| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,291,258 | C→T | 23.4% | G326D (GGC→GAC) | yejO ← | predicted autotransporter outer membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,291,258 | 0 | C | T | 23.4% | 152.6 / 43.0 | 77 | G326D (GGC→GAC) | yejO | predicted autotransporter outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (28/31); new base T (6/12); total (34/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATAATTTATTTGCGGCTTCATGACCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAGGAATACGTGA > W3110S.gb/2291188‑2291327 | aTAATTTATTTGCGGCTTCATGACCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGc > 1:4045432/1‑71 (MQ=255) aaTTTATTTGCGGCTTCATGACCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGc > 1:4328899/1‑69 (MQ=255) atttatttGCGGCTTCATGACCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAg > 1:3256631/1‑71 (MQ=255) atttatttGCGGCTTCATGACCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAg > 1:2587983/1‑71 (MQ=255) ttatttGCGGCTTCATGACCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGcc > 1:2045718/1‑71 (MQ=255) tttGCGGCTTCATGACCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCa < 1:3576583/70‑1 (MQ=255) tttGCGGCTTCATGACCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCa < 1:4601644/70‑1 (MQ=255) gCTTCATGACCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGAc < 1:1453312/67‑1 (MQ=255) cTTCATGACCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAg < 1:709/71‑1 (MQ=255) tCATGACCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAgcg > 1:408303/1‑71 (MQ=255) tCATGACCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAgcg > 1:2160692/1‑71 (MQ=255) cATGACCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAgcgc > 1:1201610/1‑71 (MQ=255) tGACCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGTCAGCCCa < 1:1346626/58‑1 (MQ=255) aCCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGcc < 1:4313123/71‑1 (MQ=255) aCCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGc > 1:4081959/1‑70 (MQ=255) aCCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGc < 1:3446055/70‑1 (MQ=255) aCCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGc < 1:1879775/70‑1 (MQ=255) cTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGtt < 1:1434345/60‑1 (MQ=255) cTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCAt < 1:4151299/71‑1 (MQ=255) tCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGTCAg < 1:1668066/49‑1 (MQ=255) tCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGTCAg < 1:556394/49‑1 (MQ=255) aaGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGc > 1:2627038/1‑44 (MQ=255) aaGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCAttgt > 1:2593419/1‑71 (MQ=255) aaGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCAttgt > 1:2942508/1‑71 (MQ=255) aaGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGa > 1:2510234/1‑53 (MQ=255) aaGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCAttgt < 1:3861234/71‑1 (MQ=255) aaGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCAttgt > 1:2765253/1‑71 (MQ=255) aaGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCAtt < 1:1272775/69‑1 (MQ=255) aaGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCAtt < 1:528165/69‑1 (MQ=255) aTGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGa < 1:4218956/70‑1 (MQ=255) ccATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGa > 1:1801246/1‑46 (MQ=255) ccATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAAc > 1:1495918/1‑71 (MQ=255) catcatTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCAttgttg < 1:2670103/65‑1 (MQ=255) atcatTCCCTCTGACTGAAACGTTAACCATTGTGCCAGcc < 1:2793040/40‑1 (MQ=255) tcatTCCCTCTGACTGAAACGTTAACCATTGTGTCAGCCCAGACGtt < 1:3025314/47‑1 (MQ=255) tcatTCCCTCTGACTGAAACGTTAACCATTGTGTCAGCCCAGACGTTAGGGCGGCCATTGTTGATAACCat < 1:1645828/71‑1 (MQ=255) tcatTCCCTCTGACTGAAACGTTAACCATTGTGCCAGcc < 1:4237263/39‑1 (MQ=255) tcatTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTa < 1:2956005/48‑1 (MQ=255) tcatTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAgc < 1:4296432/50‑1 (MQ=255) tcatTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCAttg < 1:4332204/60‑1 (MQ=255) tcatTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCAttg < 1:98545/60‑1 (MQ=255) tcatTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCat < 1:1834119/71‑1 (MQ=255) catTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATaa > 1:4010711/1‑66 (MQ=255) catTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATaa > 1:3624197/1‑66 (MQ=255) atTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCa > 1:2911856/1‑39 (MQ=255) atTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCata > 1:895429/1‑70 (MQ=255) ttCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAg > 1:2846185/1‑46 (MQ=255) ttCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATAtt < 1:3472151/71‑1 (MQ=255) cccTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAg > 1:1730185/1‑44 (MQ=255) cccTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAg > 1:4213168/1‑44 (MQ=255) cccTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATAtttt > 1:3507751/1‑71 (MQ=255) cccTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATAtttt > 1:2316861/1‑71 (MQ=255) ccTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTc < 1:1861526/71‑1 (MQ=255) tctGACTGAAACGTTAACCATTGTGCCAGCCCAGACg < 1:4331634/37‑1 (MQ=255) ctgactgaAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCa > 1:2705237/1‑69 (MQ=255) tgactgaAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCa > 1:1816351/1‑68 (MQ=255) tgactgaAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCa > 1:2396941/1‑68 (MQ=255) actgaAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCaga < 1:4490949/68‑1 (MQ=255) actgaAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCaga < 1:236632/68‑1 (MQ=255) actgaAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCaga < 1:2640029/68‑1 (MQ=255) actgaAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCaga < 1:3394212/68‑1 (MQ=255) ctgaAACGTTAACCATTGTGCCAGCCCAGACGTTAGcgcg < 1:1041205/40‑1 (MQ=255) ctgaAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATg < 1:3579630/71‑1 (MQ=255) tgaAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCAttgttg < 1:602510/49‑1 (MQ=255) gaAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGc > 1:3054729/1‑40 (MQ=255) gaAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATg > 1:1668584/1‑69 (MQ=255) gaAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATg > 1:3500197/1‑69 (MQ=255) cGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCtttt < 1:3223245/71‑1 (MQ=255) ttAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCtttt < 1:4180700/69‑1 (MQ=255) ttAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCtttt > 1:2509442/1‑69 (MQ=255) aaCCATTGTGTCAGCCCAGACGTTAGCGCGGCCATTGTTGATa > 1:4234835/1‑43 (MQ=255) aaCCATTGTGTCAGCCCAGACGTTAGCGCGGCCATTGTTGATa > 1:1595324/1‑43 (MQ=255) aaCCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCa < 1:4607376/55‑1 (MQ=255) aCCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAgg < 1:4478488/70‑1 (MQ=255) aCCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAgg > 1:148214/1‑70 (MQ=255) aCCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAGGa < 1:3257122/71‑1 (MQ=255) cATTGTGCCAGCCCAGACGTTAGCGCGGCCAttgttg > 1:3485328/1‑37 (MQ=255) aTTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAGGAATa < 1:3807033/71‑1 (MQ=255) gtgCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCaga > 1:1556226/1‑50 (MQ=255) tgCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAGGAATACGt < 1:1006008/70‑1 (MQ=255) ccAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAGGAATACGTGa < 1:217784/70‑1 (MQ=255) | ATAATTTATTTGCGGCTTCATGACCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAGGAATACGTGA > W3110S.gb/2291188‑2291327 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |