| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,828,990 | G→A | 14.3% | H328H (CAC→CAT) | astB ← | succinylarginine dihydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,828,990 | 0 | G | A | 14.3% | 167.0 / 17.1 | 63 | H328H (CAC→CAT) | astB | succinylarginine dihydrolase |
| Reads supporting (aligned to +/- strand): ref base G (30/24); new base A (6/3); total (36/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.20e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01 | |||||||||||
AAAGACTTTTAGTTCGCTAATCGGGTTGTCAGCGGCAAGGAGTTCATTGAGATAACCCCATACTCCGGCGTGTTCCCGACACTCCTGAGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAA > W3110S.gb/1828921‑1829060 | aaaGACTTTTAGTTCGCTAATCGGGTTGTCAGCGGCAAGGAGTTCATTGAGATAACCCCATACTCCGGCg < 1:1234063/70‑1 (MQ=255) ttAGTTCGCTAATCGGGTTGTCAGCGGCAAGGAGTTCATTGAGATAACCCCATACTCCGGCGTGTTccc < 1:840653/69‑1 (MQ=255) ttAGTTCGCTAATCGGGTTGTCAGCGGCAAGGAGTTCATTGAGATAACCCCATACTCCGGCGTGTTccc < 1:4433694/69‑1 (MQ=255) tAGTTCGCTAATCGGGTTGTCAGCGGCAAGGAGTTCATTGAGATAACCCCATACTCCGGCGTGTTCCCGa < 1:2094366/70‑1 (MQ=255) aGTTCGCTAATCGGGTTGTCAGCGGCAAGGAGTTCATTGAGATAACCCCATACTCCGGCGTGTTCCCGaca > 1:969239/1‑71 (MQ=255) cGCTAATCGGGTTGTCAGCGGCAAGGAGTTCATTGAGATAACCCCATACTCCGGCATGTTCCCGACACTcc > 1:469637/1‑71 (MQ=255) cTAATCGGGTTGTCAGCGGCAAGGAGTTCATTGAGATAACCCCATACTCCGGCGTGTTCCCGACACTCCTg < 1:397551/71‑1 (MQ=255) aTCGGGTTGTCAGCGGCAAGGAGTTCATTGAGATAACCCCATACTCCGGCGTGTTCCCGACACTCCTGAgg > 1:3497125/1‑71 (MQ=255) aTCGGGTTGTCAGCGGCAAGGAGTTCATTGAGATAACCCCATACTCCGGCGTGTTCCCGACACTCCTGAg < 1:1727469/70‑1 (MQ=255) cGGGTTGTCAGCGGCAAGGAGTTCATTGAGATAACCCCATACTCCGGCGTGTTCCCGACACTCCTGAgg > 1:1851271/1‑69 (MQ=255) gggTTGTCAGCGGCAAGGAGTTCATTGAGATAACCCCATACTCCGGCATGTTCCCGACACTCCTGAGgcag > 1:3168421/1‑71 (MQ=255) gTTGTCAGCGGCAAGGAGTTCATTGAGATAACCCCATACTCCGGCATGTTCCCGACACTCCTGAGgcagca < 1:3524345/71‑1 (MQ=255) gTCAGCGGCAAGGAGTTCATTGAGATAACCCCATACTCCGGCGTGTTCCCGACACTCCTGAGgcagcagc < 1:3720481/70‑3 (MQ=255) tCAGCGGCAAGGAGTTCATTGAGATAACCCCATACTCCGGCGTGTTCCCGACACTCCt < 1:1537710/58‑1 (MQ=255) cAGCGGCAAGGAGTTCATTGAGATAACCCCATACTCCGGCGTGTTCCCGACACTCCTGAGGCAGCACCaa > 1:3082862/1‑70 (MQ=255) aGCGGCAAGGAGTTCATTGAGATAACCCCATACTCCGGCGTGTTCCCGACACTCCTGAGGCAGCACCAAca > 1:2700351/1‑71 (MQ=255) cGGCAAGGAGTTCATTGAGATAACCCCATACTCCGGCGTGTTCCCGACACTCCTGAGGCAGCACCAAcat > 1:3684028/1‑70 (MQ=255) ggCAAGGAGTTCATTGAGATAACCCCATACTCCGGCGTGTTCCCGACACTCCTGAGgcagc < 1:1403466/61‑1 (MQ=255) gCAAGGAGTTCATTGAGATAACCCCATACTCCGGCGTGTTCCCGACACt < 1:2920335/49‑1 (MQ=255) ttCATTGAGATAACCCCATACTCCGGCGTGTTccc < 1:295805/35‑1 (MQ=255) ttCATTGAGATAACCCCATACTCCGGCGTGTTCCCGACACTCCTGAGgcagca > 1:4631719/1‑53 (MQ=255) ttCATTGAGATAACCCCATACTCCGGCGTGTTCCCGACACTCCTGAGGCAGCAc > 1:1969704/1‑54 (MQ=255) ttCATTGAGATAACCCCATACTCCAGCGTGTTCCCGACACTCCTGAGGCAGCAc > 1:4494207/1‑54 (MQ=255) cATTGAGATAACCCCATACTCCGGCGTGTTCCCGacac > 1:2952133/1‑38 (MQ=255) cATTGAGATAACCCCATACTCCGGCGTGTTCCCGACACTCCTGAGGCAGCACCAACATCATGGAACcatca > 1:2820/1‑71 (MQ=255) cATTGAGATAACCCCATACTCCGGCATGTTCCCGACACTCCTGAGGCAGCACCAAc > 1:1892388/1‑56 (MQ=255) cATTGAGATAACCCCATACTCCGGCATGTTCCCGACACTCCTGAGGCAGCACCAACATCATGGAACcatc > 1:1188505/1‑70 (MQ=255) aTTGAGATAACCCCATACTCCGGCATTTTCCCGACACTCCTGAGGCAGCACCAACATCATGGAACcatca < 1:3871718/70‑1 (MQ=255) ttGAGATAACCCCATACTCCGGCGTGTTCCCGACACTCCTGa < 1:3202744/42‑1 (MQ=255) tcagaTAACCCCATACTCCGGCGTGTTCCCGACACTCCTGAGGCAGCACCAACATCATGGAACCATCATcg > 1:3643298/3‑71 (MQ=255) tGAGATAACCCCATACTCCGGCGTGTTCCCGACACTCCTGAgg > 1:2855720/1‑43 (MQ=255) tGAGATAACCCCATACTCCGGCATGTTCCCGACACTCCTGAGGCAGCACCAACATCATGGAACCATCATcg > 1:4606293/1‑71 (MQ=255) gagaTAACCCCATACTCCGGCGTGTTCCCGACACTCCTGAGgcagca > 1:28985/1‑47 (MQ=255) gagaTAACCCCATACTCCGGCGTGTTCCCGACACTCCTGAGgcagca > 1:1797596/1‑47 (MQ=255) gagaTAACCCCATACTCCGGCGTGTTCCCGACACTCCTGAGgc > 1:250763/1‑43 (MQ=255) gagaTAACCCCATACTCCGGCGTGTTCCCGACACTCCTGAGGCAGCACCAACATCATGGa > 1:3581321/1‑60 (MQ=255) gagaTAACCCCATACTCCGGCGTGTTCCCGACACTCCTGAGGCAGCACCAACATCATGGa > 1:3284876/1‑60 (MQ=255) aaCACCATACTCCGGCGTGTTCCCGACACTCCTGAGGCAGCACCAACATCATGGAACcatca > 1:4258937/1‑62 (MQ=255) aCCCCATACTCCGGGGTGTTCCCGACACTCCTGAGGCAGCACCAACATCATGGAACCATCATCGCGGCt > 1:2065632/1‑69 (MQ=255) aCCCCATACTCCGGCGTGTTCCCGACACTCCTGAGGCAGCACCAACATCATGGAACCATCATCGCGGCt > 1:786832/1‑69 (MQ=255) aCCCCATACTCCGGCGTGTTCCCGACACTCCTGAGGCAGCACCAACATCATGGAACCATCATCGCGGCt > 1:1301830/1‑69 (MQ=255) cccATACTCCGGCGTGTTCCCGACACTCCTGAGGCAGCACCAACATCATGGAACCATCATCGCGGCTcagc < 1:1218664/71‑1 (MQ=255) ccATACTCCGGCGTGTTCCCGACACTCCTGAGGCAGCACCAACATCATGGAACCATCATCGCGGCTcagca < 1:2095633/71‑1 (MQ=255) cATACTCCGGCGTGTTCCCGACACTCCTGAGGCAGCACCAACATCATGGAACCATCATCGCGGCTcagcag > 1:3209823/1‑71 (MQ=255) cATACTCCGGCGTGTTCCCGACACTCCTGAGGCAGCACCAACATCATGGAACCATCATCGCGGCTcagcag > 1:2327301/1‑71 (MQ=255) cATACTCCGGCGTGTTCCCGACACTCCTGAGGCAGCACCAACATCATGGAACCATCATCGCGGCTcagcag > 1:2569908/1‑71 (MQ=255) tACTCCGGCGTGTTCCCGACACTCCTGAGGCAGCAcc < 1:327167/37‑1 (MQ=255) tACTCCGGCGTGTTCCCGACACTCCTGAGGCAGCAcc < 1:3126728/37‑1 (MQ=255) tACTCCGGCGTGTTCCCGACACTCCTGAGGCAGCACCAAcatcat < 1:2481033/45‑1 (MQ=255) tACTCCGGCGTGTTCCCGACACTCCTGAGGCAGCACCAACATCATGGAACcatc < 1:3340025/54‑1 (MQ=255) tACTCCGGCGTGTTCCCGACACTCCTGAGGCAGCACCAACATCATGGAACcat < 1:2818843/53‑1 (MQ=255) tACTCCGGCGTGTTCCCGACACTCCTGAGGCAGCACCAACATCATGGAACca < 1:1527076/52‑1 (MQ=255) cTCCGGCGTGTTCCCGACACTCCTGAGGCAGCACCaa > 1:3298354/1‑37 (MQ=255) cTCCGGCGTGTTCCCGACACTCCTGAGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTgg > 1:1534355/1‑71 (MQ=255) cTCCGGCGTGTTCCCGACACTCCTGAGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTgg > 1:4099445/1‑71 (MQ=255) cTCCGGCATGTTCCCGACACTCCTGAGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTg < 1:3183156/70‑1 (MQ=255) tCCGTCATGTTCCCGACACTCCTGAGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGc > 1:730389/1‑71 (MQ=255) cGGCGTGTTCCCGACACTCCTGAGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGc < 1:2672445/69‑1 (MQ=255) cGGCGTGTTCCCGACACTCCTGAGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGc < 1:3980129/69‑1 (MQ=255) cGGCGTGTTCCCGACACTCCTGAGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCt > 1:556252/1‑70 (MQ=255) ggCGTGTTCCCGACACTCCTGAGGCAGCACCATCATCATGGAACCATCATCGCGGCTCAGCAGTTGGc < 1:2478899/68‑1 (MQ=255) ggCGGGTTCCCGACACTCCTGAGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGc < 1:875396/68‑1 (MQ=255) cGTGTTCCCGACACTCCTGAGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTg > 1:1154728/1‑64 (MQ=255) cGTGTTCCCGACACTCCTGAGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTa < 1:4077354/71‑1 (MQ=255) gtgtTCCCGACACTCCTGAGGCAGCACCAACATCATgg > 1:1920728/1‑38 (MQ=255) gtgtTCCCGACACTCCTGAGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTaa < 1:2058674/71‑1 (MQ=255) | AAAGACTTTTAGTTCGCTAATCGGGTTGTCAGCGGCAAGGAGTTCATTGAGATAACCCCATACTCCGGCGTGTTCCCGACACTCCTGAGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAA > W3110S.gb/1828921‑1829060 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |