| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,804,377 | A→G | 22.4% | intergenic (‑93/‑431) | thrS ← / → arpB | threonyl‑tRNA synthetase/ECK1718:JW5278:b1720; hypothetical protein, N‑ter fragment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,804,377 | 0 | A | G | 22.4% | 126.9 / 37.6 | 67 | intergenic (‑93/‑431) | thrS/arpB | threonyl‑tRNA synthetase/ECK1718:JW5278:b1720; hypothetical protein, N‑ter fragment |
| Reads supporting (aligned to +/- strand): ref base A (27/25); new base G (6/9); total (33/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01 | |||||||||||
TGACCCACACGAAAGATCACATACAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACATTAACCTCTCACCTTCCACCC > W3110S.gb/1804307‑1804443 | tgACCCACACGAAAGATCACATACAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCca < 1:4350206/71‑1 (MQ=255) aCCCACACGAAAGATCACATACAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCcaa > 1:4183378/1‑70 (MQ=255) cacaCGAAAGATCACATACAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCcgaca < 1:178241/69‑1 (MQ=255) cacaCGAAAGATCACATACAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCGACaaa < 1:4010534/71‑1 (MQ=255) cacaCGAAAGATCACATACAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACaaa < 1:2864778/71‑1 (MQ=255) acGAAAGATCACATACAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACaaa > 1:709804/1‑68 (MQ=255) acGAAAGATCACATACAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATg > 1:2841197/1‑70 (MQ=255) acGAAAGATCACATACAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGc > 1:4607323/1‑71 (MQ=255) cGAAAGATCACATACAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCGACAAAt > 1:133062/1‑68 (MQ=255) gAAAGATCACATACAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTa < 1:4487511/71‑1 (MQ=255) gAAAGATCACATACAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTa < 1:4136417/71‑1 (MQ=255) aTCACATACAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTg < 1:2573190/71‑1 (MQ=255) aTCACATACAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTg > 1:2144990/1‑71 (MQ=255) cacaTACAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTa > 1:3389724/1‑64 (MQ=255) acaTACAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGtt > 1:4121265/1‑71 (MQ=255) acaTACAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGtt > 1:1949861/1‑71 (MQ=255) caTACAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCGACAAATGCTAAATTGGTTc > 1:3647894/1‑71 (MQ=255) tACAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGc < 1:1450429/71‑1 (MQ=255) aCAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTaaa > 1:3151914/1‑61 (MQ=255) cAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCGACAAATGCTAAATTGGTTCGCaa < 1:4186584/71‑1 (MQ=255) cAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCaa > 1:54522/1‑71 (MQ=255) cAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCaa > 1:1197501/1‑71 (MQ=255) aaaGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCaa < 1:1839112/70‑1 (MQ=255) aaaaaTTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAgg > 1:1976031/1‑71 (MQ=255) aaaaaTTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAgg < 1:3448375/71‑1 (MQ=255) aaaaaTTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAgg > 1:3371417/1‑71 (MQ=255) aaaaTTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCGACAAATGCTAAATTgg > 1:2306738/1‑58 (MQ=255) aaaTTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTg < 1:1912594/56‑1 (MQ=255) aaaTTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAAt < 1:675321/65‑1 (MQ=255) aTTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCGACAAATGCTAAATTGGt < 1:3667772/56‑1 (MQ=255) aTTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTaa < 1:3763020/50‑1 (MQ=255) aTTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTaa < 1:1054884/50‑1 (MQ=255) aTTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTaca > 1:985514/1‑71 (MQ=255) aTTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTaca > 1:2664812/1‑71 (MQ=255) aTTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTac < 1:1795953/70‑1 (MQ=255) tttGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTaca > 1:239588/1‑70 (MQ=255) tttGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTaca > 1:522334/1‑70 (MQ=255) tattatCAGTTCATTGCGAAACCATCTAGCCGACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATg > 1:695871/1‑70 (MQ=255) attaACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGa > 1:4198848/1‑70 (MQ=255) attaACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGa < 1:1330168/70‑1 (MQ=255) ttaACAGTTCATTGCGAAACCATCTAGCCAACAAATCCTAAATTGGTTCGCAATCAGGTACACAAATGaaa < 1:1201985/71‑1 (MQ=255) taACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGaaa < 1:2993258/70‑1 (MQ=255) taACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGa > 1:4476215/1‑68 (MQ=255) aaCAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGa > 1:1822111/1‑71 (MQ=255) aCAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAg < 1:3877008/69‑1 (MQ=255) gTTCATTGCGAAACCATCTAGCCGACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACAtt < 1:2425666/71‑1 (MQ=255) gTTCATTGCGAAACCATCTAGCCGACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACAt < 1:856706/70‑1 (MQ=255) gTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACAtt < 1:3089765/71‑1 (MQ=255) ttCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGt < 1:1784880/40‑1 (MQ=255) ttCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACAt < 1:4225844/69‑1 (MQ=255) cATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACATTAAc > 1:645217/1‑71 (MQ=255) aTTGCGAAACCATCTAGCCGACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACATTa < 1:2324542/68‑1 (MQ=255) aTTGCGAAACCATCTAGCCGACAAACGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGAc < 1:831364/64‑1 (MQ=255) aTTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAg < 1:3767517/62‑1 (MQ=255) ttGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGa < 1:105127/58‑1 (MQ=255) tGCGAAACCATCTAGCCGACAAATGCTAAATTGGTTc > 1:3171323/1‑37 (MQ=255) tGCGAAACCATCTAGCCGACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACATTAACctc > 1:2639099/1‑71 (MQ=255) cGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACATTAACCtctc < 1:984558/71‑1 (MQ=255) gAAACCATCTAGCCGACAAATGCTAAATTGGTTCGCAATCAGGTATACAAATGAAAGACATTAACCTCTCa < 1:582516/71‑1 (MQ=255) aaaCCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACATTAACCTCTCAc > 1:1252841/1‑71 (MQ=255) cATCTAGCCGACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACATTAACCTCTCACCTTc < 1:301331/71‑1 (MQ=255) cATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACATTAACCTCTCa < 1:3187328/66‑1 (MQ=255) aTCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACATTaa > 1:367356/1‑58 (MQ=255) tCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGaaa < 1:45116/49‑1 (MQ=255) tAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTaca > 1:3514751/1‑38 (MQ=255) tAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGa > 1:1352253/1‑49 (MQ=255) tAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGa > 1:3617902/1‑49 (MQ=255) tAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACa > 1:3847922/1‑51 (MQ=255) aGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACATTAACCTCTCACCTTcccccc > 1:1831721/1‑71 (MQ=255) ccAACAAATGCTAAATTGGTTCGCAATCAGGTacac < 1:2714085/36‑1 (MQ=255) caacaaATGCTAAATTGGTTCGCAATCAGGTAcaca < 1:2494431/36‑1 (MQ=255) caacaaATGCTAAATTGGTTCGCAATCAGGTAcaca < 1:2950941/36‑1 (MQ=255) aacaaATGCTAAATT‑GTTCGCAATCAGGTACACAAATGAAAGACATTa < 1:2268793/48‑1 (MQ=255) | TGACCCACACGAAAGATCACATACAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACATTAACCTCTCACCTTCCACCC > W3110S.gb/1804307‑1804443 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |