| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,394,072 | A→G | 9.2% | D97G (GAT→GGT) | yfbP → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,394,072 | 0 | A | G | 9.2% | 139.4 / 6.7 | 54 | D97G (GAT→GGT) | yfbP | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (22/27); new base G (2/3); total (24/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01 | |||||||||||
AAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGATCAAGACTTTGCAGCAAATCTCTTCCGTGTTGCTCGGAAATGTCTTTCAACGGGAAGGCTTGATGACG > W3110S.gb/2394005‑2394140 | aagaagTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGATc > 1:1281151/1‑70 (MQ=255) gaagTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGATc > 1:703977/1‑68 (MQ=255) ttCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGATCAAGACtt < 1:3450027/71‑1 (MQ=255) tCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGATCAAGACttt > 1:1365290/1‑71 (MQ=255) cGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGAt > 1:336422/1‑61 (MQ=255) cGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGAt > 1:3798481/1‑61 (MQ=255) cGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGATCAAGACTTTg > 1:2344375/1‑71 (MQ=255) gaagaaTGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGGTCAAGACTTTgc < 1:1578429/71‑1 (MQ=255) agaaTGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGATCAAGACTTTgcag > 1:4572798/1‑71 (MQ=255) gaaTGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGATCAAGACTTTgcagc < 1:553660/71‑1 (MQ=255) aTGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGATCAAGACTTTGCAGCaa < 1:1851223/71‑1 (MQ=255) aTGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGATCAAGACTTTGCAGCaa < 1:2143511/71‑1 (MQ=255) gCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGATCAAGACTTTGCAGCAAAt < 1:1470665/70‑1 (MQ=255) aGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGATCAAGACTTTGCAGCAAATCTCTTcc > 1:1541206/1‑71 (MQ=255) gTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGATCAAGACTTTGCAGCAAAtc < 1:3780568/64‑1 (MQ=255) gTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGATCAAGACTTTGCAGCAAATCTCTTCCg < 1:2692258/71‑1 (MQ=255) aaTGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGGTCAAGACTTTGCAGCaa < 1:4455085/58‑1 (MQ=255) aaTGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGATCAAGACTTTGCAGCAAATCTCTTCCgtgt < 1:3386429/71‑1 (MQ=255) tgatgatgaTCCCTATGAAAATGGCATCAGCATCGAAGATCAAGACt < 1:1564374/47‑1 (MQ=255) tgatgatgaTCCCCATGAAAATGGCATCAGCATCGAAGGTCAAGACTTTGCAGCAAAt > 1:1470777/1‑58 (MQ=255) tgatgatgaTCCCCATGAAAATGGCATCAGCATCGAAGATCAAGACTTTGCAGCAAATCTCTTCCGTGTTg < 1:1426457/71‑1 (MQ=255) gatgatgatCCCCATGAAAATGGCATCAGCATCGAAGATCAAGACTTTgcagca < 1:252173/54‑1 (MQ=255) catgatgatCCCCATGAAAATGGCATCAGCATCGAAGa > 1:660099/2‑38 (MQ=255) atgatgatCCCCATGAAAATGGCATCAGCATCGAAGATCAAGa > 1:609985/1‑43 (MQ=255) atgatCCCCATGAAAATGGCATCAGCATCGAAGATCAAGa > 1:23974/1‑40 (MQ=255) atgatCCCCATGAAAATGGCATCAGCATCGAAGATCAAGACtt < 1:4415558/43‑1 (MQ=255) tgatCCCCATGAAAATGGCATCAGCATCGAAGATCAAGACTTTGCAGCAAATCTCTTCCGTGTTGCTCGGa < 1:3145247/71‑1 (MQ=255) atCCCCATGAAAATGGCATCAGCATCGAAGATCAAGACTTTGCAGCaaa > 1:2289000/1‑49 (MQ=255) cccATGAAAATGGCATCAGCATCGAAGATCAAGACTTTGCAGCaa < 1:2284133/45‑1 (MQ=255) cATGAAAATGGCATCAGCATCGAAGATCAAGACTTTGCAGCAAATCTCTTCCGTGTTGCTCGGAAATGTCt > 1:2932687/1‑71 (MQ=255) aTGAAAATGGCATCAGCATCGAAGGTCAAGACTTTg < 1:4290561/36‑1 (MQ=255) aTGAAAATGGCATCAGCATCGAAGATCAAGACTTTgcagca < 1:1823155/41‑1 (MQ=255) tGAAAATGGCATCAGCATCGAAGGTCAAGACTTTGCAGCAAATCTCTTCCGTGtt > 1:1790554/1‑55 (MQ=255) tGAAAATGGCATCAGCATCGAAGATCAAGACTTTGCAGCAAATctct > 1:2498518/1‑47 (MQ=255) tGAAAATGGCATCAGCATCGAAGATCAAGACTTTGCAGCAAATCTCTTCCGTGTTg < 1:1755400/56‑1 (MQ=255) tGAAAATGGCATCAGCATCGAAGATCAAGACTTTGCAGCAAATCTCTTCCGTGTTGCTCGGAAATGTc < 1:4630225/68‑1 (MQ=255) tGAAAATGGCATCAGCATCGAAGATCAAGACTTTGCAGCAAATCTCTTCCGTGTTGCTCGGAAATGTCttt < 1:793642/71‑1 (MQ=255) gAAAATGGCATCAGCATCGAAGATCAAGACTTTGCAGCAAATCTCTTCCGTGTTGCTCGGAAATGTCttt > 1:1350517/1‑70 (MQ=255) gAAAATGGCATCAGCATCGAAGATCAAGACTTTGCAGCAAATCTCTTCCGTGTTGCTCGGAAATGTCttt > 1:1987635/1‑70 (MQ=255) aaaaTGGCATCAGCATCGAAGATCAAGACTTTGCAGCAAATCTCTTCCGTGTTGCTCGGAAATGTCtt > 1:1576829/1‑68 (MQ=255) aaaaTGGCATCAGCATCGAAGATCAAGACTTTGCAGCAAATCTCTTCCGTGTTGCTCGGAAATGTCTTTc > 1:1405621/1‑70 (MQ=255) aaaaTGGCATCAGCATCGAAGATCAAGACTTTGCAGCAAATCTCTTCCGTGTTGCTCGGAAATGTCTTTc > 1:1429853/1‑70 (MQ=255) aaaaTGGCATCAGCATCGAAGATCAAGACTTTGCAGCAAATCTCTTCCGTGTTGCTCGGAAATGTCTTTc > 1:385402/1‑70 (MQ=255) aaaTGGCATCAGCATCGAAGATCAAGACTTTGCAGCAAATCTCTTCCGTGTTGCTCGGAAATGTCTTTCaa < 1:983103/71‑1 (MQ=255) aTGGCATCAGCATCGAAGATCAAGACTTTGCAGCaa > 1:2403417/1‑36 (MQ=255) tGGCATCAGCATCGAAGATCAAGACTTTGCAGCAAATCTCTTCCGTGTTGCTCGGAAATGTCTTTCAACgg > 1:1871490/1‑71 (MQ=255) ggCATCAGCATCGAAGATCAAGACTTTGCAGCAAATCTCTTCCGTGTTGCTCGGa < 1:1734665/55‑1 (MQ=255) cATCAGCATCGAAGATCAAGACTTTGCAGCAAATCTCTTCCGTGTTGCTCGGAAATGTCTTTCAACggg > 1:1131772/1‑69 (MQ=255) cAGCATCGAAGATCAAGACTTTGCAGCAAATCTCTTCCGTGTTGCTCGGAAATGTCTTTCAAc < 1:2547788/63‑1 (MQ=255) gCATCGAAGATCAAGACTTTGCAGCAAATCTCTTCCGTGTTGCTCGGAAATGTCTTTCAACGGGAAGGCtt < 1:4488526/71‑1 (MQ=255) tCGAAGATCAAGACTTTGCAGCAAATCTCTTCCGTGTTGCTCGGATATGTCTTTCAACGGGAAGGCTtgat < 1:4591363/71‑1 (MQ=255) cGAAGATCAAGACTTTGCAGCAAATCTCTTCCgtt < 1:4266905/35‑2 (MQ=255) gAAGATCAAGACTTTGCAGCAAATCTCTTCCGTGTTGCTCGGAAATGTCTTTCAACGGGAAGGCTtgatga < 1:2247161/71‑1 (MQ=255) gAAGATCAAGACTTTGCAGCAAATCTCTTCCGTGTTGCTCGGAAATGTCTTTCAACGGGAAGGCTtgatg < 1:2157851/70‑1 (MQ=255) aGATCAAGACTTTGCAGCAAATCTCTTCCGTGTTGCTCGGAAATGTCTTTCAACGGGAAGGCTTGATGACg < 1:930679/71‑1 (MQ=255) aTCAAGACTTTGCAGCAAATCTCTTCCGTGTTGCTCGGa < 1:4573157/39‑1 (MQ=255) aTCAAGACTTTGCAGCAAATCTCTTCCGTGTTGCTCGGa < 1:2814383/39‑1 (MQ=255) aTCAAGACTTTGCAGCAAATCTCTTCCGTGTTGCTCGGa < 1:2549285/39‑1 (MQ=255) aTCAAGACTTTGCAGCAAATCTCTTCCGTGTTGCTCGGAAATGTCtt < 1:2010360/47‑1 (MQ=255) aTCAAGACTTTGCAGCAAATCTCTTCCGTGTTGCTAGGAAATGTCt < 1:1862718/46‑1 (MQ=255) | AAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGATCAAGACTTTGCAGCAAATCTCTTCCGTGTTGCTCGGAAATGTCTTTCAACGGGAAGGCTTGATGACG > W3110S.gb/2394005‑2394140 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |