| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,800,301 | A→C | 10.5% | F119C (TTC→TGC) | pheS ← | phenylalanine tRNA synthetase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,800,301 | 0 | A | C | 10.5% | 126.3 / 13.1 | 57 | F119C (TTC→TGC) | pheS | phenylalanine tRNA synthetase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base A (19/32); new base C (4/2); total (23/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
GCATCGAAGTTATGATAATCGTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAGACCGCCGTTTTCAATGCGACGAC > W3110S.gb/1800232‑1800364 | gCATCGAAGTTATGATAATCGTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCgaagaa < 1:2348358/71‑1 (MQ=255) cATCGAAGTTATGATAATCGTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGaaa > 1:2453857/1‑71 (MQ=255) aTCGAAGTTATGATAATCGTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCgaaga < 1:3577348/68‑1 (MQ=255) tCGAAGTTATGATAATCGTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACt < 1:364179/71‑1 (MQ=255) tCGAAGTTATGATAATCGTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACt > 1:3414212/1‑71 (MQ=255) gAAGTTATGATAATCGTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACttt > 1:1926380/1‑71 (MQ=255) gAAGTTATGATAATCGTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACtt < 1:1634696/70‑1 (MQ=255) gAAGTTATGATAATCGTCTTCGATTTCCGGCCCGGTTGCCAAGGTAAAGCCAAGCTCACCGAAGAAACttt > 1:49380/1‑71 (MQ=255) aaGTTATGATAATCGTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTc < 1:4115845/71‑1 (MQ=255) aaGTTATGATAATCGTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTc < 1:4499393/71‑1 (MQ=255) aaGTTATGATAATCGTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTc < 1:676104/71‑1 (MQ=255) gTTATGATAATCGTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGCAACTTTCGa > 1:4334883/1‑71 (MQ=255) aTGATAATCGTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATa < 1:2287875/70‑1 (MQ=255) tGATAATCGTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACt < 1:4418985/62‑1 (MQ=255) tAATCGTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTc > 1:3912586/1‑71 (MQ=255) tAATCGTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACtt > 1:838487/1‑60 (MQ=255) tAATCGTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTc < 1:2747328/71‑1 (MQ=255) cGTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATg > 1:1132132/1‑71 (MQ=255) gTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGaaa > 1:2258634/1‑52 (MQ=255) gTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATgg < 1:3727527/71‑1 (MQ=255) gTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATgg < 1:925510/71‑1 (MQ=255) tCTTCGATTTCCGGCCCGGTTGCCACGTTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATgg < 1:2738948/70‑1 (MQ=255) ttCGATTTCCGGCCCGGTTGCGACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGAt < 1:4143938/66‑1 (MQ=255) ttCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCgaaga < 1:2739846/47‑1 (MQ=255) ttCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTc < 1:449149/54‑1 (MQ=255) cGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATGGTACgg > 1:1846500/1‑71 (MQ=255) tttCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCgaagaa < 1:932580/43‑1 (MQ=255) tttCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATGGTACGGGTa < 1:1384024/71‑1 (MQ=255) tttCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATGGTACGGGTa < 1:3599066/71‑1 (MQ=255) tCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATa > 1:185011/1‑51 (MQ=255) cGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATGGTACTGGTAACCGGATg > 1:156805/1‑70 (MQ=255) ggTTGCCACGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCGATGGTACGGGTAACCGGATg > 1:1142339/1‑69 (MQ=255) ggTTGCCACGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCGATGATACGGGTAACCGGa < 1:373296/67‑1 (MQ=255) gTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTc < 1:1514089/38‑1 (MQ=255) gTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATGGTACGGGTAAc < 1:2301036/62‑1 (MQ=255) gTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATGGTACGGGTAACCGGATg > 1:4241357/1‑68 (MQ=255) gTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAg < 1:4587480/71‑1 (MQ=255) ttGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTc < 1:3018093/37‑1 (MQ=255) tGCCACGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAGAc > 1:4550352/1‑71 (MQ=255) tGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATGGTa > 1:2122082/1‑52 (MQ=255) tGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAGa > 1:636219/1‑70 (MQ=255) tGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAGAc > 1:185441/1‑71 (MQ=255) gCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAGAcc > 1:4457417/1‑71 (MQ=255) ccACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATgg < 1:3643679/48‑1 (MQ=255) cACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATGGTACgg > 1:990776/1‑52 (MQ=255) aCGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAGAccgcc < 1:3552261/71‑1 (MQ=255) cGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATa > 1:3084624/1‑35 (MQ=255) gTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGa < 1:1114995/40‑1 (MQ=255) gTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGa < 1:1997832/40‑1 (MQ=255) gTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAGACCGCCGtt < 1:1985603/71‑1 (MQ=255) aaaGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAGACCGCCGttt > 1:3685670/1‑70 (MQ=255) aaGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATGGTACGGGTAACCGGATg < 1:4440401/56‑1 (MQ=255) aaGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAGACCGCCGTTTTc < 1:3195748/71‑1 (MQ=255) aaGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAGACCGCCGTTTTc < 1:2579813/71‑1 (MQ=255) gCCAAGCTCACCGAAGCAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAGACCGCCGtttt < 1:2394023/68‑1 (MQ=255) gCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGc < 1:1368271/55‑1 (MQ=255) aaGCTCACCGAAGAAACTTTCGATACGGTCGATGGTACGGGTAACCgg > 1:1209968/1‑48 (MQ=255) aaGCTCACCGAAGAAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAGACCGCCGTTTTCAATGc < 1:3083473/71‑1 (MQ=255) tCACCGAAGAAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAGACCGCCGTTTTCAATGcgac > 1:3641884/1‑70 (MQ=255) tCACCGAAGAAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAGACCGCCGTTTTCAATGcgac > 1:376401/1‑70 (MQ=255) aCCGAAGAAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAGACCGCCGTTTTCAATGcgacg < 1:597000/69‑1 (MQ=255) aCCGAAGAAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAGACCGCCGTTTTCAATGcgacg < 1:1469881/69‑1 (MQ=255) aCCGAAGAAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAGACCGCCGTTTTCAATGCgacgac < 1:1911817/71‑1 (MQ=255) gaagaaACTTTCGATACGGTCGATGGTACGGGTaa > 1:3210134/1‑35 (MQ=255) gaagaaACTTTCGATACGGTCGATGGTACGGGTAACCGGa > 1:2783119/1‑40 (MQ=255) aaaCTTTCGATACGGTCGATGGTACGGGTAACCGGATGCa > 1:4634149/1‑40 (MQ=255) | GCATCGAAGTTATGATAATCGTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAGACCGCCGTTTTCAATGCGACGAC > W3110S.gb/1800232‑1800364 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |