| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,400,870 | T→G | 19.0% | D84A (GAC→GCC) | nuoI ← | NADH:ubiquinone oxidoreductase, chain I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,400,870 | 0 | T | G | 19.0% | 26.8 / 6.0 | 21 | D84A (GAC→GCC) | nuoI | NADH:ubiquinone oxidoreductase, chain I |
| Reads supporting (aligned to +/- strand): ref base T (13/4); new base G (2/2); total (15/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01 | |||||||||||
TTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTA > W3110S.gb/2400803‑2400939 | ttcGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCtt > 1:711213/1‑71 (MQ=255)ttcGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGGCtt > 1:28126/1‑71 (MQ=255) cGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCttt < 1:1947829/70‑1 (MQ=255) acaGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCtt < 1:2185155/71‑1 (MQ=255) aaaTGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTg > 1:4434908/1‑70 (MQ=255) ttGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGAc > 1:977134/1‑71 (MQ=255) gATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCgaga > 1:1266758/1‑59 (MQ=255) gATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGAc > 1:3933558/1‑69 (MQ=255) gATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCg > 1:3722818/1‑71 (MQ=255) aTGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATa > 1:4623492/1‑60 (MQ=255) aTGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATa > 1:2139692/1‑60 (MQ=255) cGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCggg > 1:2013031/1‑69 (MQ=255) gAAAAATTCCGGGTACCAGCGACCGGCTTTGGTTTCt < 1:3941345/37‑1 (MQ=255) aTTCCGGGTACCAGCGACCGGCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTa > 1:3999218/1‑69 (MQ=255) ttCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATa > 1:3657782/1‑49 (MQ=255) ttCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATa > 1:1113431/1‑49 (MQ=255) tCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGAc > 1:1789673/1‑56 (MQ=255) cGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACcgcg < 1:1029490/70‑1 (MQ=255) gggTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCa < 1:3809291/71‑1 (MQ=255) ggTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGAc > 1:4134529/1‑52 (MQ=255) cAGCGACCGGCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGtt < 1:1055749/71‑1 (MQ=255) tCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTa > 1:1972176/1‑70 (MQ=255) | TTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTA > W3110S.gb/2400803‑2400939 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |