| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,141,886 | A→G | 7.8% | intergenic (+264/+10) | yegH → / ← asmA | fused predicted membrane proteins/predicted assembly protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,141,886 | 0 | A | G | 7.8% | 131.8 / 3.9 | 51 | intergenic (+264/+10) | yegH/asmA | fused predicted membrane proteins/predicted assembly protein |
| Reads supporting (aligned to +/- strand): ref base A (28/19); new base G (2/2); total (30/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.63e-01 | |||||||||||
ATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGC > W3110S.gb/2141822‑2141953 | atgatgCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCGCCTgg < 1:895592/70‑1 (MQ=255)atgatgCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGt < 1:2184519/71‑1 (MQ=255)atgatgCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGGCACCTGGt < 1:1352019/71‑1 (MQ=255) aCGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACAtct > 1:3979057/1‑71 (MQ=255) gAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACAtcttc < 1:3473157/71‑1 (MQ=255) gAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACAtcttc > 1:2049052/1‑71 (MQ=255) aaCGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCAcc > 1:499611/1‑53 (MQ=255) gATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTa < 1:1088279/51‑1 (MQ=255) gATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACAtc < 1:1418013/60‑1 (MQ=255) aTGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACa > 1:789958/1‑57 (MQ=255) tGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAAct > 1:3825824/1‑71 (MQ=255) tGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAAct > 1:4647730/1‑71 (MQ=255) aCGCTTTACGCGTCTTATCAGGCCTACAGGACTGTTCACCTGGTAACCTACATCTTCTCCAGCAActtctt < 1:1430745/71‑1 (MQ=255) aCGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCGcc < 1:3750870/40‑1 (MQ=255) aCGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAActtctt < 1:263374/71‑1 (MQ=255) aCGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAAct < 1:371273/67‑1 (MQ=255) aCGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAAct < 1:1715349/67‑1 (MQ=255) tttACGCGTCTTATCAGGCCTACAGGACTGGTCAcc < 1:921404/36‑1 (MQ=255) aCGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCaa > 1:1435347/1‑58 (MQ=255) cgTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTg < 1:3571097/71‑1 (MQ=255) gTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTcac < 1:1219086/63‑1 (MQ=255) tCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAActtctt > 1:2774683/1‑59 (MQ=255) tCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGc > 1:4476337/1‑70 (MQ=255) cTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACAtctt > 1:2347709/1‑43 (MQ=255) cTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACAtctt > 1:4246312/1‑43 (MQ=255) cTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCGACTTCTTCACAt > 1:4409166/1‑63 (MQ=255) ttATCAGGCCTACAGGACTGGTCGCCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCAt > 1:2286517/1‑71 (MQ=255) ttATCAGGCCTACAGGACTGGTCACCTGGTAACCTACa > 1:3917975/1‑38 (MQ=255) ttATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCAt > 1:1152815/1‑71 (MQ=255) aTCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTa > 1:1929748/1‑71 (MQ=255) aGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTa > 1:2622733/1‑68 (MQ=255) aGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACgg > 1:3709071/1‑71 (MQ=255) gCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTa > 1:2470992/1‑66 (MQ=255) tACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCAt < 1:3417647/61‑1 (MQ=255) tACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGa < 1:455161/68‑1 (MQ=255) tACAGGACTGGACGCCTGGTAACCTACATCTTCTCCAGCAACTTCTTcac > 1:4627116/1‑50 (MQ=39) aCAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATc < 1:3846821/70‑1 (MQ=255) cAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAActtctt > 1:3642676/1‑45 (MQ=255) cAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAActtctt > 1:892980/1‑45 (MQ=255) cAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAActt > 1:3639657/1‑42 (MQ=255) cAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCtt > 1:965830/1‑71 (MQ=255) cAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCtt > 1:4635117/1‑71 (MQ=255) aGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAActt > 1:3271499/1‑41 (MQ=255) aGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCa > 1:2762017/1‑57 (MQ=255) ggACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACttcttc < 1:2947905/44‑1 (MQ=255) ggACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACttcttc < 1:2204866/44‑1 (MQ=255) gACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCtt < 1:3608287/68‑1 (MQ=255) gACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTAt > 1:2819146/1‑71 (MQ=255) cTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTc < 1:1555211/71‑1 (MQ=255) gTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCtt > 1:3777843/1‑63 (MQ=255) gTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGc > 1:4638605/1‑71 (MQ=255) aCCTGGTAACCTACATCTTCTCCAGCAACTTCTTAACATCTTTGCCATTACGGGAATCTTTATTc > 1:2831722/1‑65 (MQ=255) | ATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGC > W3110S.gb/2141822‑2141953 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |