Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	1,912,571	T→C	10.9%	N194N (AAT→AAC)	yebQ →	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,912,571	0	T	C	10.9%	169.0 / 12.1	64	N194N (AAT→AAC)	yebQ	predicted transporter
Reads supporting (aligned to +/- strand):  ref base T (34/23);  new base C (2/5);  total (36/28)
Fisher's exact test for biased strand distribution p-value = 2.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

ATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTGCCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGC  >  W3110S.gb/1912505‑1912641                                                                   |                                                                       aTTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTGCCACCCAATGGt                                                                     >  1:527214/1‑70 (MQ=255)       aaTCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTGCCACCCAATGGTTCTcgc                                                               <  1:2308775/70‑1 (MQ=255)          cAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTGCCACCCAATGGTTCTCgcgc                                                             >  1:3729241/1‑69 (MQ=255)             cGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTGCCACCCAATGGTtct                                                                  <  1:21278/61‑1 (MQ=255)             cGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTGCCACCCAATGGTTCTCGCGCCAGt                                                         >  1:86614/1‑70 (MQ=255)             cGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTGCCACCCAACGGTTCTCGCGCCAGTa                                                        <  1:4047234/71‑1 (MQ=255)             cGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTGCCACCCAACGGTTCTCGCGCCAGTa                                                        <  1:1837618/71‑1 (MQ=255)                 ccGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTGCCACCCAATGGTTCTCGCGCCAGTAAAcc                                                    >  1:1046376/1‑71 (MQ=255)                      aGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTGCCACCCAATGGTTCTCGCGCCAGTAAACCCCGtt                                               >  1:3818830/1‑71 (MQ=255)                      aGGTATTATCGCCCTGCTTCTGGAGATGCGTTTTCTGCCACCCAATGGTTCTCGCGCCAGTAAACCCCGtt                                               >  1:2180812/1‑71 (MQ=255)                        gTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTGCCACCCAAt                                                                        <  1:2943110/44‑1 (MQ=255)                         tattatCGCCCTGCTTCTGGCGATGCGTTTTCTGCCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCg                                            <  1:839783/71‑1 (MQ=255)                         tattatCGCCCTGCTTCTGGCGATGCGTTTTCTGCCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCg                                            <  1:4254427/71‑1 (MQ=255)                         tattatCGCCCTGCTTCTGGCGATGCGTTTTCTGCCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCg                                            <  1:2872582/71‑1 (MQ=255)                          attatCGCCCTGCTTCTGGCGATGCGTTTTCTGCCACCCAATGGtt                                                                    >  1:2786566/1‑46 (MQ=255)                          attatCGCCCTGCTTCTGGCGATGCGTTTTCTGCCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGa                                           >  1:3305186/1‑71 (MQ=255)                          attatAGCCCTGCTTCTGGCGATGCGTTTTCTGCCACCCAATGGTTCTCGCGCCAGTAAAc                                                     >  1:3366468/1‑61 (MQ=255)                           ttatCGCCCTGCTTCTGGCGATGCGTTTTCTGCCACCCAATGGTTCTCtcgc                                                             <  1:1981437/52‑1 (MQ=255)                           ttatCGCCCTGCTTCTGGCGATGCGTTTTCTGCCACCCAATGGTTCTCGCGCCAGTAAACCCCGt                                                <  1:2824851/65‑1 (MQ=255)                           ttatCGCCCTGCTTCTGGCGATGCGTTTTCTGCCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGAc                                          >  1:3573100/1‑71 (MQ=255)                            tatCGCGCTGCTTCTGGCGATGCGTTTTCTGCCACCCAATGGTTCTCGCGCCAg                                                          <  1:3641751/54‑1 (MQ=255)                            tatCGCCCTGCTTCTGGCGATGCGTTTTCTGCCACCCAATGGTTCTCGCGCCAGTAAAcccc                                                  <  1:1346430/62‑1 (MQ=255)                             atCGCCCTGCTTCTGGCGATGCGTTTTCTGCCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCt                                        >  1:4037740/1‑71 (MQ=255)                             atCGCCCTGCTTCTGGCGATGCGTTTTCTGCCACCCAACGGTTCTCGCGCCAGTAAACCCCGTTTCGACCt                                        >  1:1018511/1‑71 (MQ=255)                              tCGCCCTGCTTCTGGCGATGCGTTTTCTGCCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTc                                             >  1:1541108/1‑65 (MQ=255)                              tCGCCCTGCTTCTGGCGATGCGTTTTCTGCCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGAc                                          >  1:1648501/1‑68 (MQ=255)                              tCGCCCTGCTTCTGGCGATGCGTTTTCTGCCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTg                                       >  1:3436236/1‑71 (MQ=255)                                 cccTGCTTCTGGCGATGCGTTTTCTGCCACCCAAt                                                                        <  1:3809179/35‑1 (MQ=255)                                  ccTGCTTCTGGCGATGCGTTTTCTGCCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCa                                   <  1:3238273/71‑1 (MQ=255)                                    tGCTTCTGGCGATGCGTTTTCTGCCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCg                                            <  1:2085360/60‑1 (MQ=255)                                    tGCTTCTGGCGATGCGTTTTCTGCCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCg                                            <  1:1412710/60‑1 (MQ=255)                                     gCTTCTGGCGATGCGTTTTCTGCCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGAc                                          >  1:1460785/1‑61 (MQ=255)                                     gCTTCTGGCGATGCGTTTTCTGCCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGAc                                          >  1:3959480/1‑61 (MQ=255)                                      cTTCTGGCGATGCGTTTTCTGCCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGAc                                          >  1:4272415/1‑60 (MQ=255)                                      cTTCTGGCGATGCGTTTTCTGCCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAgcgc                               >  1:4522870/1‑71 (MQ=255)                                      cTTCTGGCGATGCGTTTTCTGCCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAgcgc                               >  1:2838873/1‑71 (MQ=255)                                      cTTCTGGCGATGCGTTTTCTGCCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAgcgc                               >  1:2831047/1‑71 (MQ=255)                                             cGATGCGTTTTCTGCCACCCAACGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGtgatg                        <  1:3472408/71‑1 (MQ=255)                                               aTGCGTTTTCTGCCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGaa                      >  1:4637202/1‑71 (MQ=255)                                               aTGCGTTTTCTGCCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGaa                      <  1:749727/71‑1 (MQ=255)                                                tGCGTTTTCTGCCACCCAACGGTTCTCGCGCCAGTAAACCCCGt                                                <  1:138377/44‑1 (MQ=255)                                                tGCGTTTTCTGCCACCCAACGGTTCTCGCGCCAGTAAACCCCGt                                                <  1:673630/44‑1 (MQ=255)                                                 gCGTTTTCTGCCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAAcg                    >  1:1540973/1‑71 (MQ=255)                                                 gCGTTTTCTGCCACCCAATCGTTCTCCCGCCAGTAAAccc                                                   >  1:1743636/1‑40 (MQ=38)                                                 gCGTTTTCTGCCACCCAACGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAAcg                    >  1:1720910/1‑71 (MQ=255)                                                  cGTTTTCTGCCACCCAATGGTTCTCGCGCCAGTAAAc                                                     >  1:3119014/1‑37 (MQ=255)                                                  cGTTTTCTGCCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCg                                            <  1:4536442/46‑1 (MQ=255)                                                  cGTTTTCTGCCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAAcgc                   <  1:2590257/71‑1 (MQ=255)                                                    ttttCTGCCACCCAATGGTTCTCGCGCCAGTAAACCCCGt                                                <  1:2623496/40‑1 (MQ=255)                                                    ttttCTGCCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGtga                          <  1:158727/62‑1 (MQ=255)                                                      ttCTGCCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGtgatg                        >  1:4211372/1‑62 (MQ=255)                                                      ttCTGCCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGtgat                         >  1:2636704/1‑61 (MQ=255)                                                      ttCTGCCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGt                 >  1:1301080/1‑69 (MQ=255)                                                      ttCTGCCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGt                 >  1:2215021/1‑69 (MQ=255)                                                        cTGCCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGt                            >  1:530957/1‑56 (MQ=255)                                                           ccACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAg                                  >  1:2596471/1‑47 (MQ=255)                                                           ccACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGtga                          <  1:3647537/55‑1 (MQ=255)                                                           ccACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAAc             <  1:4137871/68‑1 (MQ=255)                                                             aCCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCg        <  1:2949632/71‑1 (MQ=255)                                                                cAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGtgatg                        <  1:2382180/52‑1 (MQ=255)                                                                cAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGt                            <  1:3285056/48‑1 (MQ=255)                                                                cAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGt                 >  1:3554704/1‑59 (MQ=255)                                                                cAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGcc     >  1:479732/1‑71 (MQ=255)                                                                 aaTGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGaa                      >  1:4207738/1‑53 (MQ=255)                                                                 aaTGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCt    >  1:2687628/1‑71 (MQ=255)                                                                  aTGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTg   <  1:129742/71‑1 (MQ=255)                                                                   tGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCAGCCTGc  <  1:598073/71‑1 (MQ=255)                                                                   |                                                                       ATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTGCCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGC  >  W3110S.gb/1912505‑1912641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑