| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,803,912 | G→T | 18.3% | H125N (CAT→AAT) | thrS ← | threonyl‑tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,803,912 | 0 | G | T | 18.3% | 141.4 / 12.5 | 49 | H125N (CAT→AAT) | thrS | threonyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base G (18/22); new base T (5/4); total (23/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.16e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 | |||||||||||
AGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAA > W3110S.gb/1803844‑1803980 | aGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCa > 1:1331867/1‑71 (MQ=255)aGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCa > 1:682676/1‑71 (MQ=255)aGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCa > 1:4180337/1‑71 (MQ=255)aGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCa < 1:2246958/71‑1 (MQ=255)aGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCa > 1:2246826/1‑71 (MQ=255)aGTTTCACGCGCTTCGTGCCAGCTGACTTT‑TTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCa > 1:553741/1‑70 (MQ=255) tttCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATTCATc > 1:321243/1‑71 (MQ=255) tCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATcc < 1:2141857/70‑1 (MQ=255) aCGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGc < 1:1571826/70‑1 (MQ=255) gcgcTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTtc < 1:509741/71‑1 (MQ=255) gcTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATTCATCCGCTtctc < 1:144276/71‑1 (MQ=255) ccAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCtt < 1:4067506/71‑1 (MQ=255) gCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGa < 1:639469/71‑1 (MQ=255) gACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACAt > 1:4046100/1‑71 (MQ=255) tttCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTc < 1:4168538/63‑1 (MQ=255) ttcttcTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCt < 1:2298911/49‑1 (MQ=255) tcttctTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGa > 1:4517498/1‑63 (MQ=255) cttcttAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATc > 1:318803/1‑43 (MQ=255) ttcttAATGACGTCGTAGTTTTTCTCAGCAAGCTCATTCATCCGCTTCTCGAGTGCTTCGACATCTTCCTg > 1:211989/1‑71 (MQ=255) ttcttAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTg > 1:989255/1‑71 (MQ=255) tcttAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTtctc < 1:1464693/49‑1 (MQ=255) tAATGACGTCGTAGTTTTTCTCAGCAAGCTCATTCATCCGCTTCTCGAGTGCTTCGa > 1:436137/1‑57 (MQ=255) aTGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTaa < 1:3553073/71‑1 (MQ=255) tcgtAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTa < 1:855545/64‑1 (MQ=255) cgtAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAg > 1:747592/1‑40 (MQ=255) gtAGTTTTTCTCAGCAAGCTCATTCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGtt < 1:2067621/61‑1 (MQ=255) gtAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAg > 1:4607022/1‑39 (MQ=255) gtAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTaa > 1:4176329/1‑63 (MQ=255) gtAGTTTTTCTCAGCAAGCTCATGCATCAGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGt > 1:2128649/1‑71 (MQ=255) gTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGAc < 1:2766693/45‑1 (MQ=255) tttttCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACg < 1:2747707/61‑1 (MQ=255) ttttCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTc < 1:735694/40‑1 (MQ=255) tttCTCAGCAAGCTCATTCATCCGCTTCTCGAGTGc > 1:3061030/1‑36 (MQ=255) tttCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTc > 1:1505470/1‑66 (MQ=255) tttCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGa < 1:2922245/70‑1 (MQ=255) ttCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTc > 1:4235890/1‑47 (MQ=255) tctcAGCAAGCTCATTCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATc > 1:1756448/1‑70 (MQ=255) tctcAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATc < 1:3807153/70‑1 (MQ=255) aGCAAGCTCATTCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAAcgt < 1:4036902/71‑1 (MQ=255) aGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCaa < 1:4575031/68‑1 (MQ=255) cAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCTGTCAAGATCAAc < 1:2323417/67‑1 (MQ=255) aaGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTc > 1:4116110/1‑39 (MQ=255) aGCTCATTCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGtt < 1:2928442/45‑1 (MQ=255) gCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGtaa < 1:3324213/71‑1 (MQ=255) tCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGtt < 1:2744236/42‑1 (MQ=255) tCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGtaat > 1:3550249/1‑70 (MQ=255) tCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGtaa > 1:3644525/1‑69 (MQ=255) aTGCATCCGCTTCTAGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATaaa < 1:3733765/71‑1 (MQ=255) tGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTg < 1:3861454/35‑1 (MQ=255) | AGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAA > W3110S.gb/1803844‑1803980 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |