| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 72,433 | A→G | 8.6% | R165R (CGT→CGC) | thiQ ← | thiamin transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 72,433 | 0 | A | G | 8.6% | 149.7 / 6.5 | 58 | R165R (CGT→CGC) | thiQ | thiamin transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (34/19); new base G (2/3); total (36/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.22e-01 | |||||||||||
TGCGACACCATCAATAGCGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAACATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGTACCAGACAAC > W3110S.gb/72365‑72503 | tgCGACACCATCAATAGCGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAACATCTCCTGACg < 1:4223655/71‑1 (MQ=255) acCATCAATAGCGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAACATCTCCTGACGTAACGc > 1:3777371/1‑71 (MQ=255) aTCAATAGCGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAACATCTCCTGACGTAACGCCg > 1:3944458/1‑70 (MQ=255) aaTAGCGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAACATCTCCTGGCGTAACGCCGGATc > 1:1120134/1‑71 (MQ=255) aaTAGCGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAACATCTCCTGACGTAACGCCGGATc > 1:3159930/1‑71 (MQ=255) aGCGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAACATCTCCTGACGTAACGCCGGATCGAg > 1:1216500/1‑71 (MQ=255) gCGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAACATCTCCTGACGTAACGCCGGATCGAgc > 1:3496626/1‑71 (MQ=255) cGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAACATCTCCTGACGTAACGCCGGATCGAgcg < 1:3557771/71‑1 (MQ=255) gTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAACATCTCCTGACGTAACGCCGGATCGAgcgc < 1:3058750/71‑1 (MQ=255) tCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAACATCTCCTGACGTAACGCCGGATCGAGCGCa < 1:4124806/71‑1 (MQ=255) cATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAACATCTCCTGACGTAACGCCGGATCGAgcg > 1:156303/1‑68 (MQ=255) aTTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAACATCTCCTGACGTaa > 1:1278225/1‑53 (MQ=255) aTTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAACATCTCCTGACGTAACGCCGGATCGAGCGCa > 1:3621453/1‑69 (MQ=255) tttGCTGCTGGCAGCTCGTGCTCACCAGCGTCAACATCTCCTGACGTAACGCCg > 1:394458/1‑54 (MQ=255) tCGTGCTCACCAGCGTCAACATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCaa > 1:3869715/1‑70 (MQ=255) tCGTGCTCACCAGCGTCAACATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCaa > 1:3788120/1‑70 (MQ=255) gTGCTCACCAGCGTCAACATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATaa < 1:4085840/71‑1 (MQ=255) cTCACCAGCGTCAACATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATaaaa < 1:421986/70‑1 (MQ=255) cTCACCAGCGTCAACATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAAt > 1:3051519/1‑71 (MQ=255) cACCAGCGTCAACATCTCGTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATaaa > 1:4621806/1‑67 (MQ=255) cACCAGCGTCAACATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATaaa > 1:268221/1‑67 (MQ=255) cACCAGCGTCAACATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATaaa > 1:2096520/1‑67 (MQ=255) aCCAGCGTCAACATCTCCTGGCGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCg < 1:2303211/70‑1 (MQ=255) aCCAGCGTCAACATCTCCTGGCGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCg < 1:528611/70‑1 (MQ=255) aCCAGCGTCAACATCTCCTGGCGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCg < 1:3890783/70‑1 (MQ=255) aGCGTCAACATCTCCTGGCGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATaaaa > 1:1975062/1‑64 (MQ=255) aGCGTCAACATCTCCTGACGTAACGCCGGATCGAGCGCagag < 1:1550832/42‑1 (MQ=255) aGCGTCAACATCTCCTGACGTAACGCCGGATCGAGCGCagag < 1:3250588/42‑1 (MQ=255) aGCGTCAACATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTg < 1:4050771/71‑1 (MQ=255) gTCAACATCTCCTGACGTAACGCCGGATCGAGCGCAGAGACCGGTTCATCGAGCAATAAAATCGGCTGTTc > 1:3560726/1‑71 (MQ=255) tCAACATCTCCTGACGTAACGCCGGATCGAGCGCagag < 1:2006627/38‑1 (MQ=255) tCAACATCTCCTGACGTAACGCCGGATCGAGCGCagag < 1:2155478/38‑1 (MQ=255) cAACATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATaaa < 1:7702/58‑1 (MQ=255) aaCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGtt > 1:3001641/1‑67 (MQ=255) aaCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTcgcg > 1:3393610/1‑71 (MQ=255) aaCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTcgcg > 1:2400230/1‑71 (MQ=255) aaCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTcgcg > 1:4529395/1‑71 (MQ=255) aaCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTcgcg < 1:3153430/71‑1 (MQ=255) aaCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTcgcg > 1:1575183/1‑71 (MQ=255) aaCATCTCCTGACGTAACGCCGGATCGAGCCCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTcgcg > 1:4256426/1‑71 (MQ=255) aCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTcgcg < 1:3525865/70‑1 (MQ=255) aTCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGtt > 1:1447705/1‑40 (MQ=255) aTCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCAt > 1:2075915/1‑43 (MQ=255) aTCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGTAc > 1:3666307/1‑71 (MQ=255) ctcCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTcgcg > 1:2267941/1‑66 (MQ=255) ctcCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGt > 1:3304705/1‑67 (MQ=255) ctcCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGTAc > 1:869486/1‑69 (MQ=255) tcCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGTAc > 1:4124488/1‑68 (MQ=255) ttGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGTACCAGa > 1:1209971/2‑70 (MQ=255) cTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGTACCa < 1:1352313/68‑1 (MQ=255) cTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGTACCAGAc < 1:4425659/71‑1 (MQ=255) cTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGTACCAGAc < 1:4500690/71‑1 (MQ=255) tGACGTAACGCCGGATCGAGCGCAGAGAACGGTTc < 1:3502740/35‑1 (MQ=255) tGACGTAACGCCGGATCGAGCGCAGAGAACGGTTc < 1:3301700/35‑1 (MQ=255) tGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCTTACCAGAc > 1:1929027/1‑70 (MQ=255) tGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGTACCAGAc > 1:3467945/1‑70 (MQ=255) gACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGTACCAGACaa > 1:3807570/1‑71 (MQ=255) gACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGTACCAGACaa > 1:1259765/1‑71 (MQ=255) aCGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAAt > 1:1233738/1‑48 (MQ=255) aCGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGTACCAGACAAc < 1:1241885/71‑1 (MQ=255) | TGCGACACCATCAATAGCGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAACATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGTACCAGACAAC > W3110S.gb/72365‑72503 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |