| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,178,242 | G→A | 13.6% | P48S (CCT→TCT) | gatA ← | galactitol‑specific enzyme IIA component of PTS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,178,242 | 0 | G | A | 13.6% | 114.9 / 9.6 | 44 | P48S (CCT→TCT) | gatA | galactitol‑specific enzyme IIA component of PTS |
| Reads supporting (aligned to +/- strand): ref base G (19/19); new base A (2/4); total (21/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.66e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01 | |||||||||||
TCCTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGTGGCCAGGTATCATGAACCACACCTTTGGCGAGCATC > W3110S.gb/2178175‑2178309 | gacttagCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGaa > 1:3442424‑M2/8‑71 (MQ=255)gacttagCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGaa < 1:3224510‑M2/64‑1 (MQ=255) ttagCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTc > 1:3296542‑M2/5‑71 (MQ=255) agCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTc < 1:1546101‑M2/67‑1 (MQ=255) aTGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGAGAATTCTGCtt < 1:3527343/71‑1 (MQ=255) tGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGc > 1:883851/1‑68 (MQ=255) cTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCa < 1:2796721/70‑1 (MQ=255) tCACAATGCGGTATTGCAATAGCGTGCTTCTCAAGCATTATCCCGGTAGGGAATTCTGCTtct > 1:2920102/1‑63 (MQ=255) tCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCaa > 1:2323923/1‑70 (MQ=255) tCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGAGAATTCTGc < 1:898464/59‑1 (MQ=255) acaATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGAGAATTCTGCtt > 1:134606/1‑59 (MQ=255) aTGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATg > 1:421899/1‑71 (MQ=255) tGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGaa < 1:1397986/47‑1 (MQ=255) cGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGcc < 1:2038443/70‑1 (MQ=255) tATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGAGAATTCTGCTtct < 1:3724299/52‑1 (MQ=255) ttGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCtt < 1:234397/48‑1 (MQ=255) ttGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCtt < 1:3712992/48‑1 (MQ=255) ttGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGcc < 1:2544071/65‑1 (MQ=255) ttGCAATAGCGTGCTGCTAAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGTGGc > 1:3892524/1‑71 (MQ=255) gCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGTGGc > 1:2919021/1‑69 (MQ=255) gCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGTGGc > 1:3550280/1‑69 (MQ=255) gCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGTGGc > 1:1562789/1‑69 (MQ=255) gCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGTGGc > 1:317876/1‑69 (MQ=255) gCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGACTGTGGc > 1:3707822/1‑69 (MQ=255) aaTAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGTGGCCAgg < 1:4370165/71‑1 (MQ=255) aTAGCGTGCTGCTCAAGCATTATCCCGGTAGGaaa < 1:2063739/35‑1 (MQ=255) aTAGCGTGCTGCTCAAGCATTATCCCGGTAGGGaa < 1:579840/35‑1 (MQ=255) aTAGCGTGCTGCTCAAGCATTATCCCGGTAGGGaa < 1:4621229/35‑1 (MQ=255) aTAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGc < 1:1917283/41‑1 (MQ=255) tAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAAtt > 1:3719508/1‑36 (MQ=255) gCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTg > 1:880760/1‑45 (MQ=255) gCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTg > 1:441830/1‑45 (MQ=255) gCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGTGGCCAGGTAt < 1:231295/70‑1 (MQ=255) gCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGTGGCCAGGTAt < 1:1123685/70‑1 (MQ=255) gCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGTGGCCAGGTAt > 1:3950882/1‑70 (MQ=255) gCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGTGGCCAGGTAt < 1:3139665/70‑1 (MQ=255) gCGTGCTGCTCAAGCATTATCCCGGTAGAGAATTCTGCTTCTCTGGCa > 1:4595038/1‑48 (MQ=255) tgctgcTCAAGCACTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGTGGCCAGGTATCATg < 1:4164827/71‑1 (MQ=255) gctgctCAAGCATTATCCCGGTAGAGAATTCTGCTTCTCTGGCAATTAATGCCTGTGGCCAgg < 1:4379462/63‑1 (MQ=255) ctgctCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGTGGCCAGGTATCATGaa < 1:401057/71‑1 (MQ=255) cATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTaa > 1:3045373/1‑39 (MQ=255) tATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGTGGCCAGGTATc > 1:2510119/1‑54 (MQ=255) aTCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGTGGCCAGGTATCATGAACCACACCTTTGGc > 1:1688626/1‑71 (MQ=255) tAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGTGGCCAGGTATCATGAACCACACCTTTGGCGAGCAtc < 1:2789739/71‑1 (MQ=255) | TCCTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGTGGCCAGGTATCATGAACCACACCTTTGGCGAGCATC > W3110S.gb/2178175‑2178309 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |