| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 71,175 | A→G | 91.8% | R263R (CGA→CGG) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 71,175 | 0 | A | G | 91.8% | 1343.3 / 72.7 | 429 | R263R (CGA→CGG) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base A (16/19); new base G (189/203); total (206/223) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.31e-01 | |||||||||||
Only 100 of 430 total aligned reads displayed.
ACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATG > W3110S.gb/71107‑71245 | accaCCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGt < 1:2627857/68‑1 (MQ=255)aCTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAg < 1:1688364/70‑1 (MQ=255) ccaCCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTa < 1:1744628/69‑1 (MQ=255) aCCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTAtt > 1:26987/1‑71 (MQ=255) gATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaa < 1:4511143/71‑1 (MQ=255) aTGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaaa < 1:364735/71‑1 (MQ=255) tGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGt < 1:3819490/61‑1 (MQ=255) tGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaaaa < 1:2924078/71‑1 (MQ=255) tGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaaaa < 1:3881382/71‑1 (MQ=255) gCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaa < 1:608230/68‑1 (MQ=255) ccTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATg < 1:4263323/71‑1 (MQ=255) ccTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATg < 1:4288569/71‑1 (MQ=255) cTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATg > 1:2514077/1‑70 (MQ=255) cTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGc < 1:3562663/71‑1 (MQ=255) cTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGc < 1:1647821/71‑1 (MQ=255) tATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTAt < 1:3114813/59‑1 (MQ=255) aTCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTAGCGAGTATTTAAAAAATGCAc > 1:4411113/1‑71 (MQ=255) tCGCCACCGTCTGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCAc < 1:1875824/70‑1 (MQ=255) tCGCCACCGTCGGTCGCAATGTTGGTTTTGACGGTCAACTCTATTTCTCGCGGGTATTTAAAAAATGCa < 1:4155725/69‑1 (MQ=255) tCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaaaa < 1:4570627/65‑1 (MQ=255) tCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCAcc < 1:1457524/71‑1 (MQ=255) tCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCAcc > 1:2572337/1‑71 (MQ=255) cGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaa > 1:1043077/1‑61 (MQ=255) cGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCg > 1:1409386/1‑71 (MQ=255) gCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaaaa > 1:847193/1‑63 (MQ=255) gCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCAc > 1:4352058/1‑68 (MQ=255) gCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCgg > 1:1847678/1‑71 (MQ=255) gCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCgg > 1:4028995/1‑71 (MQ=255) ccACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaa > 1:4434578/1‑58 (MQ=255) ccACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCggg > 1:2244426/1‑71 (MQ=255) cACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATg < 1:3478279/63‑1 (MQ=255) aCCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGc < 1:266655/71‑1 (MQ=255) aCCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGc > 1:1474124/1‑71 (MQ=255) ccGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCa > 1:3137583/1‑63 (MQ=255) ccGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTaaaa < 1:3996365/57‑1 (MQ=255) cGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaa < 1:3073835/56‑1 (MQ=255) cGTCGGTCGCAATGTTGGGTTTGACGATCAACTCTATTTCTCGCGAGTAt < 1:896970/50‑1 (MQ=255) gtcggtcgCAATGTTGGTTTTGAGGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAg < 1:3746644/71‑1 (MQ=255) gtcggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAAt > 1:4143979/1‑58 (MQ=255) gtcggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCa > 1:1957587/1‑61 (MQ=255) gtcggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCa > 1:4082712/1‑61 (MQ=255) gtcggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCgggg > 1:1000955/1‑67 (MQ=255) gtcggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAg > 1:3909740/1‑71 (MQ=255) gtcggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAg < 1:2314419/71‑1 (MQ=255) gtcggtcgCAATGTCGGTTTTGACGCTCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAg > 1:2337811/1‑71 (MQ=255) tcggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaa < 1:757912/54‑1 (MQ=255) ggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTa > 1:2710443/1‑45 (MQ=255) ggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGccc > 1:1186741/1‑71 (MQ=255) cgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaa < 1:3368316/49‑1 (MQ=255) cgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcgag > 1:3968781/1‑71 (MQ=255) gCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcgagc < 1:479395/71‑1 (MQ=255) gCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcgagc < 1:3710754/71‑1 (MQ=255) gCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcgagc < 1:3023758/71‑1 (MQ=255) gCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCcgagc > 1:217062/1‑71 (MQ=255) cAATGTTGGTTTTGACTATCAACTCTATTTCTCGCGTGTATTTAAAAAATGCAcc < 1:936715/55‑1 (MQ=255) cAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcgagcg < 1:2102374/71‑1 (MQ=255) cAATGTGGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcgagcg < 1:3942245/71‑1 (MQ=255) aaTGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCa > 1:647070/1‑52 (MQ=255) aaTGTTGGTTTTGACGATCAACTCTATTTATCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcgagc < 1:2762187/69‑1 (MQ=255) aTGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATg < 1:1915927/49‑1 (MQ=255) aTGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGcc > 1:203258/1‑63 (MQ=255) aTGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcgagcgag < 1:1381765/71‑1 (MQ=255) tGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaa < 1:4610940/44‑1 (MQ=255) tGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGt < 1:1531572/71‑1 (MQ=255) gTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCAc > 1:1085402/1‑50 (MQ=255) gTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcg < 1:1732461/63‑1 (MQ=255) gTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATg < 1:3244107/47‑1 (MQ=255) ttGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCgg > 1:1795752/1‑52 (MQ=255) ttGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGttc < 1:1503274/71‑2 (MQ=255) gTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaa < 1:3180988/38‑1 (MQ=255) gTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaa < 1:295016/38‑1 (MQ=255) ttttGACGATCAACTCTATTTCTCGCGGGTATTTaaaa < 1:2832351/38‑1 (MQ=255) ttGACGATCAACTCTATTTCTCGCGGGTATTTaaaaaa > 1:1133944/1‑38 (MQ=255) ttGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGcc > 1:1595755/1‑71 (MQ=255) ttGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGcc > 1:657286/1‑71 (MQ=255) ttGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGc > 1:4462703/1‑49 (MQ=255) gACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCAc > 1:1221378/1‑41 (MQ=255) aCGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGt < 1:1281370/71‑1 (MQ=255) aCGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGt < 1:2131565/71‑1 (MQ=255) gATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcg < 1:1333124/51‑1 (MQ=255) tCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGtt < 1:3625557/57‑1 (MQ=255) cAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcgagcgag > 1:2451435/1‑54 (MQ=255) cAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcga > 1:2552053/1‑49 (MQ=255) cAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTtgtg > 1:2607891/1‑70 (MQ=255) cAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTtgtg > 1:3784193/1‑70 (MQ=255) cAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTtgtg > 1:2398079/1‑70 (MQ=255) cAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTtgtg > 1:3444304/1‑70 (MQ=255) cAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTtgtg > 1:219960/1‑70 (MQ=255) aaCTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTtgtg > 1:2800431/1‑69 (MQ=255) ctATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTgaagaa > 1:793056/1‑70 (MQ=255) tATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGcc < 1:3685756/57‑1 (MQ=255) aTTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaa < 1:329269/69‑1 (MQ=255) tttCTCGCTGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaa < 1:4312028/68‑1 (MQ=255) ttCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGttc < 1:51438/48‑2 (MQ=255) tctcGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAg > 1:721757/1‑69 (MQ=255) tctcGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGt > 1:593082/1‑70 (MQ=255) tcGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGaa > 1:4635713/1‑71 (MQ=255) gcgGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAatg < 1:2967626/71‑1 (MQ=255) gcgGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAat < 1:1575346/70‑1 (MQ=255) gcgAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAat < 1:2463412/70‑1 (MQ=255) | ACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATG > W3110S.gb/71107‑71245 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |