| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,002,183 | G→A | 23.2% | R114C (CGC→TGC) | fliY ← | cystine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,002,183 | 0 | G | A | 23.2% | 136.4 / 38.4 | 69 | R114C (CGC→TGC) | fliY | cystine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (31/22); new base A (4/12); total (35/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.40e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01 | |||||||||||
TTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCG > W3110S.gb/2002113‑2002253 | ttACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTgcg < 1:3396252/71‑1 (MQ=255)ttACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTgcg < 1:3467670/71‑1 (MQ=255) ttttttCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCtcat > 1:3626154/1‑71 (MQ=255) ttttCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCtcat > 1:2864428/1‑69 (MQ=255) aCCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAAt < 1:2432414/71‑1 (MQ=255) cAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATg < 1:4110347/70‑1 (MQ=255) gcgcCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGGACTCATCAGAAATGGTg < 1:4001539/71‑1 (MQ=255) gAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACtcatca < 1:3512615/56‑1 (MQ=255) aaTACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAg > 1:2954882/1‑56 (MQ=255) aaTACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAg > 1:4448265/1‑56 (MQ=255) aaTACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTg < 1:1174550/69‑1 (MQ=255) aaTACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGa < 1:2164311/70‑1 (MQ=255) aaTACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGAt > 1:2526816/1‑71 (MQ=255) aaTACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGAt > 1:3380281/1‑71 (MQ=255) tACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCtc < 1:370483/49‑1 (MQ=255) tACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGa < 1:2755816/55‑1 (MQ=255) tACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCCTGCACTCATCAGAAATGGTGACCTGATTa < 1:4176039/71‑1 (MQ=255) ccAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAAt > 1:3199610/1‑71 (MQ=255) gAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATcac < 1:2352277/71‑1 (MQ=255) aTGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTgcgc > 1:3001241/1‑39 (MQ=255) aTGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGaa > 1:468476/1‑48 (MQ=255) gtgtACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACAt > 1:1007536/1‑70 (MQ=255) tgtACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATgg < 1:1586860/48‑1 (MQ=255) tACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTaa < 1:1151908/58‑1 (MQ=255) cGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGaaa > 1:2355973/1‑41 (MQ=255) cGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGaaa > 1:3490846/1‑41 (MQ=255) cGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATAc > 1:3584503/1‑71 (MQ=255) cGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATAc > 1:835062/1‑71 (MQ=255) cGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATAc > 1:4374142/1‑71 (MQ=255) ggggTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACg > 1:564727/1‑71 (MQ=255) ggggTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATc < 1:2439650/57‑1 (MQ=255) gggTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGt > 1:4558513/1‑71 (MQ=255) gggTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGt > 1:1969363/1‑71 (MQ=255) gggTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGt > 1:602488/1‑71 (MQ=255) ggTTGAGAAATCGTATTTTTTCTTGCGCTCATCAg < 1:3689923/35‑1 (MQ=255) ggTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGtt < 1:2534199/71‑1 (MQ=255) ttGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAAt < 1:3550145/37‑1 (MQ=255) ttGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACAt < 1:2485184/60‑1 (MQ=255) ttGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTa < 1:56531/71‑1 (MQ=255) gagaAATCGTATTTTTTCTTGCGCTCATCAGAAATg < 1:3375475/36‑1 (MQ=255) gagaAATCGTATTTTTTCTTGCACTCATCAGAAATGGTg < 1:455004/39‑1 (MQ=255) gagaAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGa > 1:1008641/1‑40 (MQ=255) gagaAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGCCCTGATTAATCACCACATCAATACGTTTa > 1:306307/1‑69 (MQ=255) agaAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGa > 1:3234007/1‑44 (MQ=255) agaAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGa > 1:111125/1‑44 (MQ=255) agaAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGa > 1:4610477/1‑44 (MQ=255) gaAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGt < 1:1947994/71‑1 (MQ=255) aaaTCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGa > 1:2561947/1‑42 (MQ=255) aaaTCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGa > 1:77275/1‑42 (MQ=255) aaaTCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGa > 1:3624841/1‑42 (MQ=255) aaTCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATcac < 1:2980413/49‑1 (MQ=255) aaTCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTcc < 1:1149611/71‑1 (MQ=255) cGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGc > 1:1053098/1‑71 (MQ=255) gTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCg < 1:1689728/71‑1 (MQ=255) gTATTTTTTCTTGCACTCATAAGAAATGGTGACCt > 1:3166288/1‑35 (MQ=38) aTTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATca > 1:1145790/1‑42 (MQ=255) aTTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAAGCACCACATCAATACGTTTAGAGTCCAGCGa < 1:3461749/70‑1 (MQ=255) ttttttCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTaga < 1:2264163/60‑1 (MQ=255) ttttttCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTaga < 1:3464908/60‑1 (MQ=255) ttttttCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTa < 1:2875347/58‑1 (MQ=255) ttttttCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTa < 1:1511923/58‑1 (MQ=255) tttttCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTa < 1:3178343/57‑1 (MQ=255) tttttCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGa > 1:1876128/1‑68 (MQ=255) tttttCTTGCACTCATCAGAAATGGTGACCTGATTa < 1:4594413/36‑1 (MQ=255) tttttCTTGCACTCATCAGAAATGGTGACCTGATTa < 1:3663034/36‑1 (MQ=255) ttttCTTGCGCTCATCAGAAATGGTGACCTGATTAAt > 1:3183302/1‑37 (MQ=255) ttttCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATa > 1:2704306/1‑50 (MQ=255) ttttCTTGCGCTCATCAGAAATGGTGACATGATTAAt > 1:2746967/1‑37 (MQ=255) tttCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGt > 1:115372/1‑52 (MQ=255) tCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCaa > 1:1359007/1‑45 (MQ=255) tCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAg < 1:2992521/70‑1 (MQ=255) ttGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTa < 1:678528/51‑1 (MQ=255) gcTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCg < 1:3055620/71‑1 (MQ=255) gcTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCg < 1:1903177/71‑1 (MQ=255) gcTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCg < 1:3060946/71‑1 (MQ=255) | TTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCG > W3110S.gb/2002113‑2002253 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |