| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,196,545 | G→A | 13.6% | R320C (CGT→TGT) | mrp ← | antiporter inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,196,545 | 0 | G | A | 13.6% | 175.9 / 17.2 | 66 | R320C (CGT→TGT) | mrp | antiporter inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (30/27); new base A (6/3); total (36/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01 | |||||||||||
CGGTAGATAGCGGTAAATTCGCTCTCCGGACGGCTGATAACCGTTGGCGTACCTTTATCGAGATCCTCACGTAAGGAGATGTGTAACGGCATCTGCCCCAGCAGCTGTGTATGGTATTTTTCAGCCAGTTTCTCTG > W3110S.gb/2196475‑2196610 | cGGTAGATAGCGGTAAATTCGCTCTCCGGACGGCTGATAACCGTTGGCGTACCTTTATCGAGATCCTCACg < 1:3934055/71‑1 (MQ=255) gTAGATAGCGGTAAATTCGCTCTCCGGACGGCTGATAACCGTTGGCGTACCTTTATCGAGATCCTCACGt > 1:1108969/1‑70 (MQ=255) gATAGCGGTAAATTCGCTCTCCGGACGGCTGATAACCGTTGGCGTACCTTTATTGAGATCCTCACGTAAg > 1:3805102/1‑70 (MQ=255) aTAGCGGTAAATTCGCTCTCCGGACGGCTGATAACCGTTGGCGTACCTTTATCGAGATCCTCACGTAAGga < 1:4632003/71‑1 (MQ=255) gCGGTAAATTCGCTCTCCGGACGGCTGATAACCGTTGGCGTACCTTTATCGAGATCCTCACATAAGGAGAt < 1:4362927/71‑1 (MQ=255) gTAAATTCGCTCTCCGGACGGCTGATAACCGTTGGCGTACCTTTATCGAGATCCTCACGTAAGGAGAtgtg < 1:2348134/71‑1 (MQ=255) tAAATTCGCTCTCCGGACGGCTGATAACCGTTGGCGTACCTTTATCGAGATCCTCACGTAAGGAGAtgtg > 1:121221/1‑70 (MQ=255) tAAATTCGCTCTCCGGACGGCTGATAACCGTTGGCGTACCTTTATCGAGATCCTCACGTAAGGAGATgtgt < 1:4211215/71‑1 (MQ=255) aaTTCGCTCTCCGGACGGCTGATAACCGTTGGCGTACCTTTATCGAGATCCTCACGTAAGGAGATGTGTaa > 1:4260743/1‑71 (MQ=255) aaTTCGCTCTCCGGACGGCTGATAACCGTTGGCGTACCTTTATCGAGATCCTCACATAAGGAGATGTGTaa > 1:4178969/1‑71 (MQ=255) aaTTCGCTCTCCGGACGGCTGATAACCGTTGGCGTACCTTTATCGAGATACTCACGTAAGGAGATGTGTaa > 1:773924/1‑71 (MQ=255) ttCGCTCTCCGGACGGCTGATAACCGTTGGCGTACCTTTATCGAGATCCTCACGTAAGgag < 1:2041032/61‑1 (MQ=255) ttCGCTCTCCGGACGGCTGATAACCGTTGGCGTACCTTTATCGAGATCCTCACGTAAGGAGATGTGTAACg > 1:3896587/1‑71 (MQ=255) cGCTCTCCGGACGGCTGATAACCGTTGGCGTACCTTTATCGAGATCCTCACGTaa > 1:576038/1‑55 (MQ=255) cGCTCTCCGGACGGCTGATAACCGTTGGCGTACCTTTATCGAGATCCTCACGTaa > 1:4176875/1‑55 (MQ=255) cGCTCTCCGGACGGCTGATAACCGTTGGCGTACCTTTATCGAGATCCTCACGTAAGgaga > 1:2791907/1‑60 (MQ=255) cGCTCTCCGGACGGCTGATAACCGTTGGCGTACCTTTATCGAGATCCTCACGTAAGgaga > 1:186366/1‑60 (MQ=255) gCTCTCCGGACGGCTGATAACCGTTGGCGTACCTTTATCGAGATCCTCACGTAAGGAGATGTGTAACGGc < 1:2423495/70‑1 (MQ=255) gCTCTCCGGACGGCTGATAACCGTTGGCGTACCTTTATCGAGATCCTCACGTAAGGAGATGTGTAACGGc < 1:2428791/70‑1 (MQ=255) gCTCTCCGGACGGCTGATAACCGTTGGCGTACCTTTATCGAGATCCTCACGTAAGGAGATGTGTAACGGc < 1:994516/70‑1 (MQ=255) gCTCTCCGGACGGCTGATAACCGTTGGCGTACCTTTATCGAGATCCTCACGTAAGGAGATGTGTAACGGc < 1:273272/70‑1 (MQ=255) ctcCGGACGGCTGATAACCGTTGGCGTACCTTTATCGAGATCCTCACGTAAGGAGATGTGTaa > 1:4439487/1‑63 (MQ=255) tcCGGACGGCTGATAACCGTTGGCGTACCTTTATCGAGATCCTCACGt > 1:819429/1‑48 (MQ=255) tcCGGACGGCTGATAACCGTTGGCGTACCTTTATCGAGATCCTCACGTa < 1:1073957/49‑1 (MQ=255) tcCGGACGGCTGATAACCGTTGGCGTACCTTTATCGAGATCCTCACGTAAGGAGATGTGTaa < 1:2298962/62‑1 (MQ=255) tcCGGACGGCTGATAACCGTTGGCGTACCTTTATCGAGATCCTCACGTAAGGAGATGTGTAACGGCATCTg > 1:2363819/1‑71 (MQ=255) cGGACGGCTGATAACCGTTGGCGTACCTTTATCGAGATCCTCACGTAAGGAGATGTGTAACGGCATCt < 1:2633601/68‑1 (MQ=255) aCGGCTGATAACCGTTGGCGTACCTTTATCGAGATCCTCACGTAAGGAGATGTGTAACGGCATCTGccc > 1:997642/1‑69 (MQ=255) cGGCTGATAACCGTTGGCGTACCTTTATCGAGATCCTCACGTAAGGAGATGTGTAACGGCATCTGc > 1:1621628/1‑66 (MQ=255) gCTGATAACCGTTGGCGTACCTTTATCGAGATCCTCACGTAAg < 1:4085381/43‑1 (MQ=255) tGATAACCGTTGGCGTACCTTTATCGAGATCCTCACGTAAGGAGATgtgt < 1:2171523/50‑1 (MQ=255) gATAACCGTTGGCGTACCTTTATCGAGATCCTCACGTAAGGAGATGTGTAACGGCATCTGCCCCAGCATCt > 1:2700281/1‑71 (MQ=255) tAACCGTTGGCGTACCTTTATCGAGATCCTCACGTAAGGAGATGTGTAACgg < 1:2199237/52‑1 (MQ=255) tAACCGTTGGCGTACCTTTATCGAGATCCTCACGTAAGGAGATGTGTAACGGCATCTGCCCCAGCAGCtgt > 1:4298727/1‑71 (MQ=255) aaCCGTTGGCGTACCTTTATCGAGATCCTCACGTAAGGAGATGAGTAACGGCATCTGCCCCAGCAGCtgtg > 1:3753104/1‑71 (MQ=255) aCCGTTGGCGTACCTTTATCGAGATCCTCACGTAAGgaga > 1:2942511/1‑40 (MQ=255) aCCGTTGGCGTACCTTTATCGAGATCCTCACGTAAGgaga > 1:3015376/1‑40 (MQ=255) aCCGTTGGCGTACCTTTATCGAGATCCTCACGTAAGGAGATGTGTAACGGCATCTGCCCCAGCAGCtgtg < 1:438791/70‑1 (MQ=255) aCCGTTGGCGTACCTTTATCGAGATCCTCACGTAAGGAGATGTGTAACGGCATCTGCCCCAGCAGCtgtg < 1:43229/70‑1 (MQ=255) aCCGTTGGCGTACCTTTATCGAGATCCTCACGTAAGGAGATGTGTAACGGCATCTGCCCCAGCAGCtgtg < 1:3676893/70‑1 (MQ=255) ccGTTGGCGTACCTTTATCGAGATCCTCACATAAGga < 1:1843331/37‑1 (MQ=255) ccGTTGGCGTACCTTTATCGAGATCCTCACATAAGga < 1:3750789/37‑1 (MQ=255) cGTTGGCGTACCTTTATCGAGATCCTCTCGTAAGGAGATGTGTAACGGCATCTGCCCCAGCAGCTGTGTa < 1:3136649/70‑1 (MQ=255) gTTGGCGTACCTTTATCGAGATCCTCACGTAAGGAGATGTGTaa > 1:380387/1‑44 (MQ=255) tGGCGTACCTTTATCGAGATCCTCACATAAGGAGATGTGTAACGGCATc > 1:2293542/1‑49 (MQ=255) gCGTACCTTTATCGAGATCCTCACGTAAGGAGATGTGTAACGGCATCTGCCCCAGCAGCTGTGTATGGTa < 1:2157502/70‑1 (MQ=255) gCGTACCTTTATCGAGATCCTCACGTAAGGAGATGTGTAACGGCATCTGCCCCAGCAGCTGTGTATGGTa < 1:2172023/70‑1 (MQ=255) gTACCTTTATCGAGATCCTCACGTAAGGAGATGTGTAACGGCATCTGCCCCAGCAGCTGTGTATGGTAttt > 1:4046625/1‑71 (MQ=255) tACCTTTATCGAGATCCTCACGTAAGGAGATGTGTAACGGCATCTGCCCCAGCAGCTGTGTATGGTAtttt < 1:3955467/71‑1 (MQ=255) tACCTTTATCGAGATCCTCACATAAGGAGATGTGTAACGGCATCTGCCCCAGCAGCTGTGTATGGTAtttt > 1:2114159/1‑71 (MQ=255) aCCTTTATCGAGATCCTCACGTAAGGAGATGTGTAACGGCATCTGCCCCAGCAGCTGTGTa > 1:2697535/1‑61 (MQ=255) aCCTTTATCGAGATCCTCACGTAAGGAGATGTGTAACGGCATCTGCCCCAGCAGCTGTGTa > 1:2498894/1‑61 (MQ=255) ccTTTATCGAGATCCTCACGTAAGGAGATGTGTAACGGCATCTGCCCCAGCAGCTGTGTATGGTAttttt > 1:2886350/1‑70 (MQ=255) ccTTTATCGAGATCCTCACATAAGGAGATGTGTAACGGCATCTGCCCca > 1:11512/1‑49 (MQ=255) ccTTTATCGAGATCCTCACATAAGGAGATGTGTAACGGCATCTGCCCca > 1:691529/1‑49 (MQ=255) cTTTATAGAGATCCTCACGTAAGGAGATGTGTAACGGCa < 1:4469933/39‑1 (MQ=255) ttATCGAGATCCTCACGTAAGGAGATGTGTAACGGCATc > 1:1904949/1‑39 (MQ=255) ttATCGAGATCCTCACGTAAGGAGATGTGTAACGGCATCTGCCCCAGCAGCTGTGTATGGTATTTTTCAGc > 1:3768085/1‑71 (MQ=255) tATCGAGATCCTCACGTAAGGAGATGTGTAACGGCATCTGc > 1:68690/1‑41 (MQ=255) tATCGAGATCCTCACGTAAGGAGATGTGTAACGGCATCTGc > 1:2928824/1‑41 (MQ=255) cGAGATCCTCACATAAGGAGATGTGTAACGGCATCTGCCCCAGCAGCTGTGTATgg > 1:3238520/1‑56 (MQ=255) gaTCCTCACGTAAGGAGATGTGTAACGGCATCTGCCCCAGCAGCTGTGTATGGTATTTTTCAGCCAGttt < 1:462317/70‑1 (MQ=255) aTCCTCACGTAAGGAGATGTGTAACGGGATCTGCCCCAGCAGCtgtg < 1:3193831/47‑1 (MQ=255) aTCCTCACGTAAGGAGATGTGTAACGGCATCTGCCCcagcag < 1:1764269/42‑1 (MQ=255) cTCACGTAAGGAGATGTGTAACGGCATCTGCCCCAGCAGCTGTGTATGGTATTTTTCAGCCAGTTTCTCTg < 1:4158275/71‑1 (MQ=255) cTCACGTAAGGAGATGTGTAACGGCATCTGCCCCAGCAGCTGTGTATGGTATTTTTCAGCCAGTTTCTCTg < 1:1498376/71‑1 (MQ=255) cACGTAAGGAGATGTGTAACGGCATCTGCCCCAGCAGCTGTGTATGGTATTTTTCAGCCAGTTTCTCTg > 1:495245/1‑69 (MQ=255) | CGGTAGATAGCGGTAAATTCGCTCTCCGGACGGCTGATAACCGTTGGCGTACCTTTATCGAGATCCTCACGTAAGGAGATGTGTAACGGCATCTGCCCCAGCAGCTGTGTATGGTATTTTTCAGCCAGTTTCTCTG > W3110S.gb/2196475‑2196610 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |