| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,713,297 | A→G | 15.4% | T21A (ACC→GCC) | nth → | DNA glycosylase and apyrimidinic (AP) lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,713,297 | 0 | A | G | 15.4% | 125.8 / 15.9 | 52 | T21A (ACC→GCC) | nth | DNA glycosylase and apyrimidinic (AP) lyase |
| Reads supporting (aligned to +/- strand): ref base A (24/20); new base G (4/4); total (28/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.29e-01 | |||||||||||
TCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGATTGCCGTACTGCTTTCCGCTCAGGCGACC > W3110S.gb/1713233‑1713365 | tCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCccac > 1:4187436/1‑66 (MQ=255) cTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCaccacc > 1:3901584/1‑69 (MQ=255) tgatgaATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAg > 1:3625414/1‑71 (MQ=255) atgaATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCt < 1:2480039/71‑1 (MQ=255) tgaATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCt > 1:240823/1‑70 (MQ=255) tAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAAtt > 1:4533889/1‑71 (MQ=255) aaGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAAttt > 1:3293050/1‑70 (MQ=255) cAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAAttt < 1:3305215/67‑1 (MQ=255) aaaaCGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGtt < 1:4630221/71‑1 (MQ=255) aaCGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCg < 1:1712514/71‑1 (MQ=255) tGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCtttt > 1:135047/1‑71 (MQ=255) ggAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAAttt > 1:2721366/1‑57 (MQ=255) gagaTCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCGCCACCGAGCTTAATTTCAGTTCGCCTTTTg > 1:2159544/1‑70 (MQ=255) gagaTCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTc < 1:2184082/57‑1 (MQ=255) gagaTCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTc < 1:4537692/57‑1 (MQ=255) gagaTCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTgt < 1:4298980/71‑2 (MQ=255) gagaTCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTg < 1:4384249/70‑1 (MQ=255) gagaTCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTg < 1:920463/70‑1 (MQ=255) aTCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCGCCACCGAGCTTAATTTCAGTTCGCCTTTTGAAtt < 1:4129614/71‑1 (MQ=255) cTCACTCGCCTGCGTGAGAACAATCCTCATCCCGCCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCt < 1:694475/71‑1 (MQ=255) cTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTg < 1:4389746/64‑1 (MQ=255) aCTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTg < 1:397188/69‑1 (MQ=255) cTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGa < 1:2313742/37‑1 (MQ=255) cTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCg < 1:1718003/53‑1 (MQ=255) tCGCCTGCGTGAGAACAATCCTCATCCCACCACCg > 1:3410599/1‑35 (MQ=255) tCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCt < 1:3652485/66‑1 (MQ=255) gCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTaa > 1:1355263/1‑40 (MQ=255) gCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGa > 1:2311167/1‑66 (MQ=255) gCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGATTGcc > 1:905341/1‑71 (MQ=255) ccTGCGTGAGAACAATCCTCATCCCGCCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGATTGCCg < 1:4514258/71‑1 (MQ=255) tGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGATTGCCGTa > 1:4371109/1‑71 (MQ=255) cGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGATTGCCGTACt > 1:2162677/1‑71 (MQ=255) cGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGATTGCCGTACt > 1:684914/1‑71 (MQ=255) gTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGATTGCCGTACTg > 1:2305726/1‑71 (MQ=255) agaACAATCCTCATCCCGCCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGATTGCCGTACTGCtt < 1:1920954/71‑1 (MQ=255) agaACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGc > 1:3365057/1‑42 (MQ=255) agaACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTg < 1:2953750/48‑1 (MQ=255) agaACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAAt > 1:1959932/1‑51 (MQ=255) gaACAATCCTCATCCCACCACCGAGCTTAATTTCAGtt > 1:1322668/1‑38 (MQ=255) gaACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGc > 1:3778660/1‑41 (MQ=255) gaACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGaa > 1:3452230/1‑49 (MQ=255) gaACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGATTGCCGTACTGCttt < 1:2294114/71‑1 (MQ=255) aaCAATCCTCATCCCACCACCGAGCTTAATTTCAGtt > 1:1743094/1‑37 (MQ=255) aTCCTCATCCCGCCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGATTGCCGTACTGCTTTCCGCt > 1:1097878/1‑71 (MQ=255) aTCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAAt < 1:211329/45‑1 (MQ=255) aTCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGATTGCCGTACTGCTTTCCGCt > 1:3455966/1‑71 (MQ=255) tCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTg < 1:3225344/41‑1 (MQ=255) tCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTg < 1:1353817/41‑1 (MQ=255) ccTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTg > 1:3918474/1‑45 (MQ=255) tCATCCCACCACCGAGCTTAATTTCAGTTCCCCTTTTGAATTGCt < 1:2839547/45‑1 (MQ=255) cATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGa > 1:896474/1‑46 (MQ=255) aTCCCGCCACCGAGCTTAATTTCAGTTCGCCTTTTg > 1:392438/1‑36 (MQ=255) aTCCCGCCACCGAGCTTAATTTCAGTTCGCCTTTTg > 1:4596509/1‑36 (MQ=255) aTCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGATTGCCGTACTGCTTTCCGCTCAGGc > 1:3640503/1‑70 (MQ=255) ccaccaCCGAGCTTAATTTCAGTTCGCCTTTTGAATTGc > 1:1108542/1‑39 (MQ=255) ccaccaCCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGATTGCCGTACTGCTTTCCGCTCAGGCGAcc > 1:847265/1‑71 (MQ=255) accaccGAGCTTAATTTCAGTTCGCCTTTTGAATTGCt < 1:899471/38‑1 (MQ=255) | TCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGATTGCCGTACTGCTTTCCGCTCAGGCGACC > W3110S.gb/1713233‑1713365 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |