| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,081,459 | A→G | 11.3% | F36L (TTT→CTT) | yeeX ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,081,459 | 0 | A | G | 11.3% | 158.3 / 11.3 | 62 | F36L (TTT→CTT) | yeeX | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (30/25); new base G (5/2); total (35/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACGGATCTTTTTCTCAACGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCTGATAATGTGAGAGTCGGA > W3110S.gb/2081391‑2081528 | aCGGATCTTTTTCTCAACGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAAtt > 1:13219/1‑71 (MQ=255)aCGGATCTTTTTCTCAACGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAAtt > 1:1853887/1‑71 (MQ=255) cGGATCTTTTTCTCAACGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGTAACAGACGAACAAATTc < 1:4318169/71‑1 (MQ=255) cTTTTTCTCAACGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAg < 1:2176455/69‑1 (MQ=255) cTTTTTCTCAACGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAg < 1:1950247/69‑1 (MQ=255) ttttCTCAACGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACaaa > 1:1679300/1‑61 (MQ=255) tttCTCAACGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAg < 1:2232590/66‑1 (MQ=255) tttCTCAACGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTAc > 1:4350382/1‑69 (MQ=255) tttCTCAACGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACg < 1:147766/70‑1 (MQ=255) tttCTCAACGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGt > 1:390152/1‑71 (MQ=255) tcAACGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTg > 1:142904/1‑71 (MQ=255) cAACGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTg > 1:4150698/1‑70 (MQ=255) cAACGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTg > 1:1982101/1‑70 (MQ=255) aaCGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTgg > 1:934855/1‑70 (MQ=255) cGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAAt > 1:165319/1‑71 (MQ=255) gTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATg < 1:1373173/71‑1 (MQ=255) cTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACaaa > 1:42441/1‑48 (MQ=255) cTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAg < 1:3357291/71‑1 (MQ=255) cTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAg > 1:2036616/1‑71 (MQ=255) gAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGc > 1:658055/1‑71 (MQ=255) aaTTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCt < 1:4210844/71‑1 (MQ=255) tttCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTg > 1:564161/1‑71 (MQ=255) tCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTgg > 1:3850589/1‑70 (MQ=255) tCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGGACGta > 1:2954259/1‑52 (MQ=255) cACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTgg > 1:1535544/1‑69 (MQ=255) ttGCAGTTTGTTCTTACGACGGAACAGACGAACAAGTTc < 1:543967/39‑1 (MQ=255) ttGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGttt > 1:2166186/1‑71 (MQ=255) ttGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGt > 1:605504/1‑69 (MQ=255) gCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGttt > 1:4006227/1‑69 (MQ=255) aGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAt < 1:2919404/71‑1 (MQ=255) tttGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTc < 1:2816193/66‑1 (MQ=255) ttGTTCTTACGACGGAACAGACGAACAAATTCCAGTACg < 1:1815245/39‑1 (MQ=255) ttGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTgg < 1:2929756/45‑1 (MQ=255) tGTTCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt > 1:340681/1‑70 (MQ=255) tGTTCTTACGACGGAACAGACGAACAAATTCCAGt > 1:1349396/1‑35 (MQ=255) tGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTc > 1:2941202/1‑40 (MQ=255) tGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTgg < 1:4263271/44‑1 (MQ=255) tGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt > 1:255094/1‑70 (MQ=255) tGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAt < 1:2997034/67‑1 (MQ=255) ttCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTta < 1:450249/70‑1 (MQ=255) ttCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTta < 1:1313511/70‑1 (MQ=255) ttCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTta < 1:538529/70‑1 (MQ=255) ttCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTta < 1:1225426/70‑1 (MQ=255) ttCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTta < 1:150565/70‑1 (MQ=255) tCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTtata > 1:1514020/1‑71 (MQ=255) acgacgGAACAGACGAACAAATTCCAGTACGTCCTGGaa > 1:1952214/1‑39 (MQ=255) acgacgGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTtata > 1:1634544/1‑67 (MQ=255) acgacgGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATAc < 1:150544/68‑1 (MQ=255) cgacgGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCt < 1:3863977/71‑1 (MQ=255) gacgGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTta > 1:722058/1‑63 (MQ=255) gacgGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTta > 1:186978/1‑63 (MQ=255) ggAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTgg > 1:2790327/1‑45 (MQ=255) ggAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCTGATa < 1:1462871/71‑1 (MQ=255) aCAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCTGATAAtg > 1:3398354/1‑71 (MQ=255) aCAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGtt < 1:1649810/47‑1 (MQ=255) aGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAttt < 1:3424870/52‑1 (MQ=255) gACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTta > 1:4058374/1‑54 (MQ=255) aCGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCTGATAAtgt > 1:2324245/1‑68 (MQ=255) gAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTta > 1:3946859/1‑51 (MQ=255) gAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTtata > 1:962259/1‑53 (MQ=255) gAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCTGATAATGTgag < 1:668690/69‑1 (MQ=255) aaCAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt < 1:3220614/48‑1 (MQ=255) aCAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCTGATAATGTGagag > 1:1349815/1‑69 (MQ=255) cAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCTGATAAt > 1:1474679/1‑61 (MQ=255) aaTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCTGATAATGTGAGAGTCGGa < 1:2122229/71‑1 (MQ=255) aTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAttt > 1:1386635/1‑42 (MQ=255) | ACGGATCTTTTTCTCAACGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCTGATAATGTGAGAGTCGGA > W3110S.gb/2081391‑2081528 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |