| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,698,594 | A→G | 10.9% | L58S (TTG→TCG) | uidR ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,698,594 | 0 | A | G | 10.9% | 138.7 / 9.6 | 55 | L58S (TTG→TCG) | uidR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base A (26/23); new base G (3/3); total (29/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01 | |||||||||||
ATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATG > W3110S.gb/1698525‑1698659 | aTCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCaa < 1:4490213/71‑1 (MQ=255)aTCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCaa < 1:2688254/71‑1 (MQ=255) cAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCAAGGcttct > 1:2152440/1‑71 (MQ=255) tCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTg > 1:1337696/1‑71 (MQ=255) cGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCGAGGCTTCTTTg > 1:3715970/1‑70 (MQ=255) ggTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTgg < 1:160655/70‑1 (MQ=255) cccGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGAt > 1:1228170/1‑70 (MQ=255) ccGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCAAGTCTTCTTTGGAGATGa < 1:3718086/71‑1 (MQ=255) ccGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCAAGCCTTCTTTGGAGATGa < 1:1346255/71‑1 (MQ=255) ggAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGaaa > 1:2405459/1‑71 (MQ=255) gAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCGAGGCTTCTTTGGAGATGAAAt > 1:1937544/1‑71 (MQ=255) gAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCGAGGCTTCTTTGGAGATGAAAt < 1:1849088/71‑1 (MQ=255) gAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCAAGGcttc < 1:1057582/57‑1 (MQ=255) gAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCAAGGctt < 1:3177946/56‑1 (MQ=255) aaaCGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATg > 1:4132752/1‑71 (MQ=255) aCGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGt < 1:995936/71‑1 (MQ=255) aCGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGt < 1:3854794/71‑1 (MQ=255) aCGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGt < 1:1738785/71‑1 (MQ=255) aGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATagag < 1:4606806/71‑1 (MQ=255) gcCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGa > 1:2124490/1‑63 (MQ=255) gcCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGa > 1:2685913/1‑63 (MQ=255) tcCTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGa > 1:3546196/1‑49 (MQ=255) tcCTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCgg > 1:4119804/1‑70 (MQ=255) tcCCGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAAt > 1:588740/1‑52 (MQ=255) ccTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGaaa > 1:1224662/1‑50 (MQ=255) cTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATg < 1:1685723/51‑1 (MQ=255) cTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGT‑CCGGGACt < 1:2843119/71‑1 (MQ=255) tGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGt > 1:1091789/1‑52 (MQ=255) ggTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAAt > 1:2339809/1‑48 (MQ=255) ggTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACt > 1:3165051/1‑70 (MQ=255) gTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACt < 1:4053954/69‑1 (MQ=255) cTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGaaa < 1:2959301/43‑1 (MQ=255) cTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGaa < 1:1165649/42‑1 (MQ=255) cTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTcc > 1:3650343/1‑59 (MQ=255) tGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCgg < 1:1548893/61‑1 (MQ=255) gTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAAt > 1:2185564/1‑42 (MQ=255) gTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAAt > 1:2789584/1‑42 (MQ=255) tAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGa < 1:2863959/46‑1 (MQ=255) tAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACt < 1:4330534/63‑1 (MQ=255) tAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACt < 1:1258426/63‑1 (MQ=255) tAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGgcg > 1:524590/1‑70 (MQ=255) aaGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGt < 1:1003116/43‑1 (MQ=255) aGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGAACt < 1:70938/61‑1 (MQ=255) tAATCGCCTGAATCGAGGCTTCTTTGGAGATGaaa > 1:4286455/1‑35 (MQ=255) aaTCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATag > 1:1892000/1‑43 (MQ=255) aTCGCCTGAATCAAGGCTTCTTTGGAGATGAAATg > 1:3603664/1‑35 (MQ=255) tCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTg < 1:1717668/37‑1 (MQ=255) gCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACa < 1:639529/71‑1 (MQ=255) gCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACa < 1:1331407/71‑1 (MQ=255) tGAATCGAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCgg < 1:1316795/46‑1 (MQ=255) tGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGt > 1:1786273/1‑41 (MQ=255) tGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGt > 1:1920304/1‑41 (MQ=255) tGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTa > 1:389192/1‑66 (MQ=255) gAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATg > 1:2498430/1‑71 (MQ=255) aaTCGAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACa < 1:4181310/66‑1 (MQ=255) aTCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTaa > 1:863739/1‑49 (MQ=255) tCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGc > 1:528961/1‑35 (MQ=255) aaGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGa > 1:2458225/1‑42 (MQ=255) | ATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATG > W3110S.gb/1698525‑1698659 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |