| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,824,257 | T→C | 32.6% | F29S (TTT→TCT) | nadE → | NAD synthetase, NH3/glutamine‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,824,257 | 0 | T | C | 32.6% | 55.4 / 42.8 | 49 | F29S (TTT→TCT) | nadE | NAD synthetase, NH3/glutamine‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (17/16); new base C (5/11); total (22/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01 | |||||||||||
CAAATAATAAAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTCAGGA > W3110S.gb/1824187‑1824326 | caaATAATAAAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGAttt < 1:3069693/71‑1 (MQ=255) taaAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGaaa < 1:1899240/71‑1 (MQ=255) taaAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTCTCTGaaa < 1:2385446/71‑1 (MQ=255) aaGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTCTCTGAAAAg < 1:2941629/71‑1 (MQ=255) aGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTCTCTGAAAAGc < 1:2667409/71‑1 (MQ=255) gcgcTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGAAAAGCTa < 1:2196547/71‑1 (MQ=255) aaaaCCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGAAAAGCTACCTGCAAACtt < 1:258848/71‑1 (MQ=255) aaaCCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGAAAAGCTACCTGCAAACtt > 1:667235/1‑70 (MQ=255) aaCCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGAAAAGCTACCTGCAAACTTAt > 1:2188528/1‑71 (MQ=255) aCCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGAAAAGCTACCTGCAAACTTAt > 1:206878/1‑70 (MQ=255) aCCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTCTCTGAAAAGCTACCTGCAAACTTATc > 1:1056423/1‑71 (MQ=255) aCCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTCTCTGAAAAGCTACCTGCAAACTTATc < 1:4610065/71‑1 (MQ=255) cGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTCTCTGAAAAGCTACCTGCAAACTTATCCg > 1:577994/1‑71 (MQ=255) tAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCAtt > 1:727318/1‑70 (MQ=255) tAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTCTCTGAAAAGCTAc > 1:3329101/1‑49 (MQ=255) tGCTGAAGAGGAAATTCGTCGTAGTGTCGATTCTCTGaa < 1:2341195/39‑1 (MQ=255) tGCTGAAGAGGAAATTCGTCGTAGTGTCGATTCTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTa < 1:2122606/68‑1 (MQ=255) cTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGAAAAGCTACCTGCaaa > 1:2984871/1‑51 (MQ=255) cTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCa > 1:1361350/1‑71 (MQ=255) cTGAAGAGGAAATTCGTCGTAGTGTCGATTCTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAAt < 1:3320409/69‑1 (MQ=255) gAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGAAAAg < 1:561753/38‑1 (MQ=255) aaGAGGAAATTCGTCGTAGTGTCGATTTTCTGAAAAGCTAc > 1:694873/1‑41 (MQ=255) agGAAATTCGTCGTAGTGTCGATTCTCTGAAAAGCTACCTGCa < 1:1122691/43‑1 (MQ=255) agGAAATTCGTCGTAGTGTCGATTCTCTGAAAAGCTACCTGCa < 1:896442/43‑1 (MQ=255) gAAATTCGTCGTAGTGTCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATc < 1:274291/62‑1 (MQ=255) gAAATTCGTCGTAGTGTCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGc > 1:2611925/1‑70 (MQ=255) aaaTTCGTCGTAGTGTCGATTCTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTc > 1:2274889/1‑71 (MQ=255) ttCGTCGTAGTGTCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGtt > 1:611087/1‑49 (MQ=255) ttCGTCGTAGTGTCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGt < 1:256943/48‑1 (MQ=255) gtcgtAGTGTCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATc < 1:3328475/71‑1 (MQ=255) gtAGTGTCGATTTTCTGAAAAGCTACCTGCGAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAgc > 1:2424171/1‑71 (MQ=255) gtAGTGTCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAgc > 1:117021/1‑71 (MQ=255) gtAGTGTCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAgc > 1:4278517/1‑71 (MQ=255) gtAGTGTCGATTCTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAgc < 1:2752517/71‑1 (MQ=255) gtgtCGATTTTCTGAAAAGCTACCTGCAAACTTATc < 1:2743159/36‑1 (MQ=255) gtgtCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTa < 1:334592/45‑1 (MQ=255) tttCGATTTTCTGAAAAGCTACCTCCAAACTTATCCGTTCa < 1:869447/39‑1 (MQ=38) tgtCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCAc < 1:2530103/50‑1 (MQ=255) tgtCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGAt < 1:2979472/63‑1 (MQ=255) tgtCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAg < 1:4083911/66‑1 (MQ=255) gtCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGtt < 1:695043/38‑1 (MQ=255) tCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCa > 1:2301514/1‑39 (MQ=255) tCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAAt > 1:2449044/1‑45 (MQ=255) tCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTgg > 1:3535353/1‑51 (MQ=255) tCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGAt < 1:3712394/61‑1 (MQ=255) tCGATTCTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGt > 1:4137774/1‑71 (MQ=255) gATTTTCTGAAAATCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGa > 1:2174692/1‑58 (MQ=255) gATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCggg < 1:3693432/57‑1 (MQ=255) gATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGt > 1:1341912/1‑69 (MQ=255) gATTCTCTGAAAAGCTACCTGCAAACATATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTc < 1:4354453/70‑1 (MQ=255) ttttCTGAAAAGCTACCTGCAAACTTATCCGTTCATTaa < 1:3785552/39‑1 (MQ=255) ttttCTGAAAAGCTACCTGCAAACTTATCCGTTCATTaa < 1:3409563/39‑1 (MQ=255) ttttCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAgcggc < 1:2782074/64‑1 (MQ=255) ttttCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAgcggc < 1:2186641/64‑1 (MQ=255) tttCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCa > 1:1588235/1‑42 (MQ=255) ttCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTCAGGa > 1:2694644/1‑70 (MQ=255) | CAAATAATAAAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTCAGGA > W3110S.gb/1824187‑1824326 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |