Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 2,117,348 C→T 44.4% G229D (GGC→GAC)  wcaM ← predicted colanic acid biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,117,3480CT44.4% 15.0 / 56.3 45G229D (GGC→GAC) wcaMpredicted colanic acid biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base C (17/8);  new base T (12/8);  total (29/16)
Fisher's exact test for biased strand distribution p-value = 7.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.64e-01

ATTCGCCACGACAAAGTTTTTCACTGCCTGGTCTTCCGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGCCTATGCCGATGCCCCAGTTGATTTTGCCGTTGGTACAGTTGATGCGCTCGATGACATGGTCA  >  W3110S.gb/2117278‑2117410
                                                                      |                                                              
atTCGCCACGACAAAGTTTTTCACTGCCTGGTCTTCCGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGc                                                                >  1:2613981/1‑71 (MQ=255)
   cGCCACGACAAAGTTTTTCACTGCCTGGTCTTCCGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGCCTa                                                             >  1:2454386/1‑71 (MQ=255)
       aCGACAAAGTTTTTCACTGCCTGGTCTTCCGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGCCTATGcc                                                         >  1:14842/1‑71 (MQ=255)
        cGACAAAGTTTTTCACTGCCTGGTCTTCCGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGTCTATGCCg                                                        <  1:96200/71‑1 (MQ=255)
          aCAAAGTTTTTCACTGCCTGGTCTTCCGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGCCTATGCCGAt                                                      >  1:1399446/1‑71 (MQ=255)
          aCAAAGTTTTTCACTGCCTGGTCTTCCGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGCCTATGCCGAt                                                      >  1:2707833/1‑71 (MQ=255)
          aCAAAGTTTTTCAATGCCTGGTCTTCCGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGTCTATGCCGAt                                                      >  1:55282/1‑71 (MQ=255)
           cAAAGTTTTTCACTGCCTGGTCTTCCGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGTCTATGCCGa                                                       >  1:1594977/1‑69 (MQ=255)
              aGTTTTTCACTGCCTGGTCTTCCGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGCCTATGCCGATGccc                                                  >  1:2001821/1‑71 (MQ=255)
              aGTTTTTCACTGCCTGGTCTTCCGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGCCTATGCCGATGcc                                                   >  1:1883495/1‑70 (MQ=255)
               gTTTTTCACTGCCTGGTCTTCCGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGTCTATGCCGATGccc                                                  >  1:1337215/1‑70 (MQ=255)
               gTTTTTCACTGCCTGGTCTTCCGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGTCTATGCCGATGccc                                                  >  1:2680280/1‑70 (MQ=255)
                    tCACTGCCTGGTCTTCCGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGCCTATGCCGATGCCCCAGtt                                             >  1:4340045/1‑70 (MQ=255)
                    tCACTGCCTGGTCTTCCGGGTAGTTGTTATCATAAGTGCTTACCGCAAGGCCTATGCCGATGCCCCAGtt                                             >  1:2452752/1‑70 (MQ=255)
                      aCTGCCTGGTCTTCCGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGcc                                                               <  1:3752268/50‑1 (MQ=255)
                         gCCTGGTCTTCCGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGTCTATGCCGATGCCCCAGTTGAtttt                                       >  1:875342/1‑71 (MQ=255)
                          ccTGGTCTTCCGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGTCTATGCCGATGCCCCAGTTGAttt                                        <  1:3343674/69‑1 (MQ=255)
                             ggTCTTCCGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGTCTATGCCGa                                                       >  1:3804912/1‑51 (MQ=255)
                              gTCTTCGGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGCCTATGCCGATGCCCTAGTTGTTTTTGcc                                    <  1:2225594/69‑1 (MQ=255)
                              gTCTTCCGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGCCTATGCCGATGCCCCAGTTGATTTTGCCg                                   <  1:785270/70‑1 (MQ=255)
                              gTCTTCCGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGCCTATGCCGATGCCCCAGTTGATTTTGCCGt                                  >  1:602501/1‑71 (MQ=255)
                                 ttCCGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGCCTATGCCGATGCCCCAGTTGa                                           <  1:2001702/59‑1 (MQ=255)
                                    cGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGTCTATGCCGATGCCCCAGTTGATTTTGCCGTTGGTAc                            <  1:258842/71‑1 (MQ=255)
                                     gggTAGTTGTTATCATAAGTGCTTCCCGCAAGGCCTATGcc                                                         <  1:199586/41‑1 (MQ=255)
                                      ggTAGTTGTTATCATAAGTGCTTCCCGCAAGGTCTATGCCGATGCCCCAGTTGATTTTGCCGTTGGTACAg                          <  1:4242502/71‑1 (MQ=255)
                                        tAGTTGTTATCATAAGTGCTTCCCGCAAGGTCTATGCCGATGCCCCAGTTGATTTTGCCGTTGGTACAGtt                        <  1:2368332/71‑1 (MQ=255)
                                        tAGTTGTTATCATAAGTGCTTCCCGCAAGGCCTATGCCGATGCCCCAGTTGATTTTGCCGTTGGTACAGtt                        <  1:4191782/71‑1 (MQ=255)
                                          gttgttATCATAAGTGCTTCCCGCAAGGTCTATGCCGATGCCCCAGTTGATTTTGCCGTTg                                >  1:106466/1‑61 (MQ=255)
                                           ttgttATCATAAGTGCTTCCCGCAAGGTCTATGCCGATGCCCCAGTTGATTTTGCCGt                                  <  1:3741861/58‑1 (MQ=255)
                                            tgttATCATAAGTGCTTCCCGCAAGGTCTATGCCGATGCCCCAGTTGATTTTGCCGTTGGTACAGtt                        <  1:2676999/67‑1 (MQ=255)
                                             gttATCATAAGTGCTTCCCGCAAGGTCTATGCCGATGCCCCAGTTGATTTTGCCg                                   >  1:3641369/1‑55 (MQ=255)
                                             gttATCATAAGTGCTTCCCGCAAGGTCTATGCCGATGCCCCAGTTGATTTTGCCGTTg                                >  1:1371066/1‑58 (MQ=255)
                                             gttATCATAAGTGCTTCCCGCAAGGTCTATGCCGATGCCCCAGTTGATTTTGCCGTTGGTACAGTTGATgc                   >  1:544424/1‑71 (MQ=255)
                                             gttATCATAAGTGCTTCCCGCAAGGCCTATGCCGATGCCCCAg                                               >  1:2368398/1‑43 (MQ=255)
                                             gttATCATAAGTGCTTCCCGCAAGGCCTATGCCGATGCCCCAGTTGATTTTGCCGTTGGTACAGTTGATgc                   >  1:2430333/1‑71 (MQ=255)
                                                 tCATAAGTGCTTCCCGCAAGGCCTATGCCGATGCCCCAGTTGATTTTGCCGTTGGTACAGTTGATGCGCTc               >  1:3449005/1‑71 (MQ=255)
                                                    tAAGTGCTTCCCGCAAGGTCTATGCCGATGCCCCAGTTGATTTTGCCGTTGGTACAGTTGATGCGCTCGAt            <  1:2153025/71‑1 (MQ=255)
                                                      aGTGCTTCCCGCAAGGTCTATGCCGATGCCCCAGTTGATTTTGCCGTTGGTACAGTTGATGCGCTCGATGa          >  1:1573175/1‑71 (MQ=255)
                                                      aGTGCTTCCCGCAAGGCCTATGCCGATGCCCCAGTTGATTTTGCCGTTGGTACAGTTGATGCGCTCGATGa          >  1:2534716/1‑71 (MQ=255)
                                                      aGTGCTTCCCGCAAGGCCTATGCCGATGCCCCAGTTGATTTTGCCGTTGGTACAGTTGATGCGCTCGATGa          <  1:4395378/71‑1 (MQ=255)
                                                             cccGCAAGGCCTATGCCGATGCCCCAGTTGATTTTGCCGTTGGTa                             >  1:1659675/1‑45 (MQ=255)
                                                             cccGCAAGGCCTATGCCGATGCCCCAGTTGATTTTGCCGTTGGTa                             >  1:1491648/1‑45 (MQ=255)
                                                             cccGCAAGGCCTATGCCGATGCCCCAGTTGATTTTGCCGTTGGTACAGTTGATGCGCTCGATGACATGGt    >  1:1796219/1‑70 (MQ=255)
                                                              ccGCAAGGCCTATGCCGATGCCCCAGTTGATTTTGCCGTTGGTACAGTTGATGCGCTCGATGACATGGTc   <  1:1574208/70‑1 (MQ=255)
                                                              ccGCAAGGCCTATGCCGATGCCCCAGTTGATTTTGCCGTTGGTACAGTTGATGCGCTCGATGACATGGTCa  <  1:456257/71‑1 (MQ=255)
                                                               cGCAAGGCCTATGCCGATGCCCCAGTTGATTTTGCCGTTGGTAc                            >  1:2415499/1‑44 (MQ=255)
                                                                 cAAGGTCTATGCCGATGCCCCAGTTGATTTTGCCGTTGGTACAGTTGATGCGCTCGATGACATg      >  1:701296/1‑64 (MQ=255)
                                                                      ccTATGCCGATGCCCCAGTTGATTTTGCCGTTGGTACAGTTGATGCGCTCGATGACa        >  1:2111517/1‑57 (MQ=255)
                                                                      |                                                              
ATTCGCCACGACAAAGTTTTTCACTGCCTGGTCTTCCGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGCCTATGCCGATGCCCCAGTTGATTTTGCCGTTGGTACAGTTGATGCGCTCGATGACATGGTCA  >  W3110S.gb/2117278‑2117410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: