| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,806,617 | T→G | 31.1% | pseudogene (1810/1900 nt) pseudogene (1326/1416 nt) |
arpB → arpB → |
ECK1718:JW5278+JW1710:b4494; hypothetical protein ECK1718:JW1710:b1721; hypothetical protein, C‑ter fragment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,806,617 | 0 | T | G | 31.1% | 55.7 / 90.0 | 87 | pseudogene (1810/1900 nt) pseudogene (1326/1416 nt) | arpB arpB | ECK1718:JW5278+JW1710:b4494; hypothetical protein ECK1718:JW1710:b1721; hypothetical protein, C‑ter fragment |
| Reads supporting (aligned to +/- strand): ref base T (31/28); new base G (21/6); total (53/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.32e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.66e-01 | |||||||||||
Only 100 of 101 total aligned reads displayed.
TATTTACACTATTGGCCGCTAAAAATCATGACAACATGTCAGCTGTTCATATAGCCATTCATCATAAGCATTATAAAACTGTAGAAACATATTATGCTGCTATTAATGCAATCAGCCAAAGCCTGAGTTTTAGTGCTGATG > W3110S.gb/1806547‑1806687 | tATTTACACTATTGGCCGCTAAAAATCATGACAACATGTCAGCTGTTCATATAGCCATTCATCATAAGCAt > 1:1080157/1‑71 (MQ=255) aTTTACACTATTGGCCGCTAAAAATCATGACAACATGTCAGCTGTTCATATAGCCATTCATCATAAGCAt > 1:4372831/1‑70 (MQ=255) aTTTACACTATTGGCCGCTAAAAATCATGACAACATGTCAGCTGTTCATATAGCCATTCATCATAAGCAt > 1:4323136/1‑70 (MQ=255) aTTTACACTATTGGCCGCTAAAAATCATGACAACATGTCAGCTGTTCATATAGCCATTCATCATAAGCAt > 1:4280392/1‑70 (MQ=255) aTTTACACTATTGGCCGCTAAAAATCATGACAACATGTCAGCTGTTCATATAGCCATTCATCATAAGCAt > 1:3843587/1‑70 (MQ=255) tttACACTATTGGCCGCTAAAAATCATGACAACATGTCAGCTGTTCATATAGCCATTCATCATAAGCATta < 1:3157066/71‑1 (MQ=255) ttACACTATTGTCCGCTAAAAATCATGACAACATGTCAGCTGTTCATATAGCCATTCATCATAAGCATta > 1:4181691/1‑70 (MQ=255) tACACTATTGGCCGCTAAAAATCATGACAACATGTCAGCTGTTCATATAGCCATTCATCATAAGCATtat > 1:3340206/1‑70 (MQ=255) acacTATTGGCCGCTAAAAATCATGACAACATGTCAGCTGTTCATATAGCCATTCATCATAAGCATtata > 1:3487642/1‑70 (MQ=255) cacTATTGGCCGCTAAAAATCATGACAACATGTCAGCTGTTCATATAGCCATTCATCATAAGCATTATaa < 1:1812995/70‑1 (MQ=255) cTATTGGCCGCTAAAAATCATGACAACATGTCAGCTGTTCATATAGCCATTCATCATAAGCATTATAAAAc < 1:2102187/71‑1 (MQ=255) tATTGGCCGCTAAAAATCATGACAACATGTCAGCTGTTCATATAGCCATTCATCATAAGCATTATAAAACt > 1:3875414/1‑71 (MQ=255) ttGGCCGCTAAAAATCATGACAACATGTCAGCTGTTCATATAGCCATTCATCATAAGCATTATaa < 1:3782852/65‑1 (MQ=255) ttGGCCGCTAAAAATCATGACAACATGTCAGCTGTTCATATAGCCATTCATCATAAGCATTATAAAACTg > 1:3902650/1‑70 (MQ=255) ttGGCAGCTAAAAATCATGACAACATGTCAGCTGTTCATATAGCCATTTATCATAAGCATTATAAAACTg > 1:4339853/1‑70 (MQ=255) tGGCCGCTAAAAATCATGACAACATGTCAGCTGTTCATATAGCCATTCATCATAAGCATTATAAAACTg > 1:3340514/1‑69 (MQ=255) cTAAAAATCATGACAACATGTCAGCTGTTCATATAGCCATTCATCATAAGCATta < 1:2360393/55‑1 (MQ=255) tAAAAATCATGACAACATGTCAGCTGTTCATATAGCCATTCATCATAAGCATTATAAAACTGTAGAAACa > 1:3211977/1‑70 (MQ=255) aaaaaTCATGACAACATGTCAGCTGTTCATATAGCCATTCATCATAAGCATTATAAAACTGTAGAAACata > 1:46312/1‑71 (MQ=255) aaaaTCATGACAACATGTCAGCTGTTCATATAGCCATTCATCATAAGCATTATAAAACTGTAGAAACatat < 1:2235432/71‑1 (MQ=255) aaaaTCATGACAACATGTCAGCTGTTCATATAGCCATTCATCATAAGCATTATAAAACTGTAGAAACatat < 1:3895330/71‑1 (MQ=255) aaaaTCATGACAACATGTCAGCTGTTCATATAGCCATTCATCATAAGCATTATAAAACTGTAGAAACatat < 1:2522400/71‑1 (MQ=255) aTCATGACAACATGTCAGCTGTTCATATAGCCATTCATCATAAGCATTATaaaa > 1:1221413/1‑54 (MQ=255) aTCATGACAACATGTCAGCTGTTCATATAGCCATTCATCATAAGCATTATaaaa > 1:539901/1‑54 (MQ=255) aTCATGACAACATGTCAGCTGTTCATATAGCCATTCATCATAAGCATTATAAAACTGTAGAAACAtatta > 1:295644/1‑70 (MQ=255) tCATGACAACATGTCAGCTGTTCATATAGCCATTCATCATAAGCATTATaa < 1:1120000/51‑1 (MQ=255) tCATGACAACATGTCAGCTGTTCATATAGCCATTCATCATAAGCATTATAAAAc < 1:4426220/54‑1 (MQ=255) tagcattacattttcatctgtactTTCATCATAAGCAGtat < 1:3825520‑M2/17‑1 (MQ=255) tagcattacattttcatctgtactTTCATCATAAGCAGtat < 1:2542498‑M2/17‑1 (MQ=255) acaTGTCAGCTGTTCATATAGCCATTCATCATAAGCATTATAAAACTGTAGAAACATATTATGCTGCTAtt < 1:2018776/71‑1 (MQ=255) acaTGTCAGCTGTTCATATAGCCATTCATCATAAGCATTATAAAACTGTAGAAACATATTATGCTGCTAtt < 1:4158064/71‑1 (MQ=255) caTGTCAGCTGTTCATATAGCCATTCATCATAAGCATtat < 1:232482/40‑1 (MQ=255) agcattacattttcatctgtactTTCATCATAAGCAGta > 1:235506‑M2/24‑39 (MQ=255) agcattacattttcatctgtactTTCATCATAAGCAGta > 1:696809‑M2/24‑39 (MQ=255) gcattacattttcatctgtactTTCATCATAAGCAGta > 1:1185985‑M2/23‑38 (MQ=255) gcattacattttcatctgtactTTCATCATAAGCAGta > 1:4359877‑M2/23‑38 (MQ=255) gcattacattttcatctgtactTTCATCATAAGCAGta > 1:747308‑M2/23‑38 (MQ=255) gcattacattttcatctgtactTTCATCATAAGCAGta > 1:290840‑M2/23‑38 (MQ=255) gcattacattttcatctgtactTTCATCATAAGCAGTATgaa > 1:3298761‑M2/23‑42 (MQ=255) cattacattttcatctgtactTTCATCATAAGCAGta < 1:2682740‑M2/16‑1 (MQ=255) cattacattttcatctgtactTTCATCATAAGCAGta < 1:1088496‑M2/16‑1 (MQ=255) gTCAGCTGTTCATATAGCCATTCATCATAAGCATTATAAAACTGTAGAAACATATTAtgc < 1:3173378/60‑1 (MQ=255) ttacattttcatctgtactTTCATCATAAGCAgtatg < 1:424725‑M2/18‑2 (MQ=255) ttacattttcatctgtactTTCATCATAAGCAgtatg > 1:4562917‑M2/20‑36 (MQ=255) ttacattttcatctgtactTTCATCATAAGCAGTATgaa > 1:2974920‑M2/20‑39 (MQ=255) tCAGCTGTTCATATAGCCATTCATCATAAGCATTATAAAACTGTAGAAACAtatta > 1:2024056/1‑56 (MQ=255) tCAGCTGTTCATATAGCCATTCATCATAAGCATTATAAAACTGTAGAAACATATTATGCTGCTATTAATg > 1:3852576/1‑70 (MQ=255) tacattttcatctgtactTTCATCATAAGCAgtatg < 1:2643648‑M2/18‑2 (MQ=255) tacattttcatctgtactTTCATCATAAGCAGtat < 1:1190993‑M2/17‑1 (MQ=255) tacattttcatctgtactTTCATCATAAGCAGtat < 1:2732529‑M2/17‑1 (MQ=255) acattttcatctgtactTTCATCATAAGCAGTATgaa > 1:2455445‑M2/18‑37 (MQ=255) acattttcatctgtactTTCATCATAAGCAGTATgaa > 1:4334062‑M2/18‑37 (MQ=255) acattttcatctgtactTTCATCATAAGCAGTATgaa > 1:683347‑M2/18‑37 (MQ=255) acattttcatctgtactTTCATCATAAGCAGTATgaa > 1:4169933‑M2/18‑37 (MQ=255) acattttcatctgtactTTCATCATAAGCAGTATgaa > 1:2400141‑M2/18‑37 (MQ=255) aGCTGTTCATATAGCCATTCATCATAAGCATTATAAAACTGTAGAAACata > 1:4415215/1‑51 (MQ=255) cattttcatctgtactTTCATCATAAGCAGTATgaa > 1:1480082‑M2/17‑36 (MQ=255) cattttcatctgtactTTCATCATAAGCAGTATgaa > 1:2858947‑M2/17‑36 (MQ=255) cattttcatctgtactTTCATCATAAGCAGTATgaa > 1:4531661‑M2/17‑36 (MQ=255) cattttcatctgtactTTCATCATAAGCAGTATgaa > 1:2607467‑M2/17‑36 (MQ=255) cattttcatctgtactTTCATCATAAGCAGTATgaa > 1:3057292‑M2/17‑36 (MQ=255) cattttcatctgtactTTCATCATAAGCAGTATgaa > 1:2443791‑M2/17‑36 (MQ=255) cattttcatctgtactTTCATCATAAGCAGTATgaa > 1:4152239‑M2/17‑36 (MQ=255) cattttcatctgtactTTCATCATAAGCAGTATgaa > 1:4599044‑M2/17‑36 (MQ=255) cTGTTCATATAGCCATTCATCATAAGCATTATAAAACTGTAGAAACATATTATGCTGCTAtt < 1:2829806/62‑1 (MQ=255) cTGTTCATATAGCCATTCATCATAAGCATTATAAAACTGTAGAAACATATTATGCTGCTATTAATGCAAt > 1:847031/1‑70 (MQ=255) ttCATATAGCCATTCATCATAAGCATTATAAAACTGTAGaaa < 1:299109/42‑1 (MQ=255) cATATAGCCATTCATCATAAGCATTATAAAACTGTAGAAACatat > 1:4302226/1‑45 (MQ=255) cATATAGCCATTCATCATAAGCATTATAAAACTGTAGAAACATATTATGCTGCTATTAATGCAATCAGcc > 1:3391369/1‑70 (MQ=255) cATATAGCCATTCATCATAAGCATTATAAAACTGTAGAAACATATTATGCTGCTATTAATGCAATCAGcc > 1:4646712/1‑70 (MQ=255) taGCCATTCATCATAAGCATTATAAAACTGTAGAAACATATTATGCTGCTATTAATGCAATCAGCCAAAg > 1:2033720/1‑70 (MQ=255) taGCCATTCATCATAAGCATTATAAAACTGTAGAAACATATTATGCTGCTATTAATGCAATCAGCCAAAGc > 1:2918397/1‑71 (MQ=255) aTTCATCATAAGCATTATAAAACTGTAGAAACATATTATGCTGCTATTAATGc < 1:3076085/53‑1 (MQ=255) ttCATCATAAGCATTATAAAACTGTAGAAACAtatta < 1:1869770/37‑1 (MQ=255) ttCATCATAAGCATTATAAAACTGTAGAAACATATTATGCTGCTATTAATGCAATCAGCCaaa < 1:4285198/63‑1 (MQ=255) ttCATCATAAGCATTATAAAACTGTAGAAACATATTATGCTGCTATTAATGCAATCAGCCAAAGCCTg < 1:4639866/68‑1 (MQ=255) catcatAAGCATTATAAAACTGTAGAAACATATTATGCTGCTAt > 1:3708640/1‑44 (MQ=255) catcatAAGCATTATAAAACTGTAGAAACATATTATGCTGCTATTAATGCAATCAGCCAAAGCCTGAGttt > 1:2997474/1‑71 (MQ=255) catcatAAGCATTATAAAACTGTAGAAACATATTATGCTGCTATTAATGCAATCAGCCAAAGCCTGAGttt > 1:2000799/1‑71 (MQ=255) catcatAAGCAATATAAAACTGTAGAAACATATTATGCTGCTATTAATGCAATCAGCCAAAGCCTGAGtt > 1:496648/1‑70 (MQ=255) atcatAAGCATTATAAAACTGTAGAAACATATTATGCTGCTATTAATGCAATCAGCCAAAGCCTGAGtttt < 1:3254130/71‑1 (MQ=255) atcatAAGCATTATAAAACTGTAGAAACATATTATGCTGCTATTAATGCAATCAGCCAAAGCCTGAGttt < 1:4490019/70‑1 (MQ=255) atcatAAGCATTATAAAACTGTAGAAACATATTATGCTGCTATTAATGCAATCAGCCAAAGCCTGAGttt < 1:2933029/70‑1 (MQ=255) atcatAAGCATTATAAAACTGTAGAAACATATTATGCTGCTATTAATGCAATCAGCCAAAGCCTGAGttt < 1:639694/70‑1 (MQ=255) atcatAAGCATTATAAAACTGTAGAAACATATTATGCTGCTATTAATGCAATCAGCCAAAGCCTGAGttt > 1:709676/1‑70 (MQ=255) tcatAAGCATTATAAAACTGTAGAAACATATTATGCTGCTATTAATGCAATCAGCCAAAGCCTGAGtttt < 1:3582020/70‑1 (MQ=255) atAAGCATTATAAAACTGTAGAAACATATTATGCTGCTa > 1:3820402/1‑39 (MQ=255) atAAGCATTATAAAACTGTAGAAACATATTATGCTGCTAtt < 1:2029904/41‑1 (MQ=255) atAAGCATTATAAAACTGTAGAAACATATTATGCTGCTATTAATGCAATCAGCCAAAGCCTGAGTTTTa > 1:868565/1‑69 (MQ=255) atAAGCATTATAAAACTGTAGAAACATATTATGCTGCTATTAATGCAATCAGCCAAAGCCTGAGTTTTAGt > 1:452848/1‑71 (MQ=255) aGCATTATAAAACTGTAGAAACATATTATGCTGCTATTAATGCAATc < 1:1692419/47‑1 (MQ=255) cATTATAAAACTGTAGAAACATATTATGCTGCTATTAATGCAATCAg > 1:1873920/1‑47 (MQ=255) cATTATAAAACTGTAGAAACATATTATGCTGCTATTAATGCAATCAg > 1:1868540/1‑47 (MQ=255) aTTATAAAACTGTAGAAACATATTATGCTGCTATTAATGCAATCAGCCAAAGCCTGAGTTTTAGTGCtgat < 1:3003238/71‑1 (MQ=255) ttATAAAACTGTAGAAACATATTATGCTGCTATTAATGCAATCAGCCAAAGCCTGAGttt < 1:2278335/60‑1 (MQ=255) ttATAAAACTGTAGAAACATATTATGCTGCTATTAATGCAATCAGCCAAAGCCTGAGttt < 1:1521070/60‑1 (MQ=255) ttATAAAACTGTAGAAACATATTATGCTGCTATTAATGCAATCAGCCAAAGCCTGAGttt < 1:1526369/60‑1 (MQ=255) ttATAAAACTGTAGAAACATATTATGCTGCTATTAATGCAATCAGCCAAAGCCTGAGTTTTAGTGCtgat > 1:3848369/1‑70 (MQ=255) ttATAAAACTGTAGAAACATATTATGCTGCTATTAATGCAATCAGCCAAAGCCTGAGATTTAGTGCtgatg < 1:4040123/71‑1 (MQ=255) ttATAAAACTGT‑‑AAACATATTATGCTGCTATTAATGCAATCAGcc < 1:1074180/45‑1 (MQ=38) | TATTTACACTATTGGCCGCTAAAAATCATGACAACATGTCAGCTGTTCATATAGCCATTCATCATAAGCATTATAAAACTGTAGAAACATATTATGCTGCTATTAATGCAATCAGCCAAAGCCTGAGTTTTAGTGCTGATG > W3110S.gb/1806547‑1806687 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |