Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	1,885,888	A→G	17.0%	T329T (ACA→ACG)	yeaV →	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,885,888	0	A	G	17.0%	107.1 / 16.4	47	T329T (ACA→ACG)	yeaV	predicted transporter
Reads supporting (aligned to +/- strand):  ref base A (16/23);  new base G (3/5);  total (19/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.04e-01

AGCTATGCCATTCATCAGTTTATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTAAATTGTTCCTCGCCGCA  >  W3110S.gb/1885829‑1885957                                                            |                                                                      aGCTATGCCATTCATCAGTTTATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAACACTGGgcggcg                                                             >  1:1133792/1‑70 (MQ=255) aGCTATGCCATTCATCAGTTTATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGa                                                            <  1:2520066/71‑1 (MQ=255)   cTATGCCATTCATCAGTTTATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGaaa                                                          <  1:2125521/71‑1 (MQ=255)      tGCCATTCATCAGTTTATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAAc                                                         <  1:1293341/69‑1 (MQ=255)       gCCATTCATCAGTTTATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAg                                                       >  1:1908444/1‑70 (MQ=255)        ccATTCATCAGTTTATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAaca                                                                       >  1:4369490/1‑53 (MQ=255)        ccATTCATCAGTTTATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAAc                                                         >  1:3420606/1‑67 (MQ=255)         cATTCATCAGTTTATCAATGGCGTAATCAACGTCCCACTGGTGCTGGAAACACTGGGCGGCGAAACAGc                                                      <  1:666544/69‑1 (MQ=255)         cATTCATCAGTTTATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTg                                                    <  1:1073099/71‑1 (MQ=255)          aTTCATCAGTTTATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTg                                                    >  1:677558/1‑70 (MQ=255)           ttCATCAGTTTATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAAc                                                         <  1:1191675/64‑1 (MQ=255)           ttCATCAGTTTATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTa                                                  <  1:7885/71‑1 (MQ=255)                 aGTTTATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGc                                              >  1:1991342/1‑69 (MQ=255)                     tATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAacac                                                                      >  1:4451072/1‑41 (MQ=255)                     tATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTc                                        >  1:1350246/1‑71 (MQ=255)                      aTCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAaca                                                                       <  1:270161/39‑1 (MQ=255)                      aTCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAaca                                                                       >  1:1185322/1‑39 (MQ=255)                       tCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCt                                       <  1:590449/70‑1 (MQ=255)                       tCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCt                                       <  1:470098/70‑1 (MQ=255)                       tCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCt                                       <  1:4310041/70‑1 (MQ=255)                       tCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTg                                      <  1:4338185/71‑1 (MQ=255)                         aaTGGCGTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGaa                                                           <  1:3173189/48‑1 (MQ=255)                           tGGCGTAATCAACGTCCCACAGGTGCTGGAAACGCTGGGCGGCGAAACAGCTGTACAGCAAg                                           <  1:3851381/62‑1 (MQ=255)                              cGTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGt                               <  1:2275656/71‑1 (MQ=255)                              cGTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGt                               <  1:4430814/71‑1 (MQ=255)                               gTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGtt                              >  1:1789507/1‑71 (MQ=255)                                tAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGtt                              <  1:2745705/70‑1 (MQ=255)                                  aTCAACGTCCCACAGGTGCTGGAAACGCTGGGCGGCGa                                                            <  1:340633/38‑1 (MQ=255)                                  aTCAACGTCCCACAGGTGCTGGAAACGCTGGGCGGCGAAACAGCTGTa                                                  <  1:1529994/48‑1 (MQ=255)                                  aTCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGa                                     >  1:4241235/1‑61 (MQ=255)                                  aTCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGt                                  >  1:4250612/1‑64 (MQ=255)                                  aTCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGc                            >  1:4267012/1‑70 (MQ=255)                                   tCAATGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGc                            >  1:29493/1‑69 (MQ=255)                                   tCAACGTCCCACAGGTTCTGGAAACACTGGGCGGCGAAACAg                                                       <  1:2440650/42‑1 (MQ=255)                                    cAACGTCCCACAGGTGCTGGAAACGCTGGGCGGCGaaa                                                          >  1:3577016/1‑38 (MQ=255)                                          cccACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGt                                                   <  1:4477596/39‑1 (MQ=255)                                          cccACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGt                    >  1:1137565/1‑70 (MQ=255)                                          accACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGt                    >  1:4143585/2‑70 (MQ=255)                                             acaGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTAAAt                >  1:3920783/1‑71 (MQ=255)                                              caGGTGCTGGAAACGATGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTAAAtt               >  1:1742898/1‑71 (MQ=255)                                              caGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTa                                                  >  1:3110132/1‑36 (MQ=255)                                               aGGTGCTGGAAACGCTGGGCGGCGAAACAGCTGTACAGCAAGTTc                                        >  1:507218/1‑45 (MQ=255)                                               aGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTAAATTg              <  1:1749577/71‑1 (MQ=255)                                                 gTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTaaa                 <  1:2667130/66‑1 (MQ=255)                                                  tGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTAAATTGTTc           <  1:2954864/71‑1 (MQ=255)                                                  tGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTAAATTGTTc           <  1:1949815/71‑1 (MQ=255)                                                    cTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTAAATTGTTCCt         <  1:4357092/71‑1 (MQ=255)                                                      ggAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTAAAtt               >  1:1428712/1‑63 (MQ=255)                                                       gAAACGCTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTAAATTGTTCCTcg       <  1:929368/70‑1 (MQ=255)                                                       gAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTAAATTGTTCCTcgc      <  1:3482144/71‑1 (MQ=255)                                                         aaCGCTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTAAATTGTTCCTcgccg    <  1:3116801/71‑1 (MQ=255)                                                           cacTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGa                                     >  1:4362886/1‑36 (MQ=255)                                                           cacTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGa                                     >  1:2734167/1‑36 (MQ=255)                                                            acTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTAAATTGTTCCTCGCCGCa  >  1:1803766/1‑70 (MQ=255)                                                            |                                                                      AGCTATGCCATTCATCAGTTTATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTAAATTGTTCCTCGCCGCA  >  W3110S.gb/1885829‑1885957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑