| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,796,665 | C→T | 27.3% | A68T (GCG→ACG) | btuC ← | vitamin B12 transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,796,665 | 0 | C | T | 27.3% | 53.9 / 18.7 | 33 | A68T (GCG→ACG) | btuC | vitamin B12 transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (12/12); new base T (7/2); total (19/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTCCAGGTTCTGCCAGAGGATTTTCAAACAACGCCTGCATTACAGCGCCGGATATAGCCAGCGCCGCACCAACCAGCAATACAGCCAGCGTACGTGGCAGGCGAATTTGCCAGACGAACAGTTCGCCACGAG > W3110S.gb/1796599‑1796730 | gtCCAGGTTCTGCCAGAGGATTTTCAAACAACGCCTGCATTACAGCGCCGGATATAGCCAGCGCCGCacc > 1:4361151/1‑70 (MQ=255) ggTTCTGCCAGAGGATTTTCAAACAACGCCTGCATTACAGCGCCGGATATAGCCAGCGCCGCaccaac > 1:1371057/1‑68 (MQ=255) gTTCTGCCAGAGGATTTTCAAACAACGCCTGCATTACAGCGCCGGATATAGCCAGCGCCGCACCAACCAGc < 1:1892625/71‑1 (MQ=255) tCTGCCATAGGATTTTCAAACAACGCCTGCATTACAGCGCCGGATATAGCCAGCGCCGTACCAACCAGCaa > 1:630989/1‑71 (MQ=255) tGCCAGAGGATTTTCAAACAACGCCTGCATTACAGCGCCGGATATAGCCAGCGCCGTACCAACCAGCaa < 1:2731044/69‑1 (MQ=255) ccAGAGGATTTTCAAACAACGCCTGCATTACAGCGCCGGATATAGCCAGCGCCGTACCAACCAGCAATa > 1:1143835/1‑69 (MQ=255) agGATTTTCAAACAACGCCTGCATTACAGCGCCGGATATAGCCAGCGCCGTACCAACCAGCAATAcagc > 1:3829454/1‑69 (MQ=255) gATTTTCAAACAACGCCTGCATTACAGCGCCGGATATAGCCAGCGCCGCACCAACCAGCAATAcagccag < 1:3542069/70‑1 (MQ=255) gATTTTCAAACAACGCCTGCATTACAGCGCCGGATATAGCCAGCGCCGCACCAACCAGCAATAcagcc > 1:3303258/1‑68 (MQ=255) gATTTTAAAACAACGCCTGCATTACAGCGCCGGATATAGCCAGCGCCGCa > 1:1157004/1‑50 (MQ=255) tttCAAACAACGCCTGCATTACAGCGCCGGATATAGCCAGCGCCGCACCAACCAg < 1:4510309/55‑1 (MQ=255) cAAACAACGCCTGCATTACAGCGCCGGATATAGCCAGCGCCGCACCAACCAGCAATACAGCCAGCGTACGt > 1:480804/1‑71 (MQ=255) caacGCCTGCATTACAGCGCCGGATATAGCCAGCGCCGCACCAACCAGCAATACAGCCAGCGTACGTGGCa < 1:413018/71‑1 (MQ=255) aacGCCTGCATTACAGCGCCGGATATAGCCAGCGCCGCACCAACCAGCAAt < 1:4263194/51‑1 (MQ=255) tGCATTACAGCGCCGGATATAGCCAGCGCCGCACCAACCAGCAAt < 1:4635761/45‑1 (MQ=255) cATTACAGCGCCGGATATAGCCAGCGCCGCACCAACCAGCAATACAGCCAGCGTACGTGGc < 1:4230998/61‑1 (MQ=255) cATTACAGCGCCGGATATAGCCAGCGCCGCACCAACCAGCAATACAGCCAGCGTACGTGGc < 1:4532879/61‑1 (MQ=255) cATTACAGCGCCGGATATAGCCAGCGCCGCACCAACCAGCAATACAGCCAGCGTACGTGGCAGGCGAAttt > 1:4037133/1‑71 (MQ=255) aTTACAGCGCCGGATATAGCCAGCGCCGCACCAACCAGCAATACAGCCAGCGTACGTGGCAGGCGAATTTg < 1:234762/71‑1 (MQ=255) ttACAGCGCCGGATATAGCCAGCGCCGCACCAACCAGCaa < 1:184977/40‑1 (MQ=255) aCAGCGCCGGATATAGCCAGCGCCGTACCAACCAGCAATACAGCCAGCGTAc > 1:3027331/1‑52 (MQ=255) aCAGCGCCGGATATAGCCAGCGCCGCACCAACCAGCAATACAGCCAGCGTACGTGGCAGGCGAAt > 1:3722317/1‑65 (MQ=255) aCAGCGCCGGATATAGCCAGCGCCGCACCAACCAGCAATACAGCCAGCGTACGTGGCAGGCGAATTTGc > 1:2767110/1‑69 (MQ=255) aCAGCGCCGGATATAGCCAGCGCCGCACCAACCAGCAATACAGCCAGCGTACGTGGCAGGCGAATTTGc > 1:3339057/1‑69 (MQ=255) cAGCGCCGGATATAGCCAGCGCCGCACCAACCAGCAATACAGCCAGCGTACGTGGCAGGCGAATTTGc > 1:2488466/1‑68 (MQ=255) cAGCGCCGGATATAGCCAGCGCCGCACCAACCAGCAATACAGCCAGCGTACGTGGCAGGCGAATTTGc > 1:2493906/1‑68 (MQ=255) gcCGGATATAGCCAGCGCCGTACCAACCAGCAATACAGCCAGCGTACGTGGCAGGCGAATTTGCCAGACg < 1:4242566/70‑1 (MQ=255) atatAGCCAGCGCCGTACCAACCAGCAATACAGCCAGCGTa > 1:1341064/1‑41 (MQ=255) atatAGCCAGCGCCGCACCAACCAGCAATACAGCCAGCGTACGTGGCAGGCGAATTTGCCAGACGaa < 1:1939828/67‑1 (MQ=255) tataGCCAGCGCCGCACCAACCAGCAATACAGCCAGCGTACGTGGCAGGCGAATTTGCCAGACGAACAGtt > 1:3977366/1‑71 (MQ=255) ataGCCAGCGCCGTACCAACCAGCAATACAGCCAGCGTACGTGGCAGGCGAATTTGCCAGACGAACAGTTc > 1:1979565/1‑71 (MQ=255) ccAGCGCCGTACCAACCAGCAATACAGCCAGCGTACGTGGCAGGCGAATTTGCCAGAc > 1:1607407/1‑58 (MQ=255) gcgcCGCACCAACCAGCAATACAGCCAGCGTAAGTGGCAGGCGaa < 1:3751498/45‑1 (MQ=255) cgccgtACCAACCAGCAATACAGCCAGCGTACGTGGCAGGCGAATTTGCCAGACGAACAGTTCGCCACgag < 1:2405828/71‑1 (MQ=255) cgcACCAACCAGCAATACAGCCAGCGTACGTGGCAGGCGAATTTGCCAGACGAACAGt > 1:3402438/1‑58 (MQ=255) cgcACCAACCAGCAATACAGCCAGCGTACGTGGCAGGCGAATTTGCCAGACGAACAGt > 1:607599/1‑58 (MQ=255) | GTCCAGGTTCTGCCAGAGGATTTTCAAACAACGCCTGCATTACAGCGCCGGATATAGCCAGCGCCGCACCAACCAGCAATACAGCCAGCGTACGTGGCAGGCGAATTTGCCAGACGAACAGTTCGCCACGAG > W3110S.gb/1796599‑1796730 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |