| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,197,153 | C→T | 27.3% | G117D (GGC→GAC) | mrp ← | antiporter inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,197,153 | 0 | C | T | 27.3% | 52.6 / 20.0 | 33 | G117D (GGC→GAC) | mrp | antiporter inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (15/9); new base T (3/6); total (18/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCTTCTGCCGCCAGCGCCAGTGCCAGGTTTACCGCCGTGGACGATTTACCCACGCCGCCTTTACCGGAACTCACCGCGATAATGTTCTTCACACCGTTAATTCCCGGCTGATTCTTCACGCGTTTCAG > W3110S.gb/2197096‑2197223 | ccTTCTGCCGCCAGCGCCAGTGCCAGGTTTACCGCCGTGGACGATTTACCCACGCCGCCTTTACCGGAAc > 1:2303928/1‑70 (MQ=255) cTTCTGCCGCCAGCGCCAGTGCCAGGTTTACCGCCGTGGACGATTTACCCACGCCGTCTTTACCGGAACTc < 1:541921/71‑1 (MQ=255) cTTCTGCCGCCAGCGCCAGTGCCAGGTTTACCGCCGTGGACGATTTACCCACGCCGCCTTTACCgg < 1:921762/66‑1 (MQ=255) cTTCTGCCGCCAGCGCCAGTGCCAGGTTTACCGCCGTGGACGATTTACCCACGCCGCCTTTACCGGAACt < 1:2672502/70‑1 (MQ=255) cgccagcgccagTGCCAGGTTTACCGCCGTGGACGATTTACCCACGCCGTCTTTACCGGAACTCACCGCGa < 1:462430/71‑1 (MQ=255) ccagcgccagTGCCAGGTTTACCGCCGTGGACGATTTACCCACGCCGCCTTTACCGGAACTCACCGCGAt < 1:516879/70‑1 (MQ=255) gcgccagTGCCAGGTTTACCGCCGTGGACGATTTACCCACGCCGCCTTTACCgg < 1:2567018/54‑1 (MQ=255) ccAGGTTTACCGCCGTGGACGATTTACCCACGCCGTCTTTACCGGAACTCACCGCGAt < 1:4060897/58‑1 (MQ=255) ggTTTACCGCCGTGGACGATTTACCCCCGCCGCCTTTACCGGa > 1:2098488/1‑43 (MQ=255) gTTTACCGCCGTGGACGATTTACCCACGCCGTCTTTACCGGAACTCACcgc < 1:3264548/51‑1 (MQ=255) tttACCGCCGTGGACGATTTACCCACGCCGCCTTTACCGGAACt > 1:2651742/1‑44 (MQ=255) tttACCGCCGTGGACGATTTACCCACGCCGCCTTTACCGGAACt > 1:1692802/1‑44 (MQ=255) ttACCGCCGTGGACGATTTACCCACGCCGTCTTTACCGGAACTCACCGCGATAATGTTCTTCACACCGtt > 1:2487028/1‑70 (MQ=255) ttACCGCCGTGGACGATTTACCCACGCCGCCTTTACCGGAACTCACCGCGATaa > 1:1308658/1‑54 (MQ=255) ttACCGCCGTGGACGATTTACCCACGCCGCCGTTACCGGaa > 1:2175693/1‑41 (MQ=255) gccgTGGACGATTTACCCACGCCGCCTTTACCGGAACTCACCGCGATAATGTTCTTCACACCGTTAATTcc < 1:3754979/71‑1 (MQ=255) ccgTGGACGATTTACCCACGCCGCCTTTACCGGAACTCACCGCGATAATGTTCTTCACAcc < 1:1651491/61‑1 (MQ=255) gTGGACGATTTACCCACGCCGTCTTTACCGGAACTCa > 1:1570606/1‑37 (MQ=255) ttgACGATTTACCCACGCCGTCTTTACCGGAACTCACCGCGATaa < 1:2290855/43‑1 (MQ=38) gACGATTTACCCACGCCGCCTTTACCGGAACTCAc > 1:3122601/1‑35 (MQ=255) gACGATTTACCCACGCCGCCTTTACCGGAACTCACcgc > 1:3571085/1‑38 (MQ=255) aTTTACCCACGCCGTCTTTACCGGAACTCACCGCGATAATGTTCTTCACACCGTTAATTCCCGGCTGAttc < 1:1877578/71‑1 (MQ=255) ttACCCACGCCGTCTTTACCGGAACTCACCGCGATAATGttc > 1:3876491/1‑42 (MQ=255) ttACCCACGCCGCCTTTACCGGAACTCACCGCGATaa > 1:2663029/1‑37 (MQ=255) ttACCCACGCCGCCTTTACCGGAACTCACCGCGATAATGttct > 1:143322/1‑43 (MQ=255) ttACCCACGCCGCCTTTACCGGAACTCACCGCGATAATGTTCTTCACACCGTTaa > 1:805782/1‑55 (MQ=255) tACCCACGCCGCCTTTACCGGAACTCACCGCGATAATGttctt > 1:1455529/1‑43 (MQ=255) tACCCACGCCGCCTTTACCGGAACTCACCGCGATAATGTTCTTCACACCGTTAATTCCCGGCTGAttctt < 1:2938486/70‑1 (MQ=255) tACCCACGCCGCCTTTACCGGAACTCACCGCGATAATGTTCTTCACACCGTTAATTCCCGGCTGAttctt > 1:3943588/1‑70 (MQ=255) tACCCACGCCGCCTTTACCGGAACTCACCGCGATAATGTTCTTCACACCGTTAATTCCCGGCTGAttctt < 1:802110/70‑1 (MQ=255) tACCCACGCCGCCTTTACCGGAACTCACCGCGATAATGTTCTTCACACCGTTAATTCCCGGCTGAttctt > 1:1659222/1‑70 (MQ=255) cccACGCCGCCTTTACCGGAACTCACCGCGATAATGTTCTTCACACCGTTa < 1:941938/51‑1 (MQ=255) aCGCCGCCTTTACCGGAACTCACCGCGATAATGTTCTTCACACCGTTAATTCCCGGCTGATTCTTCAcgcg > 1:3539617/1‑71 (MQ=255) ccgtcTTTACCGGAACTCACCGCGATAATGTTCTTCACACCGTTAATTCCCGGCTGATTCTTCACGCGttt > 1:1225466/1‑71 (MQ=255) ccgccTTTACCGGAACTCACCGCGATAATGTTCTTcaca > 1:2606812/1‑39 (MQ=255) ccgccTTTACCGGAACTCACCGCGATAATGTTCTTcaca > 1:1387442/1‑39 (MQ=255) ccgccTTTACCGGAACTCACCGCGATAATGTTCTTCACACCGTTaa < 1:1672200/46‑1 (MQ=255) gccTTTACCGGAACTCACCGCGATAATGTTCTTCACACCGTTAATTCCCGGCTGATTCTTCACGCGttt > 1:2420938/1‑69 (MQ=255) ccTTTACCGGAACTCACCGCGATAATGTTCTTCACACCGTTAATTCCCGGCTGATTCTTCACGCGTTTCag < 1:2624236/71‑1 (MQ=255) | CCTTCTGCCGCCAGCGCCAGTGCCAGGTTTACCGCCGTGGACGATTTACCCACGCCGCCTTTACCGGAACTCACCGCGATAATGTTCTTCACACCGTTAATTCCCGGCTGATTCTTCACGCGTTTCAG > W3110S.gb/2197096‑2197223 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |