Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	2,183,814	T→A	9.3%	V129E (GTG→GAG)	yegU →	predicted hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,183,814	0	T	A	9.3%	109.5 / 4.7	43	V129E (GTG→GAG)	yegU	predicted hydrolase
Reads supporting (aligned to +/- strand):  ref base T (19/20);  new base A (2/2);  total (21/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

CCGACCTCGAAGATTGCGCTTAACGCCATTCGCGACGGTAAACCCGTTGCTGAACTGGAAAACAACGGCGTGACCAACGGCGCAGCGATGCGCGTCTCGCCATTAGGTTGTTTGCTTCCGGCGCGTGATGTTGA  >  W3110S.gb/2183744‑2183877                                                                       |                                                                ccGACCTCGAAGATTGCGCTTAACGCCATTCGCGACGGTAAACCCGTTGCTGAACTGGAAAACAACGGCGt                                                                 >  1:4102783/1‑71 (MQ=255) ccGACCTCGAAGATTGCGCTTAACGCCATTCGCGACGGTAAACCCGTTGCTGAACTGGAAAACAACGGCGt                                                                 >  1:2353115/1‑71 (MQ=255) ccGACCTCGAAGATTGCGCTTAACGCCATTCGCGACGGTAAAACCGTTGCTGAACTGGAAAACAACGGCGt                                                                 >  1:4160278/1‑71 (MQ=255)  cGACCTCGAAGATTGCGCTTAACGCCATTCGCGACGGTAAACCCGTTGCTGAACTGGAAAACAACGGCGt                                                                 <  1:2437576/70‑1 (MQ=255)    aCCTCGAAGATTGCGCTTAACGCCATTCGCGACGGTAAACCCGTTGCTGAACTGGAAAACAACGGCGTGa                                                               >  1:3908299/1‑70 (MQ=255)      cTCGAAGATTGCGCTTAACGCCATTCGCGACGGTAAACCCGTTGCTGAACTGGAAAACAACGGCGTGAcc                                                             >  1:4545165/1‑70 (MQ=255)      cTCGAAGATTGCGCTTAACGCCATTCGCGACGGTAAACCCGTTGCTGAACTGGAAAACAACGGCGTGAcc                                                             >  1:3006381/1‑70 (MQ=255)      cTCGAAGATTGCGCTTAACGCCATTCGCGACGGTAAACCCGTTGCTGAACTGGAAAACAACGGCGTGAcc                                                             >  1:3930163/1‑70 (MQ=255)         gAAGATTGCGCTTAACGCCATTCGCGACGGTAAACCCGTTGCTGAACTGGAAAACAACGGCGTGACCAACg                                                         >  1:4196464/1‑71 (MQ=255)         gAAGATTGCGCTTAACGCCATTCGCGACGGTAAACCCGTTGCTGAACTGGAAAACAACGGCGTGACCAACg                                                         >  1:404727/1‑71 (MQ=255)         gAAGATTGCGCTTAACGCCATTCGCGACGGTAAACCCGTTGCTGAACTGGAAAACAACGGCGAGACCaa                                                           <  1:3512489/69‑1 (MQ=255)         gAAGATTGCGCTTAACGCCATTCGCGACCGTAAACCCGTTGCTGAACTGGAAAACAACGGCGTGACCAACg                                                         >  1:1730227/1‑71 (MQ=255)           aGATTGCGCTTAACCCCATTCGCGACGGTAAACCCGTTGCTGAACTGGAAAACAACGGCGTGACCAACg                                                         <  1:290353/69‑1 (MQ=255)                 cgcTTAACGCCATTCGCGACGGTAAACCCGTTGCTGAACTGGAAAACAACGGCGTGACCAACGGCGCAGc                                                  <  1:4367357/70‑1 (MQ=255)                  gcTTAACGCCATTCGCGACGGTAAACCCGTTGCTGAACTGGAAAACAACGGCGAGACCAACGGCGCAGc                                                  <  1:3103119/69‑1 (MQ=255)                       aCGCCATTCGCGACGGTAAACCCGTTGCTGAACTGGAAAACAACGGCGTGACCAACGGCGCAGCGa                                                >  1:3624863/1‑66 (MQ=255)                        cGCCATTCGCGACGGTAAACCCGTTGCTGAACTGGAAAACAACGGCGTGAcc                                                             <  1:2114396/52‑1 (MQ=255)                        cGCCATTCGCGACGGTAAACCCGTTGCTGAACTGGAAAACAACGGCGTGACCAACGGCGCAGc                                                  <  1:368003/63‑1 (MQ=255)                           cATTCGCGACGGTAAACCCGTTGCTGAACTGGAAAACAACGGCGTGACCAACGGCGCAGc                                                  <  1:3142534/60‑1 (MQ=255)                           cATTCGCGACGGTAAACCCGTTGCTGAACTGGAAAACAACGGCGTGACCAACGGCGCAGc                                                  <  1:848944/60‑1 (MQ=255)                           cATTCGCGACGGTAAACCCGTTGCTGAACTGGAAAACAACGGCGTGACCAACGGCGCAGCGATGCGCGtc                                        >  1:3151139/1‑70 (MQ=255)                            aTTCGCGACGGTAAACCCGTTGCTGAACTGGAAAACAACGGCGTGACCAACGGCGCAGCGa                                                >  1:2554714/1‑61 (MQ=255)                               cgcgACGGTAAACCCGTTGCTGAACTGGAAAACAACGGCGTGACCAACGGCGCAGCGATGCGCGTCTCGc                                    <  1:2952741/70‑1 (MQ=255)                               cgcgACGGTAAACCCGTTGCTGAACTGGAAAACAACGGCGTGACCAACGGCGCAGCGATGCGCGTCTCGc                                    >  1:1010975/1‑70 (MQ=255)                               cgcgACGGTAAACCCGTTGCTGAACTGGAAAACAACGGCGTGACCAACGGCGCAGCGATGCGCGTCTCGc                                    <  1:1036110/70‑1 (MQ=255)                                gcgACGGTAAACCCGTTGCTGAACTGGAAAACAACGGCGTGACCAACGGCGCAGCTATGCGCGTCTCGcc                                   <  1:3087963/70‑1 (MQ=255)                                       tAAACCCGTTGCTGAACTGGAAAACAACGGCGTGACCAACGGCGCAGCGATGCGCGTCTCGCCATTAg                              <  1:4254987/68‑1 (MQ=255)                                        aaaCCCGTTGCTGAACTGGAAAACAACGGCGTGACCaa                                                           >  1:2045078/1‑38 (MQ=255)                                         aaCCCGTTGCTGAACTGGAAAACAACGGCGTGACCAACGGCGCAGCGATGCGCGTCTCGCCATTAGgttgt                         >  1:2879647/1‑71 (MQ=255)                                         aaCCCGTTGCTGAACTGGAAAACAACGGCGTGACCAACGGCGCAGCGATGCGCGTCTCGCCATTAGgttgt                         >  1:3958839/1‑71 (MQ=255)                                         aaCCCGTTGCTGAACTGGAAAACAACGGCGTGACCAACGGCGCAGCGATGCGCGTCTCGCCATTAGgttgt                         >  1:1853498/1‑71 (MQ=255)                                         aaCCAGTTGCTGAACTGGAAAACAACGGCGTGACCAACGGCGCAGCGATGCGCGtct                                       >  1:1994235/1‑57 (MQ=255)                                             cGTTGCTGAACTGGAAAACAACGGCGAGACCAACGGCGCAGCGa                                                >  1:3426394/1‑44 (MQ=255)                                             cGTTGCTGAACTGGAAAACAACGGCGAGACCAACGGCGCAGCGa                                                >  1:509453/1‑44 (MQ=255)                                              gTTGCTGAACTGGAAAACAACGGCGTGACCAACGGCGCAGCGATg                                              >  1:328958/1‑45 (MQ=255)                                               ttGCTGAACTGGAAAACAACGGCGTGACCAACGGCGCAGCGATGCGCGTCTCGc                                    <  1:2374866/54‑1 (MQ=255)                                                tGCTGAACTGGAAAACAACGGCGTGACCAACGGCGCAGc                                                  <  1:2253282/39‑1 (MQ=255)                                                 gCTGAACTGGAAAACAACGGCGTGACCAACGGCGCAGCGATGCGCGTCTCGCCATTAGGTTGTTTGCTTcc                 >  1:4411434/1‑71 (MQ=255)                                                 gCTGAACTGGAAAACAACGGCGTGACCAACGGCGCAGCGATGCGCGTCTCGCCATTAGGTTGTTTGCTTc                  <  1:1483444/70‑1 (MQ=255)                                                    gAACTGGAAAACAACGGCGTGACCAACGGCGCAGc                                                  <  1:1731509/35‑1 (MQ=255)                                                      aCTGGAAAACAACGGCGTGACCAACGGCGCAGCGATGCGCGTCTCGCCATTAGGTTGTTTGCTTCCGgcgc            >  1:276889/1‑71 (MQ=255)                                                       cTGGAAAACAACGGCGTGACCAACGGCGCAGCGATGCGCGTCTCGCCATTAGGTTGttt                       <  1:2174888/59‑1 (MQ=255)                                                          gAAATCAACGGCGTGACCAACGGCGCAGCGATGCGCGTCTCGCCATTAGGTTGTTTGCTTCCGGCGCGt          <  1:3729138/69‑1 (MQ=255)                                                              acaacGGCGTGACCAACGGCGCAGCGATGCGCGTCTCGCCATTAGGTTGTTTGCTTCCGGCGCGTGATGtt    <  1:1443818/71‑1 (MQ=255)                                                              acaacGGCGTGACCAACGGCGCAGCGATGCGCGTCTCGCCATTAGGTTGTTTGCTTCCGGCGCGTGATGtt    <  1:632735/71‑1 (MQ=255)                                                                aacGGCGTGACCAACGGCGCAGCGATGCGCGTCTCGCCATTAGGTTGTTTGCTTCCGGCGCGTGATGTTGa  <  1:1538855/71‑1 (MQ=255)                                                                      gTGACCAACGGCGCAGCGATGCGCGTCTCGCCATTAGGTTGTTTGCTTc                  <  1:1112662/49‑1 (MQ=255)                                                                       tGACCAACGGCGCAGCGATGCGCGTCTCGCCATTAGGTTGttt                       <  1:657411/43‑1 (MQ=255)                                                                       |                                                                CCGACCTCGAAGATTGCGCTTAACGCCATTCGCGACGGTAAACCCGTTGCTGAACTGGAAAACAACGGCGTGACCAACGGCGCAGCGATGCGCGTCTCGCCATTAGGTTGTTTGCTTCCGGCGCGTGATGTTGA  >  W3110S.gb/2183744‑2183877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑