| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,329,422 | A→T | 28.2% | D215V (GAT→GTT) | atoA → | acetyl‑CoA:acetoacetyl‑CoA transferase, beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,329,422 | 0 | A | T | 28.2% | 85.0 / 38.5 | 53 | D215V (GAT→GTT) | atoA | acetyl‑CoA:acetoacetyl‑CoA transferase, beta subunit |
| Reads supporting (aligned to +/- strand): ref base A (21/17); new base T (6/9); total (27/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
GCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTCGCCGCCGATCTGAATACGCAACGGGGTGATTTATGATTGGTCGCATATCGCGTTTTATGACGCGTTTTGTCAGCCGGTGGCTTCCCGATCCACTGATCT > W3110S.gb/2329355‑2329492 | gCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTCGCCGCCGATCTGAATACGCAACGGGGTGAtt < 1:1268650/70‑1 (MQ=255) cACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTCGCCGCCGATCTGAATACGCAACGGGGTGATTTAt < 1:552132/71‑1 (MQ=255) gtgcgtgcCAAAACAGAAGCTCGGTTTGAAGTCGCCGCCGATCTGAATACGCAACGGGGTGATTTATg > 1:2993008/1‑68 (MQ=255) aCAGAAGCTCGGTTTGAAGTCGCCGCCGATCTGAATACGCAACGGGGTGTTTTATGATTGGTCGCATATcg > 1:100639/1‑71 (MQ=255) cAGAAGCTCGGTTTGAAGTCGCCGCCGATCTGAATACGCAACGGGGTGTTTTATGATTg < 1:2532780/59‑1 (MQ=255) cAGAAGCTCGGTTTGAAGTCGCCGCCGATCTGAATACGCAACGGGGTGTTTTATGATTg < 1:205956/59‑1 (MQ=255) aGAAGCTCGGTTTGAAGTCGCCGCCGATCTGAATACGCAACGGGGTGATTTATGATTGGTCGCATATcgcg > 1:3385672/1‑71 (MQ=255) aGAAGCTCGGTTTGAAGTCGCCGCCGATCTGAATACGCAACGGGGTGATTTATGATTGGTCGCATATcgcg > 1:2584782/1‑71 (MQ=255) aGCTCGGTTTGAAGTCGCCGCCGATCTGAATACGCAACGGGGTGTTTTATGATTGGTCGCATATCGCGttt < 1:735964/71‑1 (MQ=255) tCGGTTTGAAGTCGCCGCCGATCTGAATACGCAACGGGGTGAttt < 1:1594231/45‑1 (MQ=255) ttGAAGTCGCCGCCGATCTGAATACGCAACGGGGTGATTTATGATTGGTCGCATATCGCGTTTTATGAc > 1:1165444/1‑69 (MQ=255) tGAAGTCGCCGCCGATCTGAATACGCAACGGGGTGATTTAt < 1:1947709/41‑1 (MQ=255) aaGTCGCCGCCGATCTGAATACGCAACGGGGTGTTTTATGATTGGTCGCATATCGCGTTTTATGACGCGtt > 1:2905973/1‑71 (MQ=255) aaGTCGCCGCCGATCTGAATACGCAACGGGGTGATTTATGATTGGTCGCATATCGCGTTTTa > 1:3238898/1‑62 (MQ=255) gTCGCCGCCGATCTGAATACGCAACGGGGTGTTTTATGATTGGTCGCATATCGCGTTTTATGACGCGtttt < 1:251152/71‑1 (MQ=255) tCGCCGCCGATCTGAATACGCAACGGGGTGATTTAt < 1:4428832/36‑1 (MQ=255) tCGCCGCCGATCTGAATACGCAACGGGGTGATTTATGATTg < 1:2494568/41‑1 (MQ=255) tCGCCGCCGATCTGAATACGCAACGGGGTGATTTATGATTGGTCGCATATCGCGTTTTATGACGCGTTTTg < 1:1634738/71‑1 (MQ=255) tCGCCGCCGATCTGAATACGCAACGGGGTGATTTATGATTGGTCGCATATCGCGTTTTATGACGCGTTTTg < 1:279056/71‑1 (MQ=255) gccgccGATCTGAATACGCAACGGGGTGTTTTATGATTGGTCGCATATCGCGTTTTATGACGCGTTTTGTc < 1:1186486/71‑1 (MQ=255) gccgccGATCTGAATACGCAACGGGGTGATTTATGa > 1:1172067/1‑36 (MQ=255) gccgccGATCTGAATACGCAACGGGGTGATTTATGATTGGTCGCata > 1:769511/1‑47 (MQ=255) gccgccGATCTGAATACGCAACGGGGTGATTTATGATTGGTCGCATATCGCGTTTTATGACGCGTTTTGt > 1:2578438/1‑70 (MQ=255) gccgccGATCTGAATACGCAACGGGGTGATTTATGATTGGTCGCATATCGCGTTTTATGACGCGTTTTGt > 1:1870181/1‑70 (MQ=255) gccgccGATCTGAATACGCAACGGGGTGATTTATGATTGGTCGCATATCGCGTTTTATGACGCGTTTTGt > 1:516741/1‑70 (MQ=255) gccgccGATCTGAATACGCAACGGGGTGATTTATGATTGGTCGCATATCGCGTTTTATGACGCGTTTTGt > 1:3229594/1‑70 (MQ=255) aTCTGAATACGCAACGGGGTGTTTTATGATTGGTCGCATATCGCGTTTTATGACGCGTTTTGt < 1:3379018/63‑1 (MQ=255) tCTGAATACGCAACGGGGTGTTTTATGATTGGTCGCATATCGCGttt < 1:135559/47‑1 (MQ=255) tCTGAATACGCAACGGGGTGATTTATGATTGGTCGCATATCGCGTTTTATGACGCGttt > 1:485167/1‑59 (MQ=255) cTGAATACGCAACGGGGTGTTTTATGATTGGTCGCATATCGCGTTTTATGACGCGTTTTGt < 1:283331/61‑1 (MQ=255) cTGAATACGCAACGGGGTGATTTATGATTGGTCGCATATCGCGTTTTATGACGCGtttt > 1:342006/1‑59 (MQ=255) cTGAATACGCAACGGGGTGATTTATGATTGGTCGCATATCGCGTTTTATGACGCGttt > 1:2452358/1‑58 (MQ=255) cTGAATACGCAACGGGGTGATTTATGATTGGTCGCATATCGCGTTTTATGACGCGTTTTGTCAGCCgg > 1:2665056/1‑68 (MQ=255) tGAATACGCAACGGGGTGATTTATGATTGGTCGCATATCGCGTTTTa < 1:1293845/47‑1 (MQ=255) tGAATACGCAACGGGGTGATTTATGATTGGTCGCATATCGCGTTTTATGACGCGtttt < 1:1791963/58‑1 (MQ=255) aaTACGCAACGGGGTGATTTATGATTGGTCGCATATCGCGTTTTATg > 1:1415912/1‑47 (MQ=255) aaTACGCAACGGGGTGATTTATGATTGGTCGCATATCGCGTTTTATGACGCGtt > 1:2413849/1‑54 (MQ=255) aaTACGCAACGGGGTGATTTATGATTGGTCGCATATCGCGTTTTATGACGCGTTTTGTCAGCCGGTGGCtt > 1:851314/1‑71 (MQ=255) aaTACGCAACGGGGTGATTTATGATTGGTCGCATATCGCGTTTTATGACGCGTTTTGTCAGCCGGTGGCtt > 1:845834/1‑71 (MQ=255) aaTACGCAACGGGGTGATTTATGATTGGTCGCATATCGCGTTTTATGACGCGTTTTGTCAGCCGGTGGCtt < 1:1566094/71‑1 (MQ=255) aTACGCAACGGGGTGATTTATGATTGGTCGCATATCGCGTTTTATGACGCGTTTTGTCAGCCGGTGGCTTc > 1:804530/1‑71 (MQ=255) aTACGCAACGGGGTGATTTATGATTGGTCGCATATCGCGTTTTATGACGCGTTTTGTCAGCCGGTGGCTTc > 1:3121256/1‑71 (MQ=255) aaCGGGGTGTTTTATGATTGGTCGCATATCGCGTTTTATGACGCGtttt < 1:1288776/49‑1 (MQ=255) aaCGGGGTGTTTTATGATTGGTCGCATATCGCGTTTTATGACGCGTTTTGTCAGCCGGTGGCTTCCCGATc > 1:625668/1‑71 (MQ=255) aaCGGGGTGATTTATGATTGGTCGCATATCGCGTTTTATGACGCGTTTTGTCAGCCGGTGGCTTCCCGATc < 1:2793863/71‑1 (MQ=255) aaCGGGGTGATTTATGATTGGTCGCATATCGCGTTTTATGACGCGTTTTGTCAGCCGGTGGCTTCCCGATc < 1:730325/71‑1 (MQ=255) ggggTGTTTTATGATTGGTCGCATATCGCGTTTTATg > 1:4637119/1‑37 (MQ=255) ggggTGTTTTATGATTGGTCGCATATCGCGTTTTATGa > 1:560585/1‑38 (MQ=255) ggggTGTTTTATGATTGGTCGCATATCGCGTTTTATGa > 1:2873546/1‑38 (MQ=255) gggTGATTTATGATTGGTGGCATATCGCGTTTTATGACGCGTTTTGTCAGcc < 1:3337369/52‑1 (MQ=255) ggTGATTTATGATTGGTCGCATATCGCGTTTTATGACGCGTTTTGTCAGCCGGTGGCTTCCCGATCCACTg < 1:4595280/71‑1 (MQ=255) tGATTTATGATTGGTCGCATATCGCGTTTTATGACGCGTTTTGTCAGCCGGTg < 1:62500/53‑1 (MQ=255) tGATTTATGATTGGTCGCATATCGCGTTTTATGACGCGTTTTGTCAGCCGGTGGCTTCCCGATCCACTg < 1:37990/69‑1 (MQ=255) aTTTATGATTGGTCGCATATCGCGTTTTATGACGCGttt > 1:3467010/1‑39 (MQ=255) aTTTATGATTGGTCGCATATCGCGTTTTATGACGCGTTTTGTCAGCCGGTGGc > 1:3621458/1‑53 (MQ=255) aTTTATGATTGGTCGCATATCGCGTTTTATGACGCGTTTTGTCAGCCGGTGGCTTCCCGATCCTCTGATCt > 1:3692154/1‑71 (MQ=255) aTTTATGATTGGTCGCATATCGCGTTTTATGACGCGTTTTGTCAGCCGGTGGCTTCCCGATCCACTGAt > 1:3679512/1‑69 (MQ=255) | GCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTCGCCGCCGATCTGAATACGCAACGGGGTGATTTATGATTGGTCGCATATCGCGTTTTATGACGCGTTTTGTCAGCCGGTGGCTTCCCGATCCACTGATCT > W3110S.gb/2329355‑2329492 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |