| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,779,943 | A→G | 12.3% | E345G (GAG→GGG) | ydiO → | predicted acyl‑CoA dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,779,943 | 0 | A | G | 12.3% | 168.3 / 14.7 | 65 | E345G (GAG→GGG) | ydiO | predicted acyl‑CoA dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (24/33); new base G (3/5); total (27/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01 | |||||||||||
TGCACGTACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAAT > W3110S.gb/1779875‑1780009 | tgCACGTACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAgg > 1:3387293/1‑71 (MQ=255)tgCACGTACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAg < 1:2286936/70‑1 (MQ=255)tgCACGTACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAg < 1:462339/70‑1 (MQ=255)tgCACGTACCGCAATGGAAGTCATTGATGATGCGATTCAAATCAAGGGCGGTCTGGGCTATACCGATGAg < 1:1159278/70‑1 (MQ=255) cGTACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGa > 1:3348511/1‑65 (MQ=255) cGTACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGa > 1:3915033/1‑65 (MQ=255) ccGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGtc > 1:4015996/1‑71 (MQ=255) gCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGa > 1:2015148/1‑59 (MQ=255) cAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTcc > 1:629168/1‑71 (MQ=255) aTGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGa > 1:2449737/1‑56 (MQ=255) aTGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCg > 1:2200091/1‑71 (MQ=255) gAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGGGGCGCGCGTCTCCCGCt > 1:134008/1‑70 (MQ=255) gAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCtt > 1:277791/1‑71 (MQ=255) aaGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCt < 1:641652/69‑1 (MQ=255) aGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGAGCGTCTCCCGCTTCt > 1:1797294/1‑71 (MQ=255) tCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGgcgcgc < 1:1480900/56‑1 (MQ=255) cATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGt > 1:1572200/1‑57 (MQ=255) aTTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGgc < 1:3787983/50‑1 (MQ=255) aTTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCg > 1:2140461/1‑71 (MQ=255) aTTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCg > 1:3860168/1‑71 (MQ=255) ttGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGt < 1:3091603/71‑1 (MQ=255) tgatgaTGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGtct > 1:449874/1‑56 (MQ=255) gatgatGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCTCGTCTCCCGCTTCTGGCGTg < 1:3909912/70‑1 (MQ=255) gatgatGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTg > 1:1913618/1‑65 (MQ=255) gatgatGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTg < 1:1569435/70‑1 (MQ=255) atgatgCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGAt < 1:2483349/71‑1 (MQ=255) atgCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTc < 1:4278136/71‑1 (MQ=255) gCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCg > 1:3235030/1‑71 (MQ=255) cGATTCAAATCATGGGCGGTCTGGGCTATACCGATGGGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCg < 1:1180677/70‑1 (MQ=255) cGATTCAAATCATGGGCGGTCTGGGCTATACCGATGGGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCg < 1:2969516/70‑1 (MQ=255) aTTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGt < 1:1181706/60‑1 (MQ=255) aTTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTg < 1:3857723/61‑1 (MQ=255) ttCAAATCATGGGCGGTCTGGGCTATACCGATGGGgcgcgc < 1:943514/41‑1 (MQ=255) tCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGt < 1:2833321/58‑1 (MQ=255) tCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGt < 1:3451479/58‑1 (MQ=255) cAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTtgtg < 1:4208167/70‑1 (MQ=255) aaaTCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGa < 1:997534/70‑1 (MQ=255) aaTCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTgg < 1:520106/52‑1 (MQ=255) aaTCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGt < 1:2209411/55‑1 (MQ=255) aTCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACg < 1:3858066/71‑1 (MQ=255) aTCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACg < 1:2888664/71‑1 (MQ=255) tCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGt < 1:2325760/71‑1 (MQ=255) aTGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGt > 1:2606198/1‑69 (MQ=255) tGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGt < 1:2048461/59‑1 (MQ=255) gCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATcggc > 1:3584916/1‑71 (MQ=255) gCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATcggc < 1:3164341/71‑1 (MQ=255) gTCTGGGCTATACCGATGGGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTAt < 1:444093/64‑1 (MQ=255) gTCTGGGCTATACCGATGGGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTAt < 1:3289179/64‑1 (MQ=255) gTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCAGTTGTGAACGTATcggcgg > 1:4285042/1‑70 (MQ=255) tCTGGGCTATACCGATGGGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATcggcggcg > 1:3041994/1‑71 (MQ=255) tGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTg < 1:3364966/42‑1 (MQ=255) ggCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGa > 1:3206060/1‑41 (MQ=255) ggCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATcggcggcgt < 1:3725478/68‑2 (MQ=255) ggCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATcggcggcgg < 1:4503985/68‑1 (MQ=255) ggCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTa < 1:1578075/70‑1 (MQ=255) gCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACa < 1:3731514/71‑1 (MQ=255) tataCCGATGATGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTtgtg < 1:2888180/49‑1 (MQ=255) tataCCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGa > 1:2749300/1‑71 (MQ=255) tataCCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGa > 1:1606911/1‑71 (MQ=255) tataCCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGa > 1:4517763/1‑71 (MQ=255) taCCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTg < 1:952624/35‑1 (MQ=255) taCCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATcgg > 1:2901124/1‑57 (MQ=255) ccGATGGGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGa > 1:342582/1‑70 (MQ=255) ccGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTAt > 1:339977/1‑52 (MQ=255) ccGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATcggcggcgg > 1:2181559/1‑61 (MQ=255) ccGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTa > 1:4642778/1‑63 (MQ=255) ccGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTa > 1:256835/1‑63 (MQ=255) gATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACg < 1:519740/47‑1 (MQ=255) gATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAAt < 1:953282/71‑1 (MQ=255) | TGCACGTACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAAT > W3110S.gb/1779875‑1780009 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |