| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,405,008 | C→T | 22.2% | E406E (GAG→GAA) | nuoF ← | NADH:ubiquinone oxidoreductase, chain F |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,405,008 | 0 | C | T | 22.2% | 70.4 / 15.5 | 36 | E406E (GAG→GAA) | nuoF | NADH:ubiquinone oxidoreductase, chain F |
| Reads supporting (aligned to +/- strand): ref base C (10/18); new base T (5/3); total (15/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
TTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCT > W3110S.gb/2404940‑2405072 | ttGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGTTc < 1:4003792/71‑1 (MQ=255) gCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCa > 1:853934/1‑71 (MQ=255) gCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCa > 1:2482847/1‑71 (MQ=255) cTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCa < 1:4500757/70‑1 (MQ=255) cTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCa < 1:228507/70‑1 (MQ=255) cTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCa < 1:164809/70‑1 (MQ=255) cTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCAc > 1:1898450/1‑71 (MQ=255) gAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTc < 1:1321708/66‑1 (MQ=255) cGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCAc > 1:1302374/1‑71 (MQ=255) tttGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAg < 1:1910070/68‑1 (MQ=255) tttGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAg < 1:1904684/68‑1 (MQ=255) tttGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTg > 1:1201278/1‑71 (MQ=255) ttGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCAc > 1:3295688/1‑59 (MQ=255) ttCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGTTCCACTGCAc < 1:2246123/60‑1 (MQ=255) cccGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGTTCCACTGCACCa > 1:3601893/1‑60 (MQ=255) cccGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGAGTggg < 1:296495/70‑1 (MQ=255) tCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCa > 1:2644365/1‑46 (MQ=255) ttcttcGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGTGCACAGAAAGttt < 1:1008204/71‑1 (MQ=255) ttcttcGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGttt < 1:394533/71‑1 (MQ=255) cttcGCGGAAATATTTGATGGCGCTCTGTAACGGTTCCACt > 1:1287880/1‑41 (MQ=255) cttcGCGGAAATATTTGATGGCGCTCTGTAACGGTTCCACt > 1:3781570/1‑41 (MQ=255) cttcGCGGAAATATTTGATGGCGCTCTGTAACGGTTCCACt > 1:2990731/1‑41 (MQ=255) cgcgGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTa < 1:3560205/68‑1 (MQ=255) gAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCAcc < 1:2341212/39‑1 (MQ=255) aaTATTTGATGGCGCTCTGTAACGGTTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCt < 1:2161054/71‑1 (MQ=255) atatTTGATGGCGCTCTGTAACGGTTCCACTGCACCAGGt > 1:4099175/1‑40 (MQ=255) atatTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTAccc > 1:2385450/1‑64 (MQ=255) tttGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTAc > 1:832158/1‑59 (MQ=255) tttGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAg < 1:2119242/70‑1 (MQ=255) tGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGc < 1:1310716/37‑1 (MQ=255) aTGGCTCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATc < 1:1461139/70‑1 (MQ=255) aTGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATc < 1:655954/70‑1 (MQ=255) cgcTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGacac < 1:504671/71‑1 (MQ=255) tGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGt < 1:1364078/41‑1 (MQ=255) gTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGa < 1:1622177/57‑1 (MQ=255) aaCGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCt > 1:3720850/1‑70 (MQ=255) | TTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCT > W3110S.gb/2404940‑2405072 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |