| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,940,955 | T→C | 10.5% | D318G (GAT→GGT) | lpxM ← | myristoyl‑acyl carrier protein (ACP)‑dependent acyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,940,955 | 0 | T | C | 10.5% | 97.0 / 4.0 | 38 | D318G (GAT→GGT) | lpxM | myristoyl‑acyl carrier protein (ACP)‑dependent acyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (17/17); new base C (2/2); total (19/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTTGAACTTATCATCAGGCGAAGGCCTCTCCTCGCGAGAGGCTTTTTTATTTGATGGGATAAAGATCTTTGCGCTTATACGGCTGGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTATCCAGGTGTA > W3110S.gb/1940890‑1941022 | cTTGAACTTATCATCAGGCGAAGGCCTCTCCTCGCGAGAGGCTTTTTTATTTGATGGGATAAAGATCTTTg < 1:2975723/71‑1 (MQ=255) tGAACTTATCATCAGGCGAAGGCCTCTCCTCGCGAGAGGCTTTTTTATTTGATGGGATAAAGATCTTTgc > 1:1772525/1‑70 (MQ=255) gAACTTATCATCAGGCGAAGGCCTCTCCTCGCGAGAGGCTTTTTTATTTGATGGGATAAAGATCTTTgcgc > 1:4367610/1‑71 (MQ=255) aaCTTATCATCAGGCGAAGGCCTCTCCTCGCGAGAGGCTTTTTTATTTGATGGGATAAAGATCTTTGCGCt < 1:2342423/71‑1 (MQ=255) cTTATCATCAGGCGAAGGCCTCTCCTCGCGAGAGGCTTTTTTATTTGATGGGATAAAGATCTTTGCGCTta < 1:4350450/71‑1 (MQ=255) cTTATCATCAGGCGAAGGCCTCTCCTCGCGAGAGGCTTTTTTATTTGATGGGATAAAGATCTTTGCGCTta < 1:2416615/71‑1 (MQ=255) catcaGGCGAAGGCCTCTCCTCGCGAGAGGCTTTTTTATTTGATGGGATAAAGATCTTTGCGCTTATACgg > 1:3738599/1‑71 (MQ=255) ggCGAAGGCCTCTCCTCGCGAGAGGCTTTTTTATTTGATGGGATAAAGATCTTTGCGCTTATACGGCTGGa < 1:3568309/71‑1 (MQ=255) ctctCCTCGCGAGAGGCTTTTTTATTTGATGGGATAAAGATCTTTGCGCTTATACGGCTGGATTTCGCCCg > 1:2600575/1‑71 (MQ=255) ctctCCTCGCGAGAGGCTTTTTTATTTGATGGGATAAAGACCTTTGCGCTta > 1:2627393/1‑52 (MQ=255) tctcCTCGCGAGAGGCTTTTTTATTTGATGGGATAAAGATCTTTGCGCTTATACGGCTGGATTTCGcc < 1:2719635/68‑1 (MQ=255) tcctcGCGAGAGGCTTTTTTATTTGATGGGATAAAGATCt < 1:1216016/40‑1 (MQ=255) tcctcGCGAGAGGCTTTTTTATTTGATGGGATAAAGATCt > 1:1348827/1‑40 (MQ=255) tcctcGCGAGAGGCTTTTTTATTTGATGGGATAAAGATCTTTGCGCTtata > 1:4115050/1‑51 (MQ=255) tcctcGCGAGAGGCTTTTTTATTTGATGGGATAAAGATCTTTGCGCTTATACGGCTGGATTTCGCCCGGta > 1:1644980/1‑70 (MQ=255) ctcGCGAGAGGCTTTTTTATTTGATGGGATAAAGATCTTTGCGCTTATACGGCTGGATTTCGCCCGGTTTg > 1:2645904/1‑71 (MQ=255) ctcGCGAGAGGCTTTTTTATTTGATGGGATAAAGACCTTTGCGCTTATACGGCTGGAt < 1:909087/58‑1 (MQ=255) ctcGCGAGAGGCTTTTTTATTTGATGGGATAAAGACCTTTGCGCTTATACGGCTGGAt < 1:2770012/58‑1 (MQ=255) gcgAGAGGCTTTTTTATTTGATGGGATAAAGATCTTTGCGCt > 1:1043868/1‑42 (MQ=255) gcgAGAGGCTTTTTTATTTGATGGGATAAAGATCTTTGCGCTTATACGGCTGGa > 1:4007530/1‑54 (MQ=255) gcgAGAGGCTTTTTTATTTGATGGGATAAAGATCTTTGCGCTTATACGGCTGGATTTCGCCCGGTTTGc > 1:1362073/1‑69 (MQ=255) agagGCTTTTTTATTTGATGGGATAAAGATCTTTGCGCTTATACGGCTGGATTTCGCCCGGTTTGCGAGtt < 1:784187/71‑1 (MQ=255) gagGCTTTTTTATTTGATGGGATAAAGACCTTTGCGCTTATACGGCTGGATTTCGCCCGGTTTGCGAGttt > 1:2021491/1‑71 (MQ=255) agGCTTTTTTATTTGATGGGATAAAGATCTTTGCGCTTATACGGCTg < 1:1391524/47‑1 (MQ=255) ttttttATTTGATGGGATAAAGATCTTTGCGCTTATACGGCTGGATTTCGCCCGGTTTGCGAGTTTTCAg > 1:3806414/1‑70 (MQ=255) tttttATTTGATGGGATAAAGATCTTTGCGCTTATACGGCTGGATTTCGCCCGGTTTGCGAGtt < 1:1871973/64‑1 (MQ=255) ttttATTTGATGGGATAAAGATCTTTGCGCTTATACGGCTGGATTTCGCCCGGTTTGCGAGtt < 1:99859/63‑1 (MQ=255) ttttATTTGATGGGATAAAGATCTTTGCGCTTATACGGCTGGATTTCGCCCGGTTTGCGAGTTTTCAGCaa > 1:2222801/1‑71 (MQ=255) aTTTGATGGGATAAAGATCTTTGCGCTTATACGGCTGGATTTCGCCCGGTTTGCGAGTTTTCAGCAAttt < 1:3176011/70‑1 (MQ=255) aTTTGATGGGATAAAGATCTTTGCGCTTATACGGCTGGATTTCGCCCGGTTTGCGAGTTTTCAGCAAttt < 1:731009/70‑1 (MQ=255) gATGGGATAAAGATCTTTGCGCTTATACGGCTGGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTa > 1:2301253/1‑71 (MQ=255) gATGGGATAAAGATCTTTGCGCTTATACGGCTGGATTTCGCCCGGTTTGCGAGTATTCAGCAATTTTAGt > 1:3702145/1‑70 (MQ=255) gggATAAAGATCTTTGCGCTTATACGGCTGGATTTCGCCCGGTTTGc < 1:1947336/47‑1 (MQ=255) ggATAAAGATCTTTGCGCTTATAAGGCTGGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTATct < 1:3072711/70‑2 (MQ=255) aTAAAGATCTTTGCGCTTATACGGCTGGATTTCGCCCTGTTTGCGAg < 1:4340374/47‑1 (MQ=255) aaGATCTTTGCGCTTATACGGCTGGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTATCCAg > 1:2942390/1‑67 (MQ=255) aGATCTTTGCGCTTATACGGCTGGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTATcc < 1:3353166/64‑1 (MQ=255) aGATCTTTGCGCTTATACGGCTGGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTATCCAGGTGTa > 1:2333613/1‑71 (MQ=255) tCTTTGCGCTTATACGGCTGGATTTCGCCCGGTTTGCGAGTTTTCAGCAAt < 1:1320936/51‑1 (MQ=255) | CTTGAACTTATCATCAGGCGAAGGCCTCTCCTCGCGAGAGGCTTTTTTATTTGATGGGATAAAGATCTTTGCGCTTATACGGCTGGATTTCGCCCGGTTTGCGAGTTTTCAGCAATTTTAGTATCCAGGTGTA > W3110S.gb/1940890‑1941022 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |