Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 2,413,837 T→C 32.3% C277C (TGT→TGC yfbQ → predicted aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,413,8370TC32.3% 70.1 / 53.2 62C277C (TGT→TGCyfbQpredicted aminotransferase
Reads supporting (aligned to +/- strand):  ref base T (19/23);  new base C (3/17);  total (22/40)
Fisher's exact test for biased strand distribution p-value = 2.46e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-01

TGAACGGGCCGAAAAAACACGCCAAAGGCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAGGTGGTTATCAGAGCATCAGTGAATTTAT  >  W3110S.gb/2413767‑2413905
                                                                      |                                                                    
tGAACGGGCCGAAAAAACACGCCAAAGGCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCtgtgt                                                                      >  1:2058800/1‑71 (MQ=255)
  aaCGGGCCGAAAAAACACGCCAAAGGCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGc                                                                    >  1:3400444/1‑71 (MQ=255)
    cGGGCCGAAAAAACACGCCAAAGGCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCt                                                                   >  1:1202076/1‑70 (MQ=255)
     gggCCGAAAAAACACGCCAAAGGCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTaa                                                                 >  1:1686245/1‑71 (MQ=255)
     gggCCGAAAAAACACGCCAAAGGCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTaa                                                                 >  1:1646261/1‑71 (MQ=255)
         cGAAAAAACACGCCAAAGGCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGtt                                                             >  1:3241516/1‑71 (MQ=255)
           aaaaaaCACGCCAAAGGCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTcc                                                           >  1:2118607/1‑71 (MQ=255)
           aaaaaaCACGCCAAAGGCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTcc                                                           <  1:1676260/71‑1 (MQ=255)
                acacGCCAAAGGCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTgcgc                                                      <  1:3111599/71‑1 (MQ=255)
                  acGCCAAAGGCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGGCTGTGCGCTa                                                                  <  1:252662/57‑1 (MQ=39)
                  acGCCAAAGGCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCaa                                                    <  1:3723667/71‑1 (MQ=255)
                  acGCCAAAGGCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGCGCTa                                                                  <  1:1537331/57‑1 (MQ=255)
                  acGCCAAAGGCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGCGCTAACGTTCCTGCGCaa                                                    <  1:1654897/71‑1 (MQ=255)
                  acGCCAAAGGCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGCGCTAACGTTCCTGCGCaa                                                    <  1:3729916/71‑1 (MQ=255)
                      cAAAGGCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGCGCTAACGTTCCTGCGCAACACg                                                <  1:197126/71‑1 (MQ=255)
                        aaGGCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGcc                                              <  1:1202979/71‑1 (MQ=255)
                         aGGCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCaa                                                    >  1:3274796/1‑64 (MQ=255)
                           gCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGCGCTAACGTTCCTGCGCAACACGCCAtt                                           <  1:3356881/71‑1 (MQ=255)
                           gCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGCGCTAACGTTCCTGCGCAACACGCCAtt                                           <  1:1547878/71‑1 (MQ=255)
                            cTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTgcgc                                                      <  1:1042872/59‑1 (MQ=255)
                            cTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTc                                          <  1:3532752/71‑1 (MQ=255)
                               cATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAg                                        <  1:2599707/70‑1 (MQ=255)
                                aTCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGCGCt                                                                   <  1:2710861/42‑1 (MQ=255)
                                   gAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCa                                             >  1:2716336/1‑61 (MQ=255)
                                   gAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCa                                             >  1:4385379/1‑61 (MQ=255)
                                   gAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACcgc                                   <  1:1340536/71‑1 (MQ=255)
                                   gAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACcgc                                   <  1:2079938/71‑1 (MQ=255)
                                   gAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACcgc                                   <  1:1214131/71‑1 (MQ=255)
                                   gAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGCGCTAACGTTCCTGCGCAACACGCCATTCAGACagc                                   >  1:3206291/1‑71 (MQ=255)
                                      ggTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCt                                <  1:3095314/71‑1 (MQ=255)
                                      ggTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCt                                <  1:2678261/71‑1 (MQ=255)
                                      ggTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCt                                <  1:980036/71‑1 (MQ=255)
                                         cTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACg                                                               >  1:1338312/1‑37 (MQ=255)
                                         cTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCa                                         >  1:2560911/1‑59 (MQ=255)
                                         cTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCa                                         >  1:4487770/1‑59 (MQ=255)
                                          tGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAAc                                                                <  1:1836622/35‑1 (MQ=255)
                                          tGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCa                                             <  1:1649821/54‑1 (MQ=255)
                                          tGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTa                               <  1:3701504/68‑1 (MQ=255)
                                           ggAAATGCTGTCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCa                                             <  1:762218/53‑1 (MQ=255)
                                           ggAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCaa                                                    <  1:4155144/46‑1 (MQ=255)
                                            gAAATGCTGGCTTCAATGCGCCTGTGCGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAggtgg                          >  1:2609350/1‑71 (MQ=255)
                                            gAAATGCTGGCTTCAATGCGCCTGTGCGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAggt                            <  1:3193562/69‑1 (MQ=255)
                                            gAAATGCTGGCTTCAATGCGCCTGTGCGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAgg                             <  1:3581902/68‑1 (MQ=255)
                                             aaaTGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGc                                               >  1:2012941/1‑49 (MQ=255)
                                              aaTGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAggtggt                         >  1:3697805/1‑70 (MQ=255)
                                               aTGCTGGCTTCAATGCGCCTGTGCGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAgg                             <  1:1623665/65‑1 (MQ=255)
                                               aTGCTGGCTTCAATGCGCCTGTGCGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAgg                             <  1:1470265/65‑1 (MQ=255)
                                               aTGCTGGCTTCAATGCGCCTGTGCGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAgg                             <  1:3609467/65‑1 (MQ=255)
                                                tGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAGGTGGTTAt                      <  1:1435078/71‑1 (MQ=255)
                                                tGCTGGCTTCAATGCGCCTGTGCGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAgg                             <  1:1667088/64‑1 (MQ=255)
                                                tGCTGGCTTCAATGCGCCTGTGCGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAGGTGGTTAt                      <  1:1945848/71‑1 (MQ=255)
                                                 gCTGGCTTCAATGCGCCTGTTCGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAGGTGGTTAt                      >  1:553523/1‑70 (MQ=255)
                                                    ggCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAGGTGGTTATCa                    >  1:4534901/1‑69 (MQ=255)
                                                    ggCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAGGTGGTTATCa                    >  1:289533/1‑69 (MQ=255)
                                                     gCTTCAATGCGCCTGTGCGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAGGTGGTTATCag                   <  1:971060/69‑1 (MQ=255)
                                                      cTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAGGTGGTTATCAGAGc                <  1:2978616/71‑1 (MQ=255)
                                                         cAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGAc                                      >  1:2322476/1‑46 (MQ=255)
                                                          aaTGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTc                                          <  1:4609003/41‑1 (MQ=255)
                                                           aTGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAGGTGGTTATCAGAGCATCAg           <  1:1915642/71‑1 (MQ=255)
                                                              cgcCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAGGTGGTTATCAGAGCATCAg           >  1:2348760/1‑68 (MQ=255)
                                                                ccTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAGGTGGTTATc                     >  1:475666/1‑56 (MQ=255)
                                                                 cTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAGGTGGTTATCAGAGCATCAGTGAAtt     <  1:416142/71‑1 (MQ=255)
                                                                 cTGTGCGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAGGTGGTTATCAGAGCATCAGTGAAtt     <  1:3940351/71‑1 (MQ=255)
                                                                  tgtgtgCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAgg                             <  1:3206183/46‑1 (MQ=255)
                                                                  tgtgtgCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAGGTGGTTATCAGAGCATCAGTGAAtt     >  1:3496917/1‑70 (MQ=255)
                                                                    tgtgCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAggtg                           <  1:1603503/46‑1 (MQ=255)
                                                                    tgtgCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAg                              >  1:3361045/1‑43 (MQ=255)
                                                                    tgtgCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAGGTGGTTAt                      <  1:4525588/51‑1 (MQ=255)
                                                                    tgtgCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAGGTGGTTATCAGAGCATCAGTGAAtt     <  1:2601429/68‑1 (MQ=255)
                                                                    tgtgCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAGGTGGTTATCAGAGCATCAGTGAAtt     <  1:3822184/68‑1 (MQ=255)
                                                                    tgtgCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAGGTGGTTATCAGAGCATCAGTGAATttat  >  1:1356163/1‑71 (MQ=255)
                                                                      |                                                                    
TGAACGGGCCGAAAAAACACGCCAAAGGCTACATCGAAGGTCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTAGGTGGTTATCAGAGCATCAGTGAATTTAT  >  W3110S.gb/2413767‑2413905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: