGCAcc > 1:4210225/1‑46 (MQ=255) tAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTGGTCGTGCACCATc < 1:1065054/48‑1 (MQ=255) ggAGTTGGCCCCAATCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTATCGAATCCttt > 1:541161/1‑66 (MQ=255) gTTGGCCCCAATCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTATCGAATc > 1:1727878/1‑59 (MQ=255) ttGGCCCCAATCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTATCGAATCCt < 1:134285/60‑1 (MQ=255) ttGGCCCCAATCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTATCGAATCCTTTGCCACGc < 1:1553963/69‑1 (MQ=255) ttGGCCCCAATCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTATCGAATCCTTTGCCACGc < 1:720041/69‑1 (MQ=255) ttGGCCCCAATCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTATCGAATCCTTTGCCACGc < 1:418945/69‑1 (MQ=255) ggCCCCAATCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTATCGAATCCttt > 1:2142212/1‑60 (MQ=255) ggCCCCAATCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTATCGAATCCTTTGCCACGCAAg > 1:1793277/1‑70 (MQ=255) ggCCCCAATCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTATCGAATCCTTTGCCACGCAAg < 1:1527175/70‑1 (MQ=255) ggCCCCAATCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTATCGAATCCTTTGCCACGCAAg > 1:3579155/1‑70 (MQ=255) cAATCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTATCGAATCCTTTg > 1:4369937/1‑56 (MQ=255) cAATCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTATCGAATCCTTTGCCACGCAAGTCCGCa < 1:2290322/71‑1 (MQ=255) cAACCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTATCGAATCCTTTGCCACGCAAGTCCGc > 1:277437/1‑70 (MQ=255) aaTCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTATCGaa > 1:1726497/1‑48 (MQ=255) aaTCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTATCGaa > 1:1659522/1‑48 (MQ=255) aaTCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTATCGa > 1:1884858/1‑47 (MQ=255) tCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTATCGAATCCTTTGCCAc < 1:3343021/57‑1 (MQ=255) tCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTATCGAATCCTTTGCCACGCAAGTCCGCATCt > 1:2703954/1‑71 (MQ=255) | GTTCATCTGCCCAGTCGAGCATCTCTTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTATCGAATCCTTTGCCACGCAAGTCCGCATCT > W3110S.gb/1696756‑1696895 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |